Detection method of content of halohydrocarbon organic substances in shoe-making treatment agent
A detection method, a halogenated hydrocarbon technology, is applied in the field of detection of halogenated hydrocarbon organic content in shoe-making treatment agents, which can solve problems such as unclear and unnoticed toxicity of shoe treatment agents, and achieve strong specificity and high efficiency. The effect of high precision and sensitivity
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Embodiment 1
[0030] 1) Extraction of halogenated hydrocarbons in the treatment agent
[0031] Weigh 1.0g (accurate to 0.1mg) of the treatment agent sample to be tested, add 10mL, 5mL and 5mL of ethyl acetate to the sample in turn for extraction, then mix evenly on a vortex mixer, ultrasonically extract for 30min and centrifuge, Finally, the three extracts were mixed and diluted with ethyl acetate to 25.0 mL for analysis.
[0032] 2) Gas chromatography-mass spectrometry analysis
[0033] Gas chromatography-mass spectrometry was used for analysis, and the chromatographic column was a 5% phenyl / 95% methyl polysiloxane capillary column with a specification of 30m×0.25mm×0.25μm; the temperature of the inlet was 230°C; The bombardment ionization source is 70eV, the ion source temperature is 250°C, the quadrupole temperature is 150°C, and the GC-MS interface temperature is 280°C; the column oven adopts a temperature program, and the initial temperature is 30°C for 12 minutes, and then the temper...
Embodiment 2
[0048] 1) Extraction of halogenated hydrocarbons in the treatment agent
[0049] Weigh 2.0g of the treatment agent sample to be tested (accurate to 0.1mg), add 10mL, 5mL and 5mL of methanol to the sample in turn for extraction, mix uniformly on a vortex mixer for 10min, ultrasonically extract for 30min and centrifuge, and then three The extracts were mixed, diluted with ethyl acetate to 25.0 mL, and the supernatant was taken for analysis.
[0050] 2) Gas chromatography-mass spectrometry analysis
[0051] Using gas chromatography-mass spectrometry, the chromatographic column is a 5% phenyl / 95% methyl polysiloxane capillary column (30m×0.25mm×0.25μm); the temperature of the injection port is 230°C; the electron bombardment ionization source is 70eV , the ion source temperature is 250°C, the quadrupole temperature is 150°C, and the GC-MS interface temperature is 280°C; the column oven adopts a temperature program, and the initial temperature is 30°C for 12 minutes, and then the ...
Embodiment 3
[0060] 1) Extraction of halogenated hydrocarbons in the treatment agent
[0061] Weigh 5g of the sample to be tested (accurate to 0.001mg), add 15mL, 10mL, 5mL, 5mL and 5mL of acetone to extract five times, mix evenly on a vortex mixer for 20min, ultrasonically extract for 30min and centrifuge, and then The five extracts were mixed, diluted with acetone to 50.0mL, and then analyzed.
[0062] 2) Gas chromatography-mass spectrometry analysis
[0063] Using gas chromatography-mass spectrometry, the chromatographic column is a 5% phenyl / 95% methyl polysiloxane capillary column (30m×0.25mm×0.25μm); the temperature of the injection port is 230°C; the electron bombardment ionization source is 70eV , the ion source temperature is 250°C, the quadrupole temperature is 150°C, and the GC-MS interface temperature is 280°C; the column oven adopts a temperature program, and the initial temperature is 30°C for 12 minutes, and then the temperature is raised to 150°C at 10°C / min for 5 minutes,...
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