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Petroleum endogenous microbe activating system, and screening method and application thereof

An endogenous microorganism and screening method technology, which is applied to the oil endogenous microorganism activation system and its screening field, can solve the problems of inappropriate activation material ratio, environmental pollution of activation material, and difficulty in obtaining reagents, etc. Does not pollute the environment and reduces viscosity

Inactive Publication Date: 2014-12-17
北京大学包头创新研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The technical problems solved by the present invention are: in the prior art, there are problems such as high cost, long cultivation time, inappropriate proportioning of activating substances, environmental pollution caused by addition of activating substances, difficulty in obtaining some reagents or certain dangers in storage, etc.

Method used

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  • Petroleum endogenous microbe activating system, and screening method and application thereof
  • Petroleum endogenous microbe activating system, and screening method and application thereof
  • Petroleum endogenous microbe activating system, and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The present invention screens the activator components through a single factor experiment, and determines the appropriate concentration range of the activator. The specific experimental process is as follows:

[0072] For the experiment of determining the type of activator for petroleum endogenous microorganisms, corn steep liquor dry powder, molasses or sucrose were selected as carbon sources, and Na 2 HPO 4 、K 2 HPO 4 or (NH 4 ) 2 HPO 4 as phosphorus source, NaNO 3 、KNO 3 or NH 4 NO 3 As a nitrogen source (and as an inhibitor of sulfate-reducing bacteria), single-factor experiments were carried out to determine the optimal concentration range of carbon, phosphorus and nitrogen sources.

[0073] Determination method: Add 100mL inorganic salt solution and the influencing factors to be investigated into a 150ml Erlenmeyer flask, make three parallel samples, sterilize at 121°C for 30 minutes, add 4g of Changqing oilfield crude oil, and culture at 30°C, shaker 120...

Embodiment 2

[0076] The present invention uses Plackett-Burman experiment to measure the impact of the components of different activation systems on the bacterial concentration, determine the most significant activator that affects the bacterial concentration, and determine the optimum concentration that produces the impact. The specific experimental process is as follows:

[0077] Plackett-Burman experiment is based on the concentration of endogenous bacterial flora OD 600 In order to detect the indicators, crude oil from Changqing Oilfield was used to investigate the growth and activation of endogenous bacterial flora by different activators, and the design and analysis software of Design experts V8.0 was used for data processing and analysis, and the significant influence of the factors under investigation on the indicators was obtained. sexual situation.

[0078] Experiments on molasses, K 2 HPO 4 , NH 4 Cl, NaNO 3 , MgCl 2 , CaCl 2 Six factors were used as activator components f...

Embodiment 3

[0082] The present invention determines and verifies the optimization result through CCD experiment, and concrete experiment process is as follows:

[0083] Select the fourth experimental point of the above climbing experiment (molasses: 3.0g / L; K 2 HPO 4 : 3.96g / L; NH 4 Cl: 2.82g / L) as the center point, design a group of experiments to investigate the optimal concentration of the activator and the influence of various factors, compare the fitted value with the measured value, and determine the optimal result as, the best activation system The concentration of each component is: molasses content is 3g / L, K 2 HPO 4 4g / L and NH 4 Cl is 2.5 g / L.

[0084] Add 100mL activation system to the 150mL Erlenmeyer flask, wherein the molasses content is 3g / L, K 2 HPO 4 4g / L; NH 4 The Cl was 2.5 g / L, and three parallel samples were made. After sterilizing at 121°C for 30 minutes, 4 g of crude oil from Changqing Oilfield was added. 30°C, 120rpm / min, shaking culture. Sampling after ...

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Abstract

The invention relates to the field of petroleum exploitation, and concretely relates to a petroleum endogenous microbe activating system, and a screening method and application thereof. The provided petroleum endogenous microbe activating system comprises an inorganic salt solution and an activator, wherein the activator comprises carbon source, phosphorus source and nitrogen source activators, the carbon source activator comprises molasses, the phosphorus source activator comprises K2HPO4, and the nitrogen source activator is one or more selected from NaNO3, NH4Cl or NH4NO3. The provided screening method for the petroleum endogenous microbe activating system comprises the following steps: (1) using the inorganic salt solution to stimulate stratum water, using a single-factor experiment to screen activator compositions and concentration scope; and (2) using Plackett-Burman, climbing experiments and CCD experiments to screen out all important compositions in the activator and the optimum concentration of all compositions. The application of the petroleum endogenous microbe activating system to fields of petroleum degradation and petroleum exploitation also is provided. The activating system is cheap and efficient, and the screening method is simple and rapid, and is capable of effectively improving the petroleum recovery ratio.

Description

technical field [0001] The invention relates to the field of petroleum exploitation, especially the field of activation of endogenous microbes in oil reservoirs, in particular to a petroleum endogenous microbe activation system and a screening method and application thereof. Background technique [0002] The types and quantities of endogenous microorganisms in oil reservoirs may not be many, mainly due to the limitation of nutrients in the formation. If appropriate nutrients are provided, these endogenous microorganisms can be activated and multiply in large numbers in oil reservoirs. According to the research of Jianghan Petroleum Institute, microorganisms generally require phosphorus-containing compounds (such as various organic and inorganic phosphates), nitrogen-containing compounds (such as ammonium chloride and other ammonium-containing compounds, potassium nitrate and other nitrates, and amino acids and peptides) organic nitrogen), carbon compounds (such as fats, prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K8/582E21B43/22
CPCC09K8/582
Inventor 来国莉蔚晓燕刘晓霞池昌桥聂勇赵雅坤李卫乐吴晓磊
Owner 北京大学包头创新研究院
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