Method for long-term household preservation of individual biological tissue cells and active DNA of individual biological tissue cells

A technology of tissue cells and preservation methods, applied in recombinant DNA technology, DNA preparation, special decorative structures, etc., can solve the problems of DNA pollution, reduce the storage capacity of biological information, disrupt the way of storing DNA molecules, etc., and achieve a simple production process. , good decorative effect, easy to popularize effect

Inactive Publication Date: 2015-02-11
SHANGHAI PAILAIXING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the DNA in the tissue cells is completely preserved in situ, whether it is kept as a souvenir or for future testing, cells with DNA can achieve the same purpose; artificially extracting and storing it is a waste of cost and time. The final free DNA is also easier to degrade and destroy, which increases the difficulty of preservation. Furthermore, the Chelex-100 method in PCR technology is used to extract DNA from cells. With the development of PCR technology, it is no longer necessary to extract DNA. Direct amplification of tissue cells; and, for future DNA analysis technology may not be limited to PCR amplification technology, and this method is for DNA extraction of PCR amplification technology, may not be suitable for new DNA analysi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0029] 1. Reagents needed:

[0030] 1. AA fixative: ethanol-glacial acetic acid solution, components: 95% ethanol and glacial acetic acid are prepared at 99:1;

[0031] 2. Hematoxylin staining solution, eosin staining solution, and neutral gum can all come from commercial products;

[0032] 2. Operation method

[0033] Use the oral test tube to go deep into the oral cavity, wipe the oral mucosa repeatedly, take it out, put it into a preservation bottle containing 2-5 ml of cell preservation solution (commercially available), shake, wash out the cells on the test sample into the liquid, discard the test Transfer to a centrifuge tube at 1000-2000 revolutions / centrifuge for 3-10 minutes, discard the supernatant, add 1 ml of AA fixative, suspend the precipitate and transfer it to a 1.5 ml Eppendorf tube with a disposable dropper, and let it stand for 5-20 Min, 1000-2000 revolutions / separation for 1-5 minutes, discard the supernatant, add 1 ml of distilled water, shake, 1000-2000 revo...

specific Embodiment approach 2

[0034] 1. Reagents needed:

[0035] 1. AFA fixative solution: alcohol-formalin-acetic acid solution, formaldehyde (40%), 95% ethanol and glacial acetic acid at 2:17:1;

[0036] 2. Eosin staining solution and neutral gum can all come from commercial products;

[0037] 2. Operation method

[0038] Use scissors to cut out about 30 cubic millimeters of soft tissue from the surgically removed soft tissue or from the oral mucosa of the biological individual’s remains, put it into a 1.5 ml Eppendorf tube with 1 ml AFA fixative, and let it stand for 15-30 minutes. Aspirate and discard the liquid with a pipette, add 1 ml of distilled water, shake, absorb and discard the liquid with a pipette, add a drop of eosin dye solution, let stand for 30 seconds to 2 minutes, add 1 ml of 95% ethanol, Let stand for 5-20 minutes, suck up the liquid with a pipette and discard, add 1 ml of absolute ethanol, let stand for 5-20 minutes, suck up the liquid with a pipette and discard, then add 1 ml of absolute...

specific Embodiment approach 3

[0039] This embodiment is the same as embodiment 1 or 2, except that the stained cells are put into a glass tube with an inner diameter of 1-5 mm. One end of the tube is blind, and 1-5 drops of natural neutral are added. The resin is embedded, and then the highly transparent unsaturated polyester resin is gently added until it is full and solidified, and then the highly transparent unsaturated polyester resin is wrapped and molded.

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PUM

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Abstract

The invention discloses a preparation method of a souvenir carried with individual biological tissue cells and DNA, and belongs to the field of biotechnology. The organism materials separated from an organism are easy to decay and difficult to store; although a mature method in the biomedical field is provided, a storage mode of the method is unsuitable for household and personal applications. The invention aims to provide a storage method and a preparation process of individual biological cells and a complete genome thereof for household collection. The method disclosed by the invention comprises the following steps of sample collection, fixation, dyeing, dehydration, hyalinizing/sealing, molding and the like. The method has the beneficial effects that the individual biological DNA is completely preserved in a cell nucleus in situ according to a genome unit, i.e., non-renewable human genetic information resources are persistently preserved, so that the biological engineering operation taking a single cell, a single cell nucleus and a single genome as units can be easily carried out in future, and thus an immeasurable value and contribution for future people to research genetics and human evolution is provided.

Description

Technical field [0001] The invention relates to a method for making souvenirs or handicrafts carrying individual biological cells and their DNA, which can be used for households or individuals to permanently store biologically active DNA molecules, belonging to the field of biotechnology. Background technique [0002] Deoxyribonucleic acid contains the genetic information of almost all biological individuals and is one of the greatest discoveries of the 20th century. In the middle of the last century, James Watson and Francis Crick revealed the double helix structure of DNA molecules, put forward the famous central law, laid the foundation of the entire molecular genetics, and created a new era of human understanding of life. Because of this fundamental contribution, the biological sciences have made rapid progress in the past half a century. People can amplify a small amount of DNA of a biological individual millions of times in a short time, and can quickly and low-cost biologi...

Claims

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Application Information

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IPC IPC(8): A44C27/00B44C5/00C12N15/10
Inventor 董建国
Owner SHANGHAI PAILAIXING BIOTECH
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