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A kind of microbial preparation and preparation method for reducing the generation of bottom mud skin

A technology of microbial preparations and mud skins, which is applied in the field of compound microbial preparations and its preparation, can solve problems such as the single ratio of microbial preparations, the cost and utilization of microbial preparations, and irrationality, so as to improve the safety factor of breeding and avoid underwater poisoning , the effect of delaying the impact

Active Publication Date: 2017-08-29
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a single or unreasonable ratio of microbial preparations in the market, which greatly limits the cost and utilization of microbial preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Seed Production

[0041] Inoculate the Bacillus subtilis and Bacillus coagulans purchased from the China Agricultural Microorganism Culture Collection and Management Center respectively in the broth medium, and cultivate them at 37°C for 48 hours. Store in a refrigerator at 4°C until use; inoculate Rhodopseudomonas palustris in the corresponding culture medium, culture for 5 days under light until the color turns deep red, and store in a refrigerator at 4°C for use;

[0042] The second step: single and compound culture of strains

[0043] The strains cultivated in step 1 were weighed according to the following weight percentages: 30% of Bacillus subtilis, 40% of Rhodopseudomonas palustris, and 30% of Bacillus coagulans.

[0044] a: At 28°C, Rhodopseudomonas palustris was inoculated under aseptic conditions in a culture medium sterilized at 120°C for 30 minutes for 5 days, and the light intensity was 5000lx to obtain a photosynthetic bacteria seed culture soluti...

Embodiment 2

[0057] The main evaluation indicators of the experiment of reducing the mud skin at the bottom of the breeding pond are: the change of the mud skin mention, the main water quality index (ammonia nitrogen, nitrite nitrogen, hydrogen sulfide and organic nitrogen) of the mud skin mixture.

[0058] The specific treatment process is as follows: take 1 kg of mud skins at the bottom of the breeding pond, add 8 parts of water respectively to form a mixture solution, one group is added with microbial preparations, and the other group is used as a control, and the treatment time is 7 days.

[0059] After 7 days, both groups of mud-skin mixtures showed obvious mud-skin precipitation, forming a clear solid-liquid boundary. The volume of mud-skin in the group added with microbial preparations was significantly less than that of the control group, and the reduction rate of the drying and weighing comparison reached 66%. %. Moreover, the supernatant of the group added with the microbial prep...

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Abstract

The invention discloses a microbial preparation for reducing production of bottom sludge cake and a preparation method thereof. The microbial preparation is characterized by being prepared from the following raw material: 20-40% of bacillus subtilis, 10-30% of bacillus coagulans and 20-50% of rhodopseudomonas palustris. The preparation method comprises the following steps: respectively inoculating bacillus subtilis, bacillus coagulans and rhodopseudomonas palustris onto corresponding culture media to obtain seed culture solutions; sequentially inoculating each seed culture solution onto a specific culture medium in proportion for fermentation and then centrifuging at a high speed to obtain a high-concentration bacterium solution; transferring the bacterium solution into a matrix and mixing; and finally granulating, drying and pulverizing. According to the microbial preparation, by virtue of a multi-aperture composite carrier structure, a colonization matrix for beneficial bacteria is provided and meanwhile, harmful substances in bottom sludge cake physically adsorbed by the carrier are decomposed by virtue of the beneficial bacteria so that the hardened bottom sludge become soft and decomposed, the buffering capacity of a water body in the entire pond is greatly enhanced so as to constitute a virtuous circle and create a good water-quality environment.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a composite microbial preparation for reducing bottom mud skin production and a preparation method thereof Background technique [0002] With the growth of aquaculture production, blindly pursuing high-density stocking, measures to increase production and efficiency have polluted the aquaculture environment, especially the bottom environment of the aquaculture pond, which has seriously damaged the original ecological balance of the aquaculture pond. The environment of the aquaculture water deteriorates. Once it exceeds the adaptation range of the aquaculture objects, the fish, shrimp and crabs will be in a state of stress, reducing their normal physiological functions, manifesting as anorexia, frailty, susceptibility to disease, and even death. After a large amount of bait feeding in the breeding pond for a period of time, the remaining bait and animal feces will be deposite...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/20C02F3/34C12R1/125C12R1/07
Inventor 江旸陆瑛
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG