A method for synchronously extracting polysaccharides and proteins from the by-products of Pleurotus eryngii processing

Abstract

The invention relates to a method for synchronously extracting polysaccharides and proteins from by-products of Pleurotus eryngii processing, belonging to the field of intensive processing of agricultural products. The by-products of Pleurotus eryngii processing refers to the part of the roots of the culture medium that has no commercial value and is excised during the processing of mature Pleurotus eryngii mushrooms into fresh mushrooms for sale. Soak the superfine powder of Pleurotus eryngii processing by-products obtained after pretreatment through 85% ethanol at room temperature, centrifuge, add phosphate buffer solution for precipitation, extract protein with salt solution at room temperature, centrifuge, supernatant is ultrafiltered through 100kDa ultrafiltration membrane, The protein was obtained by spray drying, and the retentate was used as a raw material; the polysaccharide was extracted with hot water and centrifuged, and the supernatant was mixed with the above retentate, and the concentrated solution was precipitated by ethanol for 12 hours and centrifuged, and the precipitate was dried at 60°C , dissolved in water, ultrafiltration with 100kDa ultrafiltration membrane, and freeze-drying of the retentate to obtain crude polysaccharides; using hot water extraction, ultrafiltration membrane separation, spray drying, and freeze-drying techniques combined, the polysaccharide yield was 10.69%, and the protein was obtained The rate is 11.23%.

Description

A method for synchronously extracting polysaccharides and proteins from the by-products of Pleurotus eryngii processing 1. Technical field The invention relates to a method for synchronously extracting protein and polysaccharide from by-products of Pleurotus eryngii processing, belonging to the field of deep processing of agricultural products, and the product can be widely used in the fields of functional food, biomedicine and the like. Among them, the by-products of Pleurotus eryngii processing refer to the root pedicle part of the adhesion medium that has no commercial value and is excised during the process of processing the whole mature Pleurotus eryngii into fresh products after harvesting. 2. Background technology Pleurotus eryngii, also known as Pleurotus eryngii, is known as the "king of mushrooms" in the field of edible fungi. Its fruiting body is white in color and crisp and tender in texture. Subphylum, Basidiomycetes, Laminomycetes, Non-separated Basidiomycete...

AI Technical Summary

Problems solved by technology

However, most of the extraction methods for these two types of active substances are carried out separately, and it is difficult to achieve the desired effect under the premise of long extraction time, high extraction temperature, and high energy consumption, which greatly limit the development and utilization of active substances.

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