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A kind of composite immune enhancer for Macrobrachium rosenbergii and its application

An immune enhancer, the technology of Macrobrachium rosenbergii, applied in the application, animal feed, additional food elements, etc., can solve the problems of the decline of shrimp's own immunity, the threat of drug residues, and the large toxic and side effects, so as to enhance non-specific immune function, Stable source of raw materials and the effect of improving growth ability

Active Publication Date: 2019-03-12
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of drug resistance, large toxic and side effects, decreased immunity of shrimps, and threats to human health caused by drug residues in the large-scale use of antibiotics in the current Macrobrachium rosenbergii culture process, and provides a compound for Macrobrachium rosenbergii The immune enhancer has stable and wide sources of raw materials, low cost, no toxic and side effects, and is degradable under natural conditions without remaining in the shrimp body. It can enhance the non-specific immune function of Macrobrachium rosenbergii, and can also improve the Growth ability

Method used

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  • A kind of composite immune enhancer for Macrobrachium rosenbergii and its application
  • A kind of composite immune enhancer for Macrobrachium rosenbergii and its application
  • A kind of composite immune enhancer for Macrobrachium rosenbergii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Macrobrachium rosenbergii is prepared with a compound immune enhancer: Tremella polysaccharide 25g, baicalin 10g, tea tree mushroom polysaccharide 35g, levamisole 10g, vitamin C 20g; after mixing evenly, a compound immune enhancer is obtained, and 0.05% (accounting for Roche marsh Shrimp compound feed (by weight) is added to Macrobrachium rosenbergii compound feed (commercially available) and mixed evenly, and fed to Macrobrachium rosenbergii.

[0023] 2. Determination of non-specific immune indicators of Macrobrachium rosenbergii: after 40 days of continuous feeding of Macrobrachium rosenbergii (the daily feed amount is 3%-7% of the shrimp body weight, subject to eating), the serum of Macrobrachium rosenbergii was extracted, and the shrimp Serum total protein, LZM, ACP, AKP, SOD, POD and PO and other immune indicators were measured. The detection methods of total protein, LZM, ACP, AKP, SOD and POD were operated according to the kit instructions, and the determinatio...

Embodiment 2

[0029] 1. Preparation of compound immune enhancer for Macrobrachium rosenbergii: Tremella polysaccharide 25g, baicalin 10g, tea tree mushroom polysaccharide 35g, levamisole 10g, vitamin C 20g; after mixing evenly, compound immune enhancer was obtained, and 0.1% (accounting for Roche marsh Shrimp compound feed (by weight) is added to Macrobrachium rosenbergii compound feed (commercially available) and mixed evenly, and fed to Macrobrachium rosenbergii.

[0030] 2. Determination of non-specific immune indicators of Macrobrachium rosenbergii: After feeding Macrobrachium rosenbergii for 40 days, extract the serum of Macrobrachium rosenbergii, and measure the immune indicators such as LZM, ACP, AKP, SOD, POD and PO, and the detection method and embodiment 1 is the same.

[0031] 3. Make statistics on the survival rate and weight gain of Macrobrachium rosenbergii.

[0032] The results showed that: the immune indexes LZM, ACP, SOD and PO of Macrobrachium rosenbergii in the experimen...

Embodiment 3

[0036] 1. Macrobrachium rosenbergii is prepared with a compound immune enhancer: Tremella polysaccharide 20g, baicalin 5g, tea tree mushroom polysaccharide 40g, levamisole 5g, vitamin C 30g; after mixing evenly, a compound immune enhancer is obtained, and 0.05% (accounting for Roche marsh Shrimp compound feed (by weight) is added to Macrobrachium rosenbergii compound feed (commercially available) and mixed evenly, and fed to Macrobrachium rosenbergii.

[0037] 2. Determination of non-specific immune indicators of Macrobrachium rosenbergii: After feeding Macrobrachium rosenbergii for 40 days, extract the serum of Macrobrachium rosenbergii, and measure the immune indexes such as LZM, ALP, AKP, SOD, POD and PO, and the detection method and embodiment 1 is the same.

[0038] 3. Make statistics on the survival rate and weight gain of Macrobrachium rosenbergii.

[0039]The results showed that the immune indicators LZM, ACP, SOD and PO of Macrobrachium rosenbergii in the experimenta...

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Abstract

The invention discloses a complex immunity enhancer for Macrobra'chiumrosenbergiideman and an application of the complex immunity enhancer. The complex immunity enhancer for Macrobra'chiumrosenbergiideman is prepared by mixing the following components in percentage by weight: 20-30 percent of tremella polysaccharide, 5-12 percent of baicalin, 30-40 percent of agrocybe cylindracea, 5-13 percent of levomisole and 15-30 percent of vitamin C. The complex immunity enhancer is stable and wide in source of raw materials, low in cost and free of toxic or side effect, can be degraded under a normal condition, has no residual in the Macrobra'chiumrosenbergiideman bodies and can be used for enhancing the non-specific immunity function of the Macrobra'chiumrosenbergiideman and improving the growth capability of the Macrobra'chiumrosenbergiideman.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a compound immune enhancer for Macrobrachium rosenbergii and its application. Background technique [0002] Macrobrachium rosenbergii (Macrobrachium rosenbergii), also known as Malaysian prawns and freshwater long-armed prawns, belongs to Arthropada, Crustacea, Decapoda and Palaemonidae in classification. Macrobrachium (Macrobrachium) is a large freshwater commercial shrimp. Native to tropical and subtropical regions of the Indian Ocean and Pacific Ocean. Macrobrachium rosenbergii was introduced to China from Japan in 1976. After more than 30 years of breeding, especially since the 1990s, with the improvement of artificial breeding technology and the development of breeding technology, the breeding area and production have increased rapidly. By 2009 , my country's annual growth rate of Macrobrachium rosenbergii reaches more than 25 billion, the breeding area is about 30,000 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K20/174A23K20/163
Inventor 林锋沈锦玉刘莉曹铮郝贵杰盛鹏程
Owner ZHEJIANG INST OF FRESH WATER FISHERIES