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A kind of cistanche polypeptide extract, preparation method and use thereof

The technology of Cistanche deserticola and its extract is applied in the field of medicine, and can solve the problems of unclear mechanism of action, difficulty in periodic chemotherapy, disorder of serum electrolytes, etc., and achieve the effects of avoiding environmental pollution problems, inhibiting the growth of cancer cells, and having high reproducibility.

Active Publication Date: 2017-08-04
内蒙古中兴华冠生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the advent and popularization of chemotherapy drugs such as 5-fluorouracil, taxanes, hydroxycamptothecin, irinotecan, Xeloda, etc., the incidence of chemotherapy-related diarrhea has increased year by year. Treatment can easily lead to dehydration, malnutrition, serum electrolyte disorder, acid-base balance disorder, etc., which not only bring great disadvantages to the recovery of the body, but also bring certain difficulties to periodic chemotherapy. Effective treatment has become an important part of the overall treatment of cancer patients
[0009] At present, there is no Chinese patent medicine that can relieve diarrhea and synergize anti-cancer chemotherapy. The mechanism of action of the Chinese medicine composition disclosed in Chinese patent CN103585546A in the treatment of diarrhea caused by cancer chemotherapy is not clear, and the drug effect is not obvious. Therefore, the Chinese medicine composition in this With the assistance of the medical team, it is particularly important to develop a pharmaceutical composition that is highly effective, low in toxicity, low in price, and easy to promote, which can be used to alleviate cancer chemotherapy-related diarrhea and play a synergistic role in chemotherapy.

Method used

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  • A kind of cistanche polypeptide extract, preparation method and use thereof
  • A kind of cistanche polypeptide extract, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, a kind of method of preparing Cistanche polypeptide extract from Cistanche powder:

[0035] (1) Add NaOH solution to Cistanche powder until the pH value is 10, extract at 45°C for 60 minutes, and then centrifuge to obtain Cistanche protein;

[0036] (2) Use the cistanche protein obtained in step (1) to configure a cistanche protein reaction system with a substrate concentration of 5%, adjust the pH value of the cistanche protein reaction system to 6.0, control the temperature at 70°C, and add an enzymatic protease with an enzyme-to-substrate ratio of 4.0% , enzymolysis with constant temperature stirring, the enzymolysis time is 4.0h, and the enzymolysis solution is obtained, and the enzymolysis protease is papain;

[0037] (3) Boil the enzymolysis solution obtained in step (2) for 17 minutes to inactivate the enzyme. After cooling, use 2 mol / L HCL solution to adjust the pH value to neutral, then centrifuge to remove the precipitate, take the supernatant, a...

Embodiment 2

[0040] Embodiment 2, a kind of method of preparing Cistanche polypeptide extract from Cistanche powder:

[0041] (1) Add NaOH solution to Cistanche powder until the pH value is 8, extract at 55°C for 40 minutes, and then centrifuge to obtain Cistanche protein;

[0042] (2) Use the cistanche protein obtained in step (1) to configure a cistanche protein reaction system with a substrate concentration of 9%, adjust the pH value of the cistanche protein reaction system to 7.0, control the temperature at 60°C, and add an enzymatic protease with an enzyme-to-substrate ratio of 3.0% , stirring at constant temperature for enzymolysis, the enzymolysis time is 3.0h, and the enzymolysis solution is obtained, and the enzymolysis protease is papain;

[0043] (3) Boil the enzymolysis solution obtained in step (2) for 15 minutes to inactivate the enzyme. After cooling, use 2 mol / L HCL solution to adjust the pH value to neutral, then centrifuge to remove the precipitate, take the supernatant, ...

Embodiment 3

[0046] Embodiment 3, a kind of method for preparing Cistanche polypeptide extract from Cistanche powder:

[0047] (1) Add NaOH solution to Cistanche powder until the pH value is 9, extract at 50°C for 50 minutes, and then centrifuge to obtain Cistanche protein;

[0048] (2) Use the cistanche protein obtained in step (1) to prepare a cistanche protein reaction system with a substrate concentration of 7%, adjust the pH value of the cistanche protein reaction system to 6.5, control the temperature at 65°C, and add an enzymatic protease with an enzyme-to-substrate ratio of 3.5% , enzymolysis with constant temperature stirring, the enzymolysis time is 3.5h, and the enzymolysis solution is obtained, and the enzymolysis protease is papain;

[0049] (3) Boil the enzymolysis solution obtained in step (2) for 15 minutes to inactivate the enzyme. After cooling, use 2 mol / L HCL solution to adjust the pH value to neutral, then centrifuge to remove the precipitate, take the supernatant, and...

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Abstract

The invention provides a method for extracting desertliving cistanche polypeptide extracts from desertliving cistanche, a desertliving cistanche polypeptide extract obtained according to the method, and use of the desertliving cistanche polypeptide extract. The method comprises the following steps: using desertliving cistanche residues, baking and crushing the desertliving cistanche residues, treating the baked and crushed desertliving cistanche residues by an alkali-solution and acid-isolation method, performing enzymolysis, ultrafiltration and purification, finally obtaining desertliving cistanche polypeptide of which the molecular weight is 500-10000 Dalton, and researching the efficacies of the desertliving cistanche polypeptide for treating cancers and diarrhoea due to cancer chemotherapy. According to the invention, the utilization of desertliving cistanche which belongs to rare medicinal materials in imminent danger, so that the desertliving cistanche has a higher economic value; moreover, the invention provides a medicine which has the advantages of high efficiency, low toxicity and low cost, and the medicine is easy to generalize, can be used for alleviating relevance diarrhoea of cancer chemotherapy and has a function of chemotherapeutic sensitivity.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a method for extracting a Cistanche polypeptide extract from Cistanche deserticola, and the Cistanche deserticola polypeptide extract obtained by the method and the application of the Cistanche deserticola polypeptide extract. Background technique [0002] Cistanche deserticola (Cistanche deserticola Y.C.Ma) is the dry succulent stem with scaly leaves of Cistanche deserticola (Orobanchaeceae) plant Cistanche. The reputation of "Desert Ginseng" was first recorded in "Shen Nong's Materia Medica", listed as top grade, and is one of the commonly used traditional Chinese medicines. There are about 18 species of this genus in the world, and there are about 4 species and 1 variant in my country, which are Cistanche, Salina, Cistanche, White-flowered Cistanche and Cistanche. Except Cistanche, the other 4 species are mainly produced in Provinces in Northwest my country. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P35/00A61P1/12
Inventor 孙喜翠
Owner 内蒙古中兴华冠生物工程股份有限公司