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Method for preparing free astaxanthin

A technology of astaxanthin and uniform stirring, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high price, low conversion rate of enzymatic hydrolysis, and difficult separation of products, and achieve the goal of improving the conversion rate Effect

Active Publication Date: 2015-01-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the water insolubility of astaxanthin ester, the low conversion rate of enzymatic hydrolysis, the difficult separation of products, the strong specificity of biological enzymes, and the high price, it is limited in large-scale industrial production and application.

Method used

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  • Method for preparing free astaxanthin
  • Method for preparing free astaxanthin
  • Method for preparing free astaxanthin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Raw materials: 1Kg of Haematococcus pluvialis dry powder (Jingzhou Natural Astaxanthin Factory, 2.0% astaxanthin content) was added to 30kg of water and stirred evenly. The used Haematococcus pluvialis powder contains 38% carbohydrates, 24% fat, 24% protein, 2% ash, 1% minerals, 1% biotin, pigments (including: chlorophyll, carotenoids, etc.) 10 %.

[0029] The astaxanthin in the raw material of Haematococcus pluvialis is analyzed by HPLC-DAD method, and the obtained results are as follows Figure 4 shown, from Figure 4 It can be seen from the figure that the main form of astaxanthin in Haematococcus pluvialis is astaxanthin ester, and astaxanthin monoester is the majority in astaxanthin ester, calculated by the integrated peak area, The relative percentages of free astaxanthin, astaxanthin monoester and astaxanthin diester in Haematococcus were 4.2%, 75.6%, and 20.2%, respectively.

[0030] (2) Ingredients: adding glucose with a mass fraction of 5% to the unifor...

Embodiment 2

[0040] (1) Raw materials: 1Kg of Haematococcus pluvialis (Jingzhou City Natural Astaxanthin Factory, astaxanthin content 2.0%), add water 10 times the weight (W / V) of Haematococcus pluvialls, and stir evenly.

[0041] (2) Ingredients: adding glucose with a mass fraction of 10% to the uniformly stirred mixing system in step (1), stirring uniformly and then sterilizing to obtain the fermentation base material.

[0042] (3) Fermentation: mix the bacterial suspensions of the activated Aspergillus niger and Candida two kinds of bacteria according to the volume ratio of 2:1 to make a mixed bacterial liquid, and then mix the bacterial suspension with 12% The amount of inoculum is inoculated into the sterilized fermentation base material, and then fermented at 40°C for 12 hours, with continuous or intermittent stirring during the process.

[0043] (4) Separation: After the fermentation, adjust the fermented liquid to pH 2.0, rest for 0.5h, centrifuge at 8000r / min, and collect the prec...

Embodiment 3

[0047] (1) Raw material: 10Kg of Haematococcus pluvialis (Jingzhou Natural Astaxanthin Factory, astaxanthin content 2.0%), add 20 times the weight (W / V) of Haematococcus pluvialis, and stir evenly.

[0048] (2) Ingredients: adding glucose with a mass fraction of 8% to the uniformly stirred mixing system in step (1), stirring uniformly, and then sterilizing to obtain a fermentation base material.

[0049] (3) Fermentation: Mix the bacterial suspensions of the activated rhizopus and candida in a volume ratio of 1:1 to make a mixed bacterial suspension, and then inoculate the bacterial suspension with 15% amount, inoculated into the sterilized fermentation base material, and then fermented at 38°C for 20h, with continuous or intermittent stirring during the process.

[0050] (4) Separation: After the fermentation, adjust the fermented liquid to pH 3.0, let it rest for 2 hours, filter, and collect the precipitate.

[0051] (5) Leaching: add the ethanol solution that mass volume r...

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Abstract

The invention discloses a method for preparing free astaxanthin. The method comprises the following steps: fermenting haematococcus pluvialis by virtue of a synergistic effect of rhizopus oryzae, aspergillus niger and candida mycoderma bacteria; and fully using a compound enzyme system which is generated in the fermentation process to convert astaxanthin esters in haematococcus pluvialis into free astaxanthin directionally. In the fermentation process, no acids and alkalis are required in the fermentation process. The process can be used for overcoming the defect that in the process of preparing astaxanthin by using the conventional saponification method, the byproduct astacin is easy to generate and the conversion ratio of astaxanthin prepared by an enzymolysis approach is low, thereby providing a path for green large-scaled production of natural free astaxanthin.

Description

technical field [0001] The invention belongs to the technical field of astaxanthin preparation, and in particular relates to a method for preparing free astaxanthin, that is, a method for preparing free astaxanthin by using Haematococcus pluvialis as raw material through multi-bacteria fermentation. Background technique [0002] Astaxanthin (English Astaxanthin, referred to as ASTA), is a red fat-soluble carotene found in shrimp and crab shells, oysters, salmon, algae, and fungi. It is also the highest level of carotenoid synthesis. Nature's strongest antioxidant ever discovered by man. Recent studies have shown that astaxanthin has many important physiological and biological functions such as anti-oxidation, anti-inflammation, anti-tumor and enhancing immunity, so astaxanthin and its products are loved and valued by consumers, especially those with high The demand for products processed and produced by scientific and technological means is on the rise. [0003] According ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P23/00C12R1/845C12R1/685C12R1/72
Inventor 薛长湖周庆新毛相朝王静凤薛勇王玉明杨鲁
Owner OCEAN UNIV OF CHINA
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