Method for long-term household preservation of individual biological tissue cells and active DNA of individual biological tissue cells

Abstract

The invention discloses a preparation method of a souvenir carried with individual biological tissue cells and DNA, and belongs to the field of biotechnology. The organism materials separated from an organism are easy to decay and difficult to store; although a mature method in the biomedical field is provided, a storage mode of the method is unsuitable for household and personal applications. The invention aims to provide a storage method and a preparation process of individual biological cells and a complete genome thereof for household collection. The method disclosed by the invention comprises the following steps of sample collection, fixation, dyeing, dehydration, hyalinizing / sealing, molding and the like. The method has the beneficial effects that the individual biological DNA is completely preserved in a cell nucleus in situ according to a genome unit, i.e., non-renewable human genetic information resources are persistently preserved, so that the biological engineering operation taking a single cell, a single cell nucleus and a single genome as units can be easily carried out in future, and thus an immeasurable value and contribution for future people to research genetics and human evolution is provided.

Description

Technical field [0001] The invention relates to a method for making souvenirs or handicrafts carrying individual biological cells and their DNA, which can be used for households or individuals to permanently store biologically active DNA molecules, belonging to the field of biotechnology. Background technique [0002] Deoxyribonucleic acid contains the genetic information of almost all biological individuals and is one of the greatest discoveries of the 20th century. In the middle of the last century, James Watson and Francis Crick revealed the double helix structure of DNA molecules, put forward the famous central law, laid the foundation of the entire molecular genetics, and created a new era of human understanding of life. Because of this fundamental contribution, the biological sciences have made rapid progress in the past half a century. People can amplify a small amount of DNA of a biological individual millions of times in a short time, and can quickly and low-cost biologi...

AI Technical Summary

Problems solved by technology

Because the DNA in the tissue cells is completely preserved in situ, whether it is kept as a souvenir or for future testing, cells with DNA can achieve the same purpose; artificially extracting and storing it is a waste of cost and time. The final free DNA is also easier to degrade and destroy, which increases the difficulty of preservation. Furthermore, the Chelex-100 method in PCR technology is used to extract DNA from cells. With the development of PCR technology, it is no longer necessary to extract DNA. Direct amplification of tissue cells; and, for future DNA analysis technology may not be limited to PCR amplification technology, and this method is for DNA extraction of PCR amplification technology, may not be suitable for new DNA analysis technology in the future

[0009] 3. The original position and original appearance of the genome cannot be maintained, and the integrity is destroyed, disrupting the way DNA molecules are stored in genome units, mixing each set of genomes together, making it impossible to distinguish an independent complete genome, and reducing biological information Storage capacity, not conducive to future detection and cloning of human bodies

[0010] 4. The process of extracting and preparing DNA is complex and cumbersome, and the cost is high, so it is not suitable for automatic operation and popularization

[0011] 5. Easy to contaminate DNA

[0012] 6. The extracted DNA cannot be seen with naked eyes and optical microscopes, and has no visual effects and decoration