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Pretreatment method for culturing oleaginous microorganisms from degreased silkworm chrysalis residues, product obtained by using pretreatment method and applications of product

A technology of oil-producing microorganisms and pretreatment, applied in the field of biological resources and the environment, to achieve the effects of mild reaction conditions, improved efficiency, and shortened time

Inactive Publication Date: 2015-01-14
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilize this method to obtain hydrolyzate at the same time, it is used for the cultivation of oleaginous microorganism and has not yet been reported

Method used

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  • Pretreatment method for culturing oleaginous microorganisms from degreased silkworm chrysalis residues, product obtained by using pretreatment method and applications of product
  • Pretreatment method for culturing oleaginous microorganisms from degreased silkworm chrysalis residues, product obtained by using pretreatment method and applications of product

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1 Silkworm chrysalis residue pretreatment

[0023]Weigh 0.5 g of the obtained crude degreased silkworm chrysalis and mix it with 9.5 g of [Bmim]Cl. The ultrasonic power is 150 W, and the reaction temperature is 65 ° C. After 1 h of reaction, elution and centrifugation are performed with a small amount of distilled water several times to remove the ionic liquid. Then add 20mL of water and adjust the pH value to 6.5, then add 0.005g of flavor protease, stir and hydrolyze at 50°C for 60 minutes, and add alkali during the reaction to keep the pH at about 6.5. After the hydrolysis, use a boiling water bath 10min to inactivate the enzyme, centrifuge at 8000r / min for 10min, and take the supernatant. The degree of hydrolysis was measured to be 28.54%.

Embodiment 2

[0024] Embodiment 2 Silkworm chrysalis residue pretreatment

[0025] The thick defatted silkworm chrysalis and 2.8g of [Emim][PF 6 ] mixing, the ultrasonic power is 120W, and the reaction temperature is 60°C. After 1 hour of reaction, elution and centrifugation with a small amount of distilled water are used to remove the ionic liquid. Then add 50mL of water and adjust the pH to 8.0, and add alkali during the reaction to keep the pH at about 8.0, then add 0.015g of trypsin and stir for 90 minutes at 55°C for hydrolysis. After the hydrolysis, use a boiling water bath 10min to inactivate the enzyme, centrifuge at 8000r / min for 10min, and take the supernatant. The degree of hydrolysis was measured to be 26.73%.

Embodiment 3

[0026] Embodiment 3 Silkworm chrysalis residue pretreatment

[0027] The thick defatted silkworm chrysalis and 2g of [Omim][BF 4 ] mixing, the ultrasonic power is 150W, and the reaction temperature is 60°C. After 1 hour of reaction, elution and centrifugation with a small amount of distilled water are used to remove the ionic liquid. Then add 20mL of water and adjust the pH value to 7.0, and add acid or alkali to adjust during the reaction, then add 0.005g of neutral protease and stir at 50°C for 40 minutes for hydrolysis. After the hydrolysis, use a boiling water bath for 10 minutes to treat the enzyme Inactivate, centrifuge at 8000r / min for 10min, and take the supernatant. The degree of hydrolysis was measured to be 23.78%.

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Abstract

The invention relates to a pretreatment method for culturing oleaginous microorganisms from degreased silkworm chrysalis residues, a product obtained by using the pretreatment method and applications of the product. The pretreatment method comprises the following steps: pretreatment of silkworm chrysalis residues with ultrasonic-ionic liquid: mixing the degreased silkworm chrysalis with ionic liquid, eluting with distilled water and centrifuging to remove ionic liquid; preparing the silkworm chrysalis residues with distilled water, adding protease, regulating the pH value of the solution to an optimum pH value of the added protease, adding acid or alkaline in the reaction process so as to maintain adjustment, stirring and hydrolyzing reference to the optimum reaction temperature of the added protease, deactivating enzyme with boiling water bath after the hydrolysis is finished, centrifuging and taking supernatant for later use. According to the pretreatment method, an ultrasonic wave field causes the intensified pretreatment of silkworm chrysalis residues by taking the ionic liquid as a reaction medium, and the protein hydrolysate is prepared by taking protease as a catalyst so as to culturing of oleaginous microorganisms, aiming to improvement of the utilization rate of the silkworm chrysalis resources and increase of grease.

Description

technical field [0001] The invention belongs to the technical field of biological resources and environment, and in particular relates to a pretreatment method for cultivating oil-producing microorganisms with defatted silkworm chrysalis residues, as well as its product and application. Background technique [0002] Traditional fossil fuels, such as oil, coal and natural gas, still dominate globally. In recent years, with the consumption of these traditional non-renewable fuels and their multiple adverse effects on the environment, people have turned their attention to new fuels, and biodiesel has attracted widespread interest from all walks of life because of its carbon neutrality and environmental friendliness. Traditional sources of biodiesel feedstock are mainly vegetable oils (rapeseed oil, corn, soybean, palm oil, coconut). However, if these raw materials are to be continuously supplied, they will inevitably compete with grain for land, and the harvesting period of ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/14C12N1/16
CPCC12N1/12C12N1/14C12N1/16
Inventor 王俊史馨怡李泰莹吴福安张健
Owner JIANGSU UNIV OF SCI & TECH
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