Microalgae culture solution treatment method

A microalgae culture solution and microalgae technology, applied in the field of industrial microbiology, can solve problems such as the difficulty of separating microalgae

Active Publication Date: 2015-02-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the relatively difficult defect of separating microalg

Method used

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preparation Embodiment 1

[0030] Chlorella sorokiniana was cultivated according to the method in the literature (“Effects of different media and compositions on the growth and oil of two types of Chlorella” Hu Huihui, Xu Nianjun, Journal of Biology, 2012.29 (4) 12-16). Chlorella sorokiniana was purchased from ATCC with item number 22521 TM , obtained 4 parts of Chlorella culture solution altogether, the OD value of the first part of Chlorella culture solution was 5.99, and the pH value was 8.69; the OD value of the second part of Chlorella culture solution was 6.09, and the pH value was 8.87; the third part The OD value of the chlorella culture solution was 7.05 and the pH value was 8.31; the OD value of the fourth chlorella culture solution was 6.08 and the pH value was 8.54.

[0031] Cultivate Chlorella vulgaris according to the method in the literature (“Effects of Different Medium and Composition on the Growth and Oil of Two Kinds of Chlorella” Hu Huihui, Xu Nianjun, Journal of Biology, 2012.29 (4)...

Embodiment 1

[0033] At room temperature, take 800 mL of the first chlorella culture solution in Preparation Example 1 with an OD value of 5.99 and a pH value of 8.69, add 1 mol / L aqueous sodium hydroxide solution under stirring at 100 rpm, and adjust the pH value to 11 . Then under stirring at 100rpm, with respect to 1 volume part of microalgae culture solution with adjusted pH value, add 0.005 volume part of flocculant solution, stir for 30 minutes after the addition is completed, and then let stand for 30 minutes to obtain the treated microalgae Algae culture medium.

[0034]Among them, the flocculant solution is formed by dissolving chitosan (purchased from Sinopharm Chemical Reagent Co., Ltd., batch number F20100408, viscosity 50-800mPa·s, N-deacetylation degree 80-95%) and acetic acid in water in sequence An aqueous solution containing 10 mg / L chitosan and 50 mg / L acetic acid.

Embodiment 2

[0036] At room temperature, take 800 mL of the first chlorella culture solution in Preparation Example 1 with an OD value of 5.99 and a pH value of 8.69, and add 1 mol / L aqueous sodium hydroxide solution under stirring at 100 rpm to adjust the pH value to 11.5 . Then under stirring at 100rpm, with respect to 1 volume part of the microalgae culture solution with adjusted pH value, add 0.002 volume part of flocculant solution, stir for 10 minutes after adding, and then stand for 40 minutes to obtain the treated microalgae Algae culture medium.

[0037] Among them, the flocculant solution is formed by dissolving chitosan (purchased from Sinopharm Chemical Reagent Co., Ltd., batch number F20100408, viscosity 50-800mPa·s, N-deacetylation degree 80-95%) and acetic acid in water in sequence An aqueous solution containing 0.1 mg / L chitosan and 100 mg / L oxalic acid.

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Abstract

The present invention provides a microalgae culture solution treatment method, which is characterized by comprising: (1) adjusting the pH value of a microalgae culture solution to be treated to 9.5-12 so as to obtain the microalgae culture solution with the adjusted pH value; and (2) carrying out contact on the microalgae culture solution with the adjusted pH value in the step (1) and a flocculant solution so as to flocculate the microalgae, wherein the flocculant solution contains chitosan and a water-soluble organic acid. With the technical scheme, the flocculation efficiency of the chlorella can be increased to more than 90% by adopting the method of the present invention.

Description

technical field [0001] The invention relates to the field of industrial microbiology, in particular to a method for treating microalgae culture fluid. Background technique [0002] Microalgae have high photosynthetic efficiency, grow rapidly, and can absorb a large amount of carbon dioxide (CO 2 ), effectively reducing the greenhouse effect. Microalgae absorb a large amount of nutrient salts during the growth process, providing a good way for sewage treatment. In particular, the cells of microalgae contain a large amount of fatty acid esters, which are expected to become important basic raw materials for bioenergy. [0003] However, the concentration of microalgae culture solution is low, generally not more than 1%. And the microalgae are in a relatively stable suspension state in the microalgae culture medium, and the microalgae are mostly single-celled organisms, and their diameter is usually between 3-30 μm. The energy consumption is huge; if it is separated by filtra...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/02C12N1/12
Inventor 孙春燕刘伟黄绪耕周旭华荣峻峰
Owner CHINA PETROLEUM & CHEM CORP
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