Test strip and method for detecting protein by using immunochromatography
A technology for technical detection and immunochromatography, applied in biological testing, measurement devices, analytical materials, etc., can solve the problems of long time, many operation steps, low sensitivity, etc., and achieve the effect of avoiding serious injury
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Embodiment 1
[0042] Embodiment 1, the preparation of the test strip that detects chorionic gonadotropin in the sample by immunochromatography
[0043] figure 1 The indicated test strip preparation method for the detection of chorionic gonadotropin (HCG) by immunochromatographic technique is as follows:
[0044] 1. Carboxyl-modified SiO containing europium complexes 2 The microspheres were provided by Changchun Zhiang Biotechnology Co., Ltd., and the HCG antibody and HCG antigen were provided by Hytest Company of Finland.
[0045] 2. Coupling of fluorescent microspheres and HCG antibody
[0046] Mix 200μl 20mM PBS, pH7.4, fluorescent microspheres with a solid content of 1% and 6 mg carbodiimide, shake at room temperature for 30 minutes, centrifuge at 12,000rpm for 10 minutes, wash twice with borate buffer, and activate the Fluorescent microspheres were resuspended in 300 μl borate buffer. Add 200 μl of 1 mg / ml HCG antibody to the microsphere suspension, shake and react at room temperatu...
Embodiment 2
[0052] Embodiment 2, detect the HCG in the sample with immunochromatography
[0053] Add 100μl of HCG solution containing 0mIU / ml, 1mIU / ml, 2mIU / ml, 5mIU / ml, 10mIU / ml, 20mIU / ml, 100mIU / ml, 200mIU / ml, 1000mIU / ml dropwise to the fluorescent immunochromatography test On the paper strip, after 15 minutes of reaction, use a fluorescence detector (Shanghai Haiyue Plastic Mold Co., Ltd.) to detect the fluorescence signal, and then plot the fluorescence intensity against the HCG concentration to obtain a standard curve of the fluorescence intensity versus the HCG concentration. figure 2 .
[0054] Add 100 μl of HCG-containing human urine dropwise to the sample pad of the fluorescent immunochromatography test strip, and detect the fluorescent signal after reacting for 15 minutes. Combine the fluorescent intensity with the standard curve to calculate the concentration of HCG as 35mIU / ml, and detect it with the ELISA method The results are consistent, indicating that the method of quan...
Embodiment 3
[0056] Embodiment 3, detect the urinary albumin in the sample by immunochromatography
[0057] The test strip preparation method for detecting urinary albumin is the same as in Example 1, wherein the HCG detection antibody is replaced with an albumin detection antibody, the HCG capture antibody is replaced with an albumin capture antibody, the HCG antigen is replaced with an albumin antigen, and the albumin antigen and Antibodies were also provided by Hytest, Finland.
[0058] Add 100μl of albumin solution containing 0mg / L, 1mg / L, 2mg / L, 10mg / L, 20mg / L, 100mg / L, 200mg / L, 1000mg / L dropwise onto the fluorescent immunochromatographic test strips After reacting for 15 minutes, use a fluorescence detector (Shanghai Haiyue Plastic Mold Co., Ltd.) to detect the fluorescence signal, and then plot the fluorescence intensity versus albumin concentration to obtain a standard curve of fluorescence intensity versus albumin concentration.
[0059] Take 100 μl of human urine containing albu...
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