Glycated albumin detection immunochromatography test trip and preparation method thereof

An immunochromatographic test paper and glycosylated albumin technology, which is applied in the field of immunoassay, can solve the problems of unsuitable clinical routine development, high cost, and small sample size, and achieve easy large-scale production, high sensitivity, and simple preparation method Effect

Inactive Publication Date: 2015-02-11
SHENZHEN AIRUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The determination of GA by HPLC can accurately detect the overall level of blood glucose control in patients in the short term, but it was not widely used at that time because of its high cost and small sample size, which was not suitable for routine clinical practice.

Method used

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  • Glycated albumin detection immunochromatography test trip and preparation method thereof
  • Glycated albumin detection immunochromatography test trip and preparation method thereof
  • Glycated albumin detection immunochromatography test trip and preparation method thereof

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preparation example Construction

[0038] A method for preparing an immunochromatographic test strip for detecting glycated albumin, comprising the following steps:

[0039] 1) Preparation of phenylboronic acid markers: Dilute the fluorescent material with buffer, add phenylboronic acid solution, react at 20-25°C for at least 1 hour, use G25 gel column for separation and purification, and collect markers. That is, phenylboronic acid marker;

[0040] 2) Preparation of the sample pad: use cellulose membrane as the solid phase material of the sample pad, soak it in the first phosphate buffer solution with a substance concentration of 0.01-0.3mol / L, and the pH value of the first phosphate buffer solution is 7.2- 7.6, after soaking, dry it to get the sample pad;

[0041] 3) Preparation of the conjugate pad: use glass cellulose membrane as the solid phase material of the conjugate pad, dilute the phenylboronic acid marker with the second phosphate buffer with a concentration of 0.01-0.1mol / L and a pH value of 7.2 ,...

Embodiment 1

[0051] A method for preparing an immunochromatographic test strip for detecting glycated albumin, comprising the following steps:

[0052] 1) Preparation of phenylboronic acid markers:

[0053] Dilute anti-albumin monoclonal antibody and goat anti-mouse IgG antibody to 1mg / ml with sodium bicarbonate-sodium carbonate solution with concentration of 0.1mol / L and pH of 9.6 respectively, take 5ml of antibody solution, respectively Add 30 mg fluorescent material metalloporphyrin solution, stir well, incubate at room temperature for 1 hour, and mix every 15 minutes. Finally, use a G25 gel column for separation and purification to collect the labeled metalloporphyrin-labeled anti-albumin antibody and goat anti-mouse IgG antibody. The first phosphate buffer solution dilution, wherein the first phosphate buffer solution includes polyethylene glycol containing 0.1% by mass percentage, 2.0% bovine serum albumin, and 0.05% first surfactant, sealed with a reagent bottle , Stored at 2-8°C....

Embodiment 2

[0069] Same as Example 1, the difference is:

[0070]In step 1 and step 2, buffer solution is identical with the first phosphate buffer saline component, and its substance concentration is 0.05mol / L, and pH value is 7.4, and it comprises: by mass percentage, 0.01% polyethylene glycol Alcohol, 1% bovine serum albumin and 0.01% primary surfactant.

[0071] In step 3, the second phosphate buffer solution has a substance concentration of 0.5mol / L and a pH value of 7.2, which includes: in terms of mass percentage, 1% bovine serum albumin, 0.1% polyethylene glycol, 0.5% sucrose and 0.01% secondary surfactant.

[0072] The first surfactant and the second surfactant were Triton X-100.

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Abstract

The invention provides a glycated albumin detection immunochromatography test trip and a preparation method thereof. The test trip comprises a liner, an analysis membrane disposed in the middle part of the liner, a water absorption pad arranged at one end of the analysis membrane upper part, a conjugate pad arranged at the other end of the analysis membrane upper part, and a sample pad disposed at one end of the conjugate pad upper part. The analysis membrane is provided with detection lines and a quality control line. The detection lines include a glycated albumin detection line and a hemoglobin detection line. The preparation method comprises: preparation of a phenylboronic acid marker, preparation of the sample pad, preparation of the conjugate pad, preparation of the detection line and quality control line on the analysis membrane, preparation of the water absorption pad and preparation of the glycated albumin detection immunochromatography test trip. The glycated albumin detection immunochromatography test trip provided by the invention can realize quantitative detection of the glycated albumin content in 3-5min only with a trace amount of a whole blood sample, greatly improves the screening speed, and has the advantages of high sensitivity, good specificity and simple structure.

Description

technical field [0001] The invention relates to the technical field of immunodetection, in particular to an immunochromatographic test strip for detecting glycated albumin and a preparation method thereof. Background technique [0002] Blood sugar monitoring is a very important part of the daily diagnosis and treatment of diabetes. Good blood sugar control can effectively delay the occurrence and development of acute and chronic complications of diabetes. In clinical work, it is very important to control the short-term average blood sugar well in order to make the blood sugar reach the standard in an all-round and long-term manner. Glycated albumin (glycated albumin, GA) is one of the indicators of clinical blood glucose monitoring, which has high clinical value in evaluating the recent blood glucose control of diabetic patients and reflecting the changes of blood glucose levels in the short term. [0003] In the human body, glucose and protein undergo a non-enzymatic glyca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531G01N33/532
CPCG01N33/558G01N33/531G01N33/532G01N2800/042
Inventor 谢爱武
Owner SHENZHEN AIRUI BIO TECH
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