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A preparation method of a formaldehyde-removing and deodorizing microbial agent

A microbial agent and formaldehyde technology, applied in the field of microbial agent preparation, can solve the problems of harmfulness to human body, unstable performance, and by-products

Active Publication Date: 2017-02-15
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, repeated use of the chemical reaction method will cause new secondary pollution; the physical adsorption method requires power and consumes a lot of energy; the ozone oxidation method is ineffective for inorganic substances and has by-products, and it is harmful to the human body when the ozone concentration is too high; the nanophotocatalytic method Low efficiency and unstable performance; low-temperature plasma method consumes a lot of power and has a short action time, so it needs to be treated irregularly; too many plant purification methods will make the carbon dioxide concentration in the room too high at night, which will affect health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0014] Specific embodiment one: the preparation method of the microbial bacterial agent that removes formaldehyde and deodorizes in this embodiment, carries out according to the following steps:

[0015] 1. After activating Bacillus subtilis HDZK-HJB003, Pseudomonas amygdala HDZK-HJB005 and Alcaligenes faecalis HDZK-HJB012, they were respectively inoculated in beef extract peptone liquid medium, cultured on a shaking table at 37°C for 24 hours, and prepared Bacillus subtilis seed liquid, Pseudomonas amygdala seed liquid and Alcaligenes faecalis seed liquid;

[0016] 2. Mix the Bacillus subtilis seed liquid, the Pseudomonas amygdala seed liquid and the Alcaligenes faecalis seed liquid, inoculate the mixed bacterial liquid in the mixed fermentation medium with an inoculation amount of 10% by volume, and inoculate it at 37°C, 180r Cultivate on a shaker for 3 days under the condition of / min;

[0017] 3. Then centrifuge the cultured bacterial solution at 8000r / min for 10min, take...

specific Embodiment approach 2

[0019] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the Bacillus subtilis HDZK-HJB003 described in step 1 is preserved in the China Center for Typical Culture Collection, the preservation address is Wuhan University, Wuhan, and the preservation date is 2013 On April 9th, the deposit number was CCTCC NO: M2013131; Pseudomonas amygdala HDZK-HJB005 was deposited in the China Center for Type Culture Collection, and the deposit address was Wuhan University, Wuhan, and the deposit date was April 9, 2013. It is CCTCC NO: M2013132; Alcaligenes faecalis HDZK-HJB012 is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, the preservation date is April 9, 2013, and the preservation number is CCTCC NO: M2013133. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the activation method described in step one is to combine Bacillus subtilis HDZK-HJB003, Pseudomonas amygdala HDZK-HJB005 and Alcaligenes faecalis HDZK- HJB012 were respectively inoculated in beef extract peptone slant medium, and cultured at 37°C for 48h. Others are the same as in the first or second embodiment.

[0021] The formula of beef extract peptone slant medium in the present embodiment: 0.15g (NH 4 ) 2 SO 4 , 0.5g KH 2 PO 4 , 0.5gNa 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 2g beef extract, 5g peptone, 0.3g CaCl 2 , 15-20g agar and 1000mL distilled water.

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Abstract

The invention relates to a preparation method of a microbial agent, discloses a preparation method of a microbial agent for removing formaldehyde and odor, and provides a novel method for environmental pollution improvement. The method comprises the following steps: 1 activating bacillus subtilis HDZK-HJB003, almond pseudomonas HDZK-HJB005 and alcaligenes faecalis HDZK-HJB012, then respectively inoculating into a beef extract peptone liquid culture medium, and cultivating to prepare seed solutions; 2 mixing the seed solutions, inoculating mixed bacteria liquid into a mixed fermentation culture medium and cultivating for three days; and 3 centrifuging the cultivated bacteria liquid, taking supernate, and carrying out 4-fold concentration on the supernate, so as to obtain the microbial agent. The microbial agent disclosed by the invention is significant in formaldehyde and odor removal effect, and is applied to removal of formaldehyde and odor in the environment.

Description

technical field [0001] The invention relates to a preparation method of a microbial bacterial agent. Background technique [0002] With the development of society and the enhancement of people's awareness of environmental protection, people put forward higher requirements for environmental quality, and the lawsuits related to environmental pollution are also increasing. Odor substances can not only make people feel unpleasant and disgusting, but also endanger people's health and even life. Therefore, some foreign countries implemented special legislation on odor earlier, and regarded odor as a public nuisance. [0003] The methods and experience of odor pollution control at home and abroad, the common deodorization methods mainly include liquid absorption method, direct combustion or catalytic oxidation method, ozone oxidation method, acid-base absorption method, ionization method, adsorption method, biological method, masking method, photocatalysis Oxidation method, etc. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61L9/01C12R1/125C12R1/38C12R1/05A61L101/52
CPCA61L9/01C12N1/20
Inventor 赵凯王晓华
Owner HEILONGJIANG UNIV