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Determination method of 9 kinds of nutrients in edible vegetable oil

A technology of edible vegetable oil and detection method, which is applied in the field of detection of nutrients in vegetable oil, can solve the problems of cumbersome and complicated operation, long time-consuming, cumbersome operation, etc., and achieve the effects of high sensitivity, shortened detection time and good stability

Active Publication Date: 2015-12-02
ZHANGJIAGANG EENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the current domestic standard method for the determination of sterols GB / T25223-2010 (equivalent to ISO12228: 1999) uses a gas chromatograph (GC-FID) for detection, and its pretreatment includes saponification, extraction, thin layer chromatography, and silanization. These four steps all need to be manually operated, which is cumbersome and complicated to operate, and it is easy to cause problems such as poor repeatability, and it takes about three days to detect with this method, which is extremely time-consuming; in addition, there is an international A kind of high performance liquid chromatography mass spectrometry (HPLC-MS), although thin-layer chromatography and silanization steps have been omitted in the pretreatment process of this method, saponification and extraction are still required, and saponification and extraction still need manual operation, so This method is still very cumbersome and complicated to operate

Method used

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  • Determination method of 9 kinds of nutrients in edible vegetable oil
  • Determination method of 9 kinds of nutrients in edible vegetable oil
  • Determination method of 9 kinds of nutrients in edible vegetable oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Weigh 200mg of mixed extra virgin olive oil sample into a 10mL volumetric flask, dissolve it in a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1, and set the volume to the volumetric line of the volumetric flask. Obtained olive oil solution; 2, the olive oil solution prepared in the first step is injected into the preparative gel permeation chromatograph by the autosampler on the preparative gel permeation chromatograph and purified, and the preparative gel permeation chromatograph is purified. The purification conditions of the gel chromatograph are: a gel chromatographic column (300mm×20mm) fast column, the injection volume is 5mL, the mobile phase is a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1, and the flow rate is 4.7mL / min, the effluent was collected into the receiving tube, and the collection time was from 7'40" to 15'00", and the effluent in the receiving tube was blown dry with nitrogen at 35°C by a f...

Embodiment 2

[0022] 1. Weigh 200 mg of rapeseed oil sample mixed into a 10 mL volumetric flask, dissolve it in a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1, and set the volume to the scale line of the volumetric flask to obtain Rapeseed oil solution; 2. The rapeseed oil solution prepared in the first step is injected into the preparative gel permeation chromatograph by the autosampler on the preparative gel permeation chromatograph for purification. The purification conditions of the gel chromatograph are: the gel chromatographic column is a 300mm × 20mm fast column; the injection volume is 5mL; the mobile phase is a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1; the flow rate is 4.7mL / min; the effluent is collected into the receiving tube, and the collection time is from 7'40" to 15'00", the effluent in the receiving tube is blown dry with nitrogen at 35°C by a fully automatic quantitative concentrator, in order to All the efflue...

Embodiment 3

[0024]1. Weigh 200 mg of soybean oil sample mixed into a 10 mL volumetric flask, dissolve it in a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1, and set the volume to the volumetric line to obtain olive Oil solution; 2. The olive oil solution prepared in the first step is injected into the preparative gel permeation chromatograph by an autosampler on the preparative gel permeation chromatograph for purification, and the preparative gel chromatograph The purification conditions of the instrument are as follows: the gel chromatographic column is a 300mm×20mm fast column; the injection volume is 5mL; the mobile phase is a mixed solvent of cyclohexane and ethyl acetate with a volume ratio of 1:1; the flow rate is 4.7mL / min; The liquid is collected into the receiving tube, and the collection time is from 7'40" to 15'00". The effluent in the receiving tube is blown dry with nitrogen at 35°C by a fully automatic quantitative concentrator. All the effluents...

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Abstract

The invention discloses a method for detecting nine nutrients in edible vegetable oil. The method comprises the following steps: (1) dissolving the edible vegetable oil into a mixed solvent of cyclohexane and ethyl acetate, so as to obtain an edible vegetable oil solution, wherein the volume ratio of cyclohexane to ethyl acetate is 1 to 1; (2) injecting the edible vegetable oil solution prepared in the step (1) into a preparation type gel permeation chromatographic instrument, and dissolving residue of the blow-dried effluent, and filtering to obtain a target analyzing solution, wherein the purification conditions are that a gel chromatographic column is 300mm*20mm, the feeding volume is 5mL, the mixed solvent of cyclohexane and ethyl acetate serves as a moving phase, the flow speed is 4.7mL per minute, the collection time of effluent is from seven forty to fifteen o'clock; and (3) injecting the target analyzing solution obtained in the step (2) into a liquid chromatogram-mass spectrometer, measuring, and analyzing the contents of the nine nutrients, namely erythrodiol, arbutin, ergosterol, brassicasterol, campesterol, fucosterol, stigmasterol, cholesterin and beta-sitosterol by virtue of an external standard method.

Description

technical field [0001] The invention relates to the technical field of nutrient detection in vegetable oil, in particular to a method for detecting nine kinds of nutrients in phytosterols and triterpene diols in edible vegetable oils. Background technique [0002] The main components of edible vegetable oil include triglyceride (95-98%) and unsaponifiable matter (2-5%). The main components in unsaponifiable matter are phytosterols and triterpene diols, and the main nine nutrients in phytosterols and triterpene diols are: erythrodiol, arbutol, ergosterol, brassicasterol, Campesterol, fucosterol, stigmasterol, cholesterol, and β-sitosterol, the nutritional value of these nine nutrients in edible vegetable oils have been generally recognized, and nutritionally fortified vegetable oils with related nutrients have gradually entered the market. [0003] There are many detection methods for phytosterols and triterpene diols in traditional edible vegetable oils. The key step in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 顾强乙小娟石晶刘一军张慧陈忍忍申进玲王玥邵景东
Owner ZHANGJIAGANG EENTRY EXIT INSPECTION & QUARANTINE BUREAU
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