Preparation method of magnetic nanometer carrier for immobilized enzyme

A technology of magnetic nanocarriers and immobilized enzymes, which is applied in the directions of being immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problems of easy agglomeration of magnetic nanocarriers, harsh preparation conditions, and enzyme activity. Serious loss and other problems, to achieve the effect of high immobilization rate, simplified preparation steps and processes, and good stability

Inactive Publication Date: 2015-02-11
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation conditions are harsh, the steps are complicated, the loss of enzyme activity is serious, the catalytic efficiency is low, the number of cycles is small, and the production efficiency is low. Disadvantages such as low conversion rate

Method used

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  • Preparation method of magnetic nanometer carrier for immobilized enzyme
  • Preparation method of magnetic nanometer carrier for immobilized enzyme
  • Preparation method of magnetic nanometer carrier for immobilized enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take two conical flasks, add 10 mL of deionized water to one of the flasks, pass N 2 After removing the air in the triangular flask and water, add 0.152g FeSO respectively 4 and 0.400gFe 2 (SO4) 3 , after completely dissolving, add 10g CTAB, 20mL isopropanol and 30mL cyclohexane respectively, fully stir until completely dissolved to form stable W / O Fe Fe 2+ / Fe 3+ Inverted microemulsion; add 3.75 mL of deionized water to a separate bottle, pass N 2 After removing the air in the conical flask and the water, add 1.25 mL of 25% ammonia water, 5 g CTAB, 10 mL of isopropanol and 15 mL of cyclohexane, respectively, and stir thoroughly until completely dissolved to form a stable W / O type ammonia water inverse microemulsion; then add 1 mL of / min flow rate, the ammonia water inverse microemulsion was added dropwise to Fe 2+ / Fe 3+ In the reversed-phase microemulsion, after all the additions are completed, continue to stir the reaction for 30 minutes until the reaction is ...

Embodiment 2

[0028] Take two conical flasks, add 10 mL of deionized water to one of the flasks, pass N 2 After removing the air in the triangular flask and water, add 0.260g FeSO respectively 4 ·6H 2 O and 0.562gFe 2 (SO 4 ) 3 ·9H 2 O, after completely dissolving, add 10g CTAB, 20mL isopropanol and 30mL carbon tetrachloride respectively, stir fully until completely dissolved to form stable W / O Fe 2+ / Fe 3+ Inverted microemulsion; add 5.0 mL of deionized water to a separate bottle, pass N 2 After removing the air in the conical flask and the water, add 0.32g NaOH, after dissolving, add 5g CTAB, 10mL isopropanol and 15mL carbon tetrachloride respectively, stir well until completely dissolved to form a stable W / O type NaOH inverse microemulsion ; Then at a flow rate of 1 mL / min, the NaOH inverse microemulsion was added to Fe 2+ / Fe 3+ In the reversed-phase microemulsion, after all the additions are completed, continue to stir the reaction for 30 minutes until the reaction is complete...

Embodiment 3

[0031] Take two conical flasks, add 10 mL of deionized water to one of the flasks, pass N 2 After removing the air in the triangular flask and the water, add 0.186g Fe (NO 3 ) 2 and 0.502gFe(NO 3 ) 3 , after completely dissolving, add 10g CTAB, 20mL isopropanol and 30mL n-heptane respectively, stir fully until completely dissolved to form stable W / O Fe 2+ / Fe 3+ Inverted microemulsion; add 5.0 mL of deionized water to a separate bottle, pass N 2 After removing the air in the conical flask and the water, add 0.448g KOH, after dissolving, add 5g CTAB, 10mL isopropanol and 15mL n-heptane respectively, stir well until completely dissolved to form a stable W / O type KOH inverse microemulsion; At a flow rate of 1 mL / min, KOH inverse microemulsion was added to Fe 2+ / Fe 3+ In the reversed-phase microemulsion, after all the additions are completed, continue to stir the reaction for 30 minutes until the reaction is complete; then add 5 mL of 2.0% (mass fraction) sodium alginate s...

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Abstract

The invention discloses a preparation method of a magnetic nanometer carrier for immobilized enzyme. The carrier takes nano Fe3O4 as a core and alginic acid alkaline-earth metal salt as a shell; the carrier has the characteristics of being small in particle size, uniform in particle, good in monodispersity, large in specific surface area, good in chemical stability, strong in load capacity, good in biocompatibility, strong in magnetic response and the like. The preparation method of the magnetic nanometer carrier comprises the following steps: (1) preparing nano Fe3O4 in a reverse microemulsion system; (2) adding a sodium alginate solution to a reaction system, mixing evenly, and then adding an alkaline-earth metal salt solution; (3) after reaction is ended, obtaining the magnetic nanometer carrier by separation and purification. The preparation method of the magnetic nanometer carrier related to the invention is mild in reaction condition, simple in preparation, and controllable in carrier particle size and pore structure in the preparation process.

Description

technical field [0001] The invention relates to the technical field of enzyme engineering and immobilized enzyme carrier, in particular to a preparation method of an immobilized enzyme carrier. Background technique [0002] Enzymes are biological macromolecules with catalytic function, catalysts for all metabolic reactions in organisms, and basic substances to maintain the life activities of organisms. They have mild catalytic reaction conditions, high catalytic efficiency, strong substrate specificity, and few by-products. It is an ideal catalyst due to its wide application range and convenient adjustment of catalytic activity. With the development of bioengineering, enzyme engineering, molecular biology and other technologies, enzyme preparations have been rapidly developed and widely used in molecular diagnosis, disease prevention, drug preparation, agricultural production, food processing, environmental protection and green chemical synthesis. application. Develop and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10
Inventor 姜建芳
Owner ZUNYI MEDICAL UNIVERSITY
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