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Method for preparing novel magneto-induction degradable nervous tissue engineering material

A technology of nerve tissue and engineering materials, applied in medical science, prostheses, etc., can solve problems such as slowing down the growth rate of nerves, poor functional recovery of innervation areas, poor repairing effect of nerve scaffolds, etc., and achieve the effect of improving the repairing effect

Inactive Publication Date: 2014-12-17
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a preparation method of a new type of magnetically inductive degradable nerve tissue engineering material, which solves the problem that the growth direction of the regenerated nerve is uncertain, which makes the repair effect of the existing nerve support poor, slows down the growth rate of the nerve, and leads to poor functional recovery of the innervation area. question

Method used

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  • Method for preparing novel magneto-induction degradable nervous tissue engineering material
  • Method for preparing novel magneto-induction degradable nervous tissue engineering material
  • Method for preparing novel magneto-induction degradable nervous tissue engineering material

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Effect test

Embodiment 1

[0029](1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and mechanically stir for 3 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 9, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 °C, vacuum-dried, ground and sieved to obtain MNPs.

[0030] (2) Weigh chitosan and sodium β-glycerophosphate respectively, the masses of chitosan and sodium β-glycerophosphate are 50 mg and 5 mg respectively, and weigh 5 mg of MNPs prepared in (1).

[0031...

Embodiment 2

[0036] (1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and stir mechanically for 3.5 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 9.5, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 °C, vacuum-dried, ground and sieved to obtain MNPs.

[0037] (2) Weigh chitosan and sodium β-glycerophosphate respectively, the masses of chitosan and sodium β-glycerophosphate are 50 mg and 5 mg respectively, and weigh 10 mg of MNPs prepared in (1).

...

Embodiment 3

[0043] (1) Prepare 0.1mol / L Fe respectively 3+ and Fe 2+ solution, under nitrogen, according to Fe 3+ :Fe 2+ Mix at a molar ratio of 2:1, and mechanically stir for 4 hours to make it fully mixed. Under the condition of continuous nitrogen flow, 1 mol / L NaOH solution was added dropwise to the mixed solution until the pH of the mixed solution was 10, and a black precipitate was obtained. Then the water bath was heated for 2h, and then after standing for 5h, the supernatant was removed, the generated particles were filtered under reduced pressure, washed repeatedly with distilled water and absolute ethanol, and the impurity ions on the surface of the particles were washed away, and the obtained particles were obtained at 50 °C, vacuum-dried, ground and sieved to obtain MNPs.

[0044] (2) Weigh chitosan and sodium β-glycerophosphate respectively, the masses of chitosan and sodium β-glycerophosphate are 50 mg and 5 mg respectively, and weigh 20 mg of MNPs prepared in (1).

[0...

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Abstract

The invention discloses a method for preparing a novel magneto-induction degradable nervous tissue engineering material. The technical scheme of the method is carried out in the following steps: 1, preparing magnetic nanoparticles MNPs; 2, preparing a magnetic gel-shaped turbid liquid; 3, preparing a nervous tissue engineering material; and 4, carrying out crosslinking disinfection treatment. According to the magneto-induction degradable nervous tissue engineering material prepared by the method, a generated nerve grows in an oriented manner by the magnetic field inside the tissue material to accelerate repairing and the forming of myelin sheath, and the effect of repairing nerve defect can be further improved.

Description

technical field [0001] The invention belongs to the technical field of long-segment peripheral nerve injury repair, and relates to a preparation method of a novel magnetic induction degradable nerve tissue engineering material. Background technique [0002] Peripheral nerve defect is one of the most common clinical injuries. This type of nerve injury will cause complete loss of both sensory and motor functions in the distal limbs innervated by the affected nerves, resulting in severe disability, which will have a huge impact on the patient's work and quality of life. [0003] At present, clinically, for short-distance peripheral nerve defects, on the basis of the principle of tension-free suture, the method of direct suture of nerve stumps is often used for repair; for long-distance peripheral nerve defects, autologous nerve transplantation is often used. method to repair. However, studies have shown that: autologous nerve transplantation can only achieve partial recovery ...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/20A61L27/50
Inventor 刘鐘阳黄景辉罗卓荆朱澍刘靓
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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