A kind of vaccine composition and its preparation method and application

A vaccine composition and antigen technology, applied in the field of vaccine compositions, can solve the problems of the quality decline of the preventive effect, stress and safety problems, etc.

Active Publication Date: 2017-09-05
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] When using vaccines to prevent and treat these three diseases, multiple injections of a single vaccine will cause stress and safety issues, and the quality of the prevention effect will decline

Method used

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  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application
  • A kind of vaccine composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1, SIV, Mhp, APP antigen

[0038] 1. The source of bacteria (virus) strains

[0039] The SIV H1N1ZJS strain was isolated and preserved in our laboratory, the preservation number is CCTCC NO: V201233, and the preservation date is September 11, 2012.

[0040] Mhp HN0613 strain, isolated and preserved in our laboratory, the preservation number is CCTCC NO: M2012230, and the preservation date is June 13, 2012.

[0041] APP1 type LC strain, isolated and preserved in our laboratory, the preservation number is CCTCC NO: M2011458, and the preservation date is December 9, 2011.

[0042] 2. Preparation and inspection of vaccine semi-finished products

[0043] 2.1 Preparation of seeds for production

[0044] 2.1.1 Swine influenza virus

[0045] The SIV H1N1ZJS strain was inoculated into SPF chicken embryos for subculture, and the allantoic fluid was harvested as the virus fluid. The first generation was F1 and then passed to F3. The allantoic flui...

Embodiment 2

[0083] Example 2. Preparation of vaccine composition

[0084] 1 Preparation of preservatives

[0085] 1% (w / v) thimerosal (Sinopharm Chemical Reagent Co., Ltd. 20120106) aqueous solution

[0086] 2 preparation of diluent

[0087] Sterile PBS (0.01M, pH=0.02) buffer solution: dissolve 8g sodium chloride, 0.25g potassium chloride, 3.63g disodium hydrogen phosphate, 0.24g potassium dihydrogen phosphate in 900ml purified water, then dilute to 1L, autoclave at 121°C for 30 minutes for later use.

[0088] 3 preparation of adjuvants

[0089] Gel01 (French SEPPIC company 20120401), autoclaved at 121°C for 30 minutes for later use.

[0090] 4 with seedlings

[0091] Get the concentrated inactivated antigen of Mhp, SS2, SESZ prepared in Example 1, make mixed antigen solution or directly make antigen solution according to the final antigen content in combined vaccine and single vaccine, then antigen solution and Gel01 adjuvant by 90: 10 (v / v) mixed, supplemented to the required vol...

Embodiment 3

[0121] Example 3. This study is to evaluate the promoting effect of mixed infection of SIV, Mhp and APP on the pathogenesis of APP, so as to prove the synergistic effect of mixed infection of the three.

[0122] 1 Material:

[0123] Animals: 60-70 days old, pigs with negative serum antibodies to SIV, Mhp and APP

[0124] Attacking strains: SIV H1N1ZJS strain, Mhp CVCC354 strain (purchased from China Veterinary Drug Administration), APP type 1 LC strain

[0125] 2 methods:

[0126] Fifty piglets aged 60-70 days were selected and randomly divided into 10 groups with 5 pigs in each group. Different doses of APP1 type LC strains were used to challenge pigs in groups 1 to 3, and the incidence was counted after 14 days of observation; the attacking doses of SIV H1N1ZJS strain and different doses of APP type 1 LC strains were used to attack pigs in groups 4 and 5, and observations were made for 14 days. Incidence situation: Use the CVCC354 strain of the pathogenic dose and differe...

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Abstract

The invention provides a vaccine composition, which contains immunological amount of SIV, Mhp, APP antigen and veterinary acceptable carrier. The immunization procedure of the vaccine composition is simple, and can effectively prevent and treat the single infection or mixed infection of SIV, Mhp and APP. When mixed infection occurs, the immune effect is significantly higher than that of single vaccine injections respectively. The vaccine composition has few side effects, long immunization period, less time-consuming and labor-intensive production process, low immunization cost and strong practicability.

Description

technical field [0001] The present invention relates to a vaccine composition. Background technique [0002] Swine influenza (SI) is an acute, febrile and highly contagious respiratory infectious disease caused by swine influenza virus (SIV), characterized by sudden onset, cough, dyspnea, fever and failure, and subsequent rapid recovery or death as a feature. Pigs of all varieties, genders and ages can be infected with a high incidence rate. When influenza breaks out in pig herds, the incidence rate can reach 100%. The typical clinical symptoms of swine flu are runny nose, cough and dyspnea, accompanied by fever, lethargy and subsequent anorexia and weight loss. SIV seldom causes death in the case of single infection, but mixed infection with PRRSV, PCV2 and Mhp will significantly increase the degree of damage and fatality of the above diseases. In addition, SIV is also the main pathogen of porcine respiratory disease syndrome. The damage caused by co-infection or second...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/00A61P31/16A61P11/00
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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