Skin external preparation composition containing lycium ruthenicum extractive and blackcurrant extractive
A kind of skin external preparation, the technology of black fruit wolfberry, applied in the field of natural product chemistry
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Embodiment 1
[0034] Embodiment 1: the preparation of psammophyte extract
[0035] Dry and pulverize 50g of Lycium barbarum fruit powder, cold-soak with 500ml of 70% ethanol at room temperature, and ultrasonically extract twice, each time for 30min (500W).
[0036] 50 g of blackcurrant fruit dried and pulverized powder was cold-soaked with 500 ml of 70% ethanol at room temperature, ultrasonically extracted twice, each time for 30 min (500 W), the filtrates were combined, and the solvent was recovered under reduced pressure to dryness to obtain the blackcurrant extract.
[0037] The black currant extract and the black currant extract were mixed according to the weight ratio of 10:1, 5:1, 1:1, 1:5 and 1:10 respectively, and prepared into a solution for the corresponding efficacy test.
Embodiment 2
[0038] Example 2: Research on anti-aging effect of psammophyte extract combination
[0039] 1) Research on free radical scavenging
[0040] Principle: According to the DPPH free radical has a single electron, there is a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, the absorption gradually disappears due to the pairing with its single electron, and the degree of fading is similar to that of The number of accepted electrons has a quantitative relationship, so a rapid quantitative analysis can be performed with a spectrophotometer to detect the free radical scavenging situation, so as to judge whether the sample has antioxidant capacity.
[0041] Test method: Add 2ml of the sample solution to be tested with different dilutions and different proportions into the test tube, then add 2ml of 76μmol / L DPPH ethanol solution, mix well, and react in the dark at room temperature for 30min. Absorbance was measured at 525 nm. Each...
Embodiment 3
[0061] Example 3: Research on the whitening effect of psammophyte extract combination
[0062] 1) Study on the inhibitory effect of tyrosinase
[0063] Test method: Add 1ml of sample solutions of different concentrations into the sample tube and sample control tube, and replace the positive and negative control tubes with phosphate buffer. Add 0.5ml tyrosinase solution (enzyme activity unit 125u / ml) in sample tube and positive control tube, replace sample control tube and negative control tube with 0.5ml phosphate buffer solution (pH=6.8), shake all test tubes, make Mix the sample and tyrosinase thoroughly, incubate in a water tank at 37°C for 10 minutes, add 2ml of 0.03wt% tyrosine solution, react for 10 minutes, and measure the absorbance at 475nm immediately.
[0064] Inhibition rate I(%)=[1-(T-T0) / (C-C0)]×100%
[0065] In the formula: T0: sample solvent control; T: sample control; C: positive control; C0: positive control solvent control.
[0066] The test results are s...
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