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Method for detecting methyl mercury ions

A detection method, the technology of methylmercury, applied in the field of nanotechnology and analysis and detection, can solve problems such as the inability to realize a single identification of methylmercury ion response, and achieve good practical application prospects, high detection sensitivity, and simple operation methods

Inactive Publication Date: 2015-02-11
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is a defect in all these works without exception. It cannot realize a single recognition of methylmercury ions without responding to mercury ions. Methmercury ion

Method used

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  • Method for detecting methyl mercury ions
  • Method for detecting methyl mercury ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of silver nanoparticles with DNA as template:

[0042] Prepare the solution required for the preparation of silver nanoparticles: dilute the T base-rich DNA stock solution consisting of 25 bases with water to obtain a 5 μM / L solution, and prepare 1 mM / L silver nitrate (AgNO 3 ) solution, prepare 10mM / L sodium borohydride (NaBH 4 ) solution, prepare 10mM / L Tris-HNO 3 The buffer solution (pH=7.4) is ready for use;

[0043] Add 490 μL of Tris-HNO to a 1.5 mL centrifuge tube 3 Buffer solution (10mM / L); add 5 μL of the prepared 5 μM / L DNA solution and shake well; take 5 μL of the prepared 1 mM / L silver nitrate solution, add it, shake well, and let it stand for 10 minutes (so that the silver ions and DNA fully function); add 1 μL of 10mM / L sodium borohydride solution for reduction, and shake the centrifuge tube vigorously. Due to the interaction between the silver ion and the base on the DNA and then adsorbed to the DNA, silver nanoparticles with DNA...

Embodiment 2

[0045] Example 2 The quenching effect of silver nanoparticles formed with DNA as a template and free silver nanoparticles on fluorescein fluorescence:

[0046] Take Tris-HNO 3 Add 480 μL of buffer solution (10 mM / L, pH=7.4) into a fluorescent cell with a volume of 1 mL; add 5 μL of fluorescein-labeled T base-rich DNA solution (5 μM / L) into the fluorescent cell, shake well ; Then add 10 μL of different concentrations of silver ion solution or 10 μL Tris-HNO to the fluorescent cell 3 Buffer solution, so that the final concentrations of silver ions are 0, 0.05, 0.2, 0.5, 1.0, 2.0, 4.0, 8.0, 15.0μM, shake well, let stand for 10 minutes; finally add 2μL sodium borohydride solution (10mM / L) to reduce After shaking, place it for 20 minutes, so that the reaction is fully carried out, test and record its fluorescence emission spectrum, and obtain the quenching of the fluorescence of fluorescein by adding different concentrations of silver ions to the DNA template silver nanoparticles....

Embodiment 3

[0049] Example 3 Investigate the ultraviolet-visible absorption spectrum of this detection method to the response mechanism of divalent mercury ions and methylmercury ions:

[0050] (1) Take Tris-HNO 3 Add 480 μL of buffer solution (10 mM / L, pH=7.4) into the absorption pool with a volume of 1 mL; add 5 μL of T base-rich DNA solution (5 μM / L) containing fluorescein labeled into the absorption pool, shake well , to test its absorption spectrum;

[0051] (2) Make three parallel experimental groups: the first group: after adding 5 μ L of silver nitrate solution (1 mM / L) to the solution obtained in step (1), shake it up and let it stand for 10 minutes, then add 1 μ L of sodium borohydride solution ( 10mM / L) for reduction, reacted for 20 minutes, and tested its absorption spectrum; the second group: After adding 5 μL silver nitrate solution (1mM / L) to the solution obtained in step (1), shake it and let it stand for 10 minutes, then add 5 μL Methylmercury ion solution (1mM / L), fina...

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Abstract

The invention discloses a method for detecting methyl mercury ions. With the combination of advantages of a nano material and blocking, caused by the methyl mercury ions, to silver nanoparticles which take T alkali rich DNA marked with fluorescein as a template, retention of fluorescence is achieved, and thus the methyl mercury ions are detected. The method for detecting the methyl mercury ions is convenient, simple and rapid, no expensive instrument is needed, the cost is low, the detection sensitivity is high, the interference of divalent mercury ions is relatively well overcome while a high response to the methyl mercury ions is achieved, and thus the method has good practical application prospect.

Description

technical field [0001] The invention belongs to the field of nanotechnology and analysis and detection, and in particular relates to a method for detecting methylmercury ions. Background technique [0002] Mercury is a heavy metal with strong toxicity, and because of its wide distribution, it is easy to form various forms of pollutants. Among the various forms of mercury compounds, organic mercury, especially methylmercury, is much more toxic to the central nervous system than inorganic mercury because of its strong fat solubility and easy penetration of the blood-brain barrier. It is stable in nature, difficult to metabolize, and easy to carry out prototype accumulation in organisms, making it a highly toxic substance that has attracted much attention ("The Toxicology of Mercury and Its Chemical Compounds", Crit.Rev.Toxicology.2006,36,609- 662). Methylmercury in nature is mostly transformed from inorganic mercury under the action of microorganisms, and can be enriched thr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 杨荣华邓立
Owner HUNAN UNIV
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