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Method for quickly reproducing hygrophila salicifolia callus tissues and suspension cells

A technology of callus and suspension cells, which is applied in the field of plants, can solve the problems of little research and no research on water raincoats, and achieve the effects of high proliferation rate, low energy consumption and simple operation

Inactive Publication Date: 2015-01-07
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of harvesting is field collection, and the main method of reproduction is natural reproduction. At present, there is still little research on it, and no one has studied the application of tissue culture technology to the reproduction of water coir raincoats.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the leaves of the water coir raincoat, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 35 minutes, treat them with sodium hypochlorite on the ultra-clean workbench for 14 minutes, rinse them with sterile water 5-7 times, insert the sterilized water coir raincoat leaves The medium formula is improved MS+KT0.5mg / L+2, 4-D2.0mg / L+ sodium thiosulfate 100mg / L medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L , PH5.8, light intensity 1000lx, temperature 23°C, humidity 70%-80%, the induced callus access medium formula is MS+KT0.2mg / L+2,4-D0.2mg / L+folic acid Culture in the proliferation medium of 0.5mg / L + 3% sucrose + 0.6% agar, pH 5.8, light intensity 1500lx, temperature 23°C, humidity 70%-80%, the callus with good dispersion after proliferation is put into liquid culture Base MS+2,4-D0.2mg / L+KT0.4mg / L+5mg / L phenylalanine for shaking culture, add 30g / L sucrose, inoculate 30ml li...

Embodiment 2

[0011] Take the leaves of the water coir raincoat, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 35 minutes, treat them with sodium hypochlorite on the ultra-clean workbench for 14 minutes, rinse them with sterile water 5-7 times, insert the sterilized water coir raincoat leaves The medium formula is improved MS+KT0.5mg / L+2, 4-D2.0mg / L+ sodium thiosulfate 100mg / L medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L , PH5.8, light intensity 1000lx, temperature 23°C, humidity 70%-80%, the induced callus access medium formula is MS+KT0.2mg / L+2,4-D0.2mg / L+folic acid Culture in the proliferation medium of 0.5mg / L + 3% sucrose + 0.6% agar, pH 5.8, light intensity 1500lx, temperature 23°C, humidity 70%-80%, the callus with good dispersion after proliferation is put into liquid culture Base MS+2,4-D0.3mg / L+KT0.6mg / L+6mg / L phenylalanine for shaking culture, add 30g / L sucrose, inoculate 30ml li...

Embodiment 3

[0013] Take the leaves of the water coir raincoat, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 35 minutes, treat them with sodium hypochlorite on the ultra-clean workbench for 14 minutes, rinse them with sterile water 5-7 times, insert the sterilized water coir raincoat leaves The medium formula is improved MS+KT0.5mg / L+2,4-D2.0mg / L+thiosulfuric acid 100mg / L medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L, PH5.8, light intensity 1000lx, temperature 23°C, humidity 70%-80%, the induced callus is inserted into the medium formula as MS+KT0.2mg / L+2, 4-D0.2mg / L+folate 0.5 Cultivate in the proliferation medium of mg / L + 3% sucrose + 0.6% agar, pH 5.8, light intensity 1500lx, temperature 23°C, humidity 70%-80%, callus with good dispersion after proliferation are placed in liquid medium Shaking culture in MS+2,4-D0.3mg / L+KT0.4mg / L+6mg / L phenylalanine, add 30g / L sucrose, inoculate 30ml l...

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PUM

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Abstract

The invention provides a method for quickly reproducing hygrophila salicifolia callus tissues and suspension cells. The method comprises the following steps: preparing sterile leaves; inducing callus tissues; multiplying callus tissues; culturing suspension cells and the like. The method can be used for culturing hygrophila salicifolia cells in a large scale and producing secondary metabolite and is capable of overcoming the defect that a chemical method is not uneconomical in synthesis and is influenced by natural and artificial conditions. The method disclosed by the invention has the advantages of simplicity in operation, short growing cycle and high multiplication rate.

Description

technical field [0001] The invention relates to the cultivation of water coir raincoat suspension cells under tissue culture conditions, and belongs to the technical field of plants. Background technique [0002] water cape, Hygrop Hila salicifolia (Vahl), herb, also known as piercing snake, fish bone grass, sweet and slightly bitter, can be used as medicine. The genus Acanthaceae is used as medicine with the whole plant. Can be harvested throughout the year, used fresh, or washed and dried. Functions and indications: heat-clearing and toxic substances removing, removing blood stasis and relieving pain. For pharyngitis, mastitis, hematemesis, epistaxis, whooping cough; for external use to treat fractures, bruises, and venomous snake bites. Produced in Guangdong, Guangxi, Hainan, Taiwan, Hong Kong, Fujian, Jiangxi, Zhejiang, Anhui, Hunan, Hubei, Sichuan, Yunnan and other provinces and regions. Growing in moist places such as streamsides or depressions. It is distributed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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