Camellia nitidissima saponin A, and preparation method and antitumor application thereof

A technology of tea saponin and golden camellia, which is applied in the field of camellia saponin A compound, can solve the problems of rough process, complex components, and inability to determine the composition and structure of components

Inactive Publication Date: 2015-01-07
GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR ANALYSIS & TEST RES +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, due to the relatively rough process of the research in this document, although four saponin components such as WS, IS1, IS2, and IS3 have b

Method used

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  • Camellia nitidissima saponin A, and preparation method and antitumor application thereof
  • Camellia nitidissima saponin A, and preparation method and antitumor application thereof
  • Camellia nitidissima saponin A, and preparation method and antitumor application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0052] One, the preparation of camellia saponin A

[0053] 1. Instruments and reagents

[0054] Instrument: Intelligent Ultrasonic Extractor (Shanghai Zhixin Instrument Co., Ltd.).

[0055] Rotary evaporator (Shanghai Yarong Biochemical Instrument Factory)

[0056] Waters2695 High Performance Liquid Chromatography (Waters, USA)

[0057] Nicolet 4700 Fourier transform infrared spectrometer (KBr tablet, Nicolet company)

[0058] UV spectrophotometer (Thermo company)

[0059] AV600 nuclear magnetic resonance spectrometer (Bruker, Switzerland)

[0060] High-resolution time-of-flight mass spectrometer (Bruker, Switzerland)

[0061] Reagent: XAD-16 macroporous adsorption resin (Tianjin Haiguang Chemical Co., Ltd.)

[0062] D101 Macroporous Adsorption Resin (Nankai University Chemical Plant)

[0063] AB-8 macroporous adsorption resin (Nankai University Chemical Plant)

[0064] HP-20 macroporous adsorption resin (Mitsubishi, Japan)

[0065] Silica gel for chromatographic colu...

Embodiment 1

[0072] Put the golden camellia raw materials that have been prepared from the Shiwandashan Lanshan area of ​​Fangchenggang City, Guangxi into the ultrasonic extractor, and put the methanol-water solution with a volume ratio of 50:50 according to the solid-liquid ratio of 1:10, and then put it under the condition of 40 ° C. Under the condition of leaching at 15KHz for 1h, the Camellia japonica extract was obtained.

[0073] Concentrate Camellia japonica extract, add water to dissolve, and absorb macroporous resin XAD-16 to obtain macroporous resin adsorbate.

[0074] The macroporous resin adsorbate was eluted with water for 5 column volumes, and then eluted with 70% ethanol, and the 70% ethanol part was collected and evaporated to dryness to obtain the total saponins of Camellia japonica; Separation by column chromatography, eluting with chloroform-methanol at a volume ratio of 15:1, and then taking the eluent for thin-layer chromatography detection, combining 123 to 162 fracti...

Embodiment 2

[0077] Put the prepared raw materials of Fangcheng Dawangjiang golden camellia tea into the ultrasonic extractor, put into the ethanol-water solution with a volume ratio of 55:45 according to the ratio of solid to liquid ratio of 1:12, and then soak at 50°C at 20KHz Extract for 2 hours to obtain Camellia Camellia extract.

[0078] Concentrate Camellia japonica extract, add water to dissolve, and absorb macroporous resin D101 to obtain macroporous resin adsorbate.

[0079] The macroporous resin adsorbate was eluted with water for 5 column volumes, and then eluted with 70% ethanol, and the 70% ethanol part was collected and evaporated to dryness to obtain the total saponins of Camellia japonica; Separation by column chromatography, eluting with chloroform-methanol with a volume ratio of 17:2, and then taking the eluent for thin-layer chromatography detection, combining 123-162 fractions and concentrating to obtain a concentrated solution, and concentrating the concentrated solut...

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Abstract

The invention relates to a new Camellia nitidissima saponin A compound separated from Camellia nitidissima and a preparation method thereof, and application of the compound in tumor resistance. The dammarane saponin new compound is separated from Camellia nitidissima. The new compound is named (3beta,6alpha,12beta)-3,6,12-trihydroxydammaryl-24-alkenyl-20-methyl-2-O-beta-D-glucopyranosyl-(2->1)-O-beta-D-glucopyranosyl-(2->1)-O-alpha-L-pyranylrhamnosido[(3beta,6alpha,12beta)-3,6,12-trihydroxydammar-24-en-20-yl-2-O-beta-D-glucopyranosyl-(2->1)-O-beta-D-glucopyranosyl-(2->1)-O-alpha-L-Rhamnopyranoside]. A CCk-8 process is utilized to carry out antitumor activity detection on the new compound; and the experimental result indicates that the compound has the activities for inhibiting human lung cancer BeL-7402 cells, SMMC-7721 and the like from growth.

Description

technical field [0001] The invention relates to a new camellia saponin A compound isolated from Camellia chrysanth (Hu) Tuyama, a preparation method thereof, and the antitumor application of the compound. Background technique [0002] Camellia chrysanth (Hu) Tuyama is a plant of the family Theaceae, the genus Camellia, the Camellia group, and the Camellia family. For evergreen shrubs or small trees. It is mainly produced in two discontinuous areas south of the Tropic of Cancer in Guangxi, my country. On July 29, 1933, Zuo Jinglie, a Chinese botanist, first discovered camellia camellia in Daludo Axiai, Fangcheng County, Guangxi. Among the 23 kinds of golden camellia, 21 grow in Fangchenggang, and golden camellia is listed as a national first-class protected rare plant. Camellia not only has high ornamental value, but also has high medicinal value. Containing a variety of health-care ingredients, it has been used by folks for clearing away heat and detoxification, diuresis a...

Claims

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Application Information

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IPC IPC(8): C07J17/00A61K31/7032A61P35/00
CPCC07J17/005
Inventor 黄艳莫建光韦英亮葛利潘艳坤苏琳
Owner GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR ANALYSIS & TEST RES
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