Application of 4 gem-Gem-difluoromethylene substituted 3, 5 dyhydroxyl polycarboxylic compound to pest control
A technology of dihydroxy carboxylic acid and gem difluoride, which is applied in the field of pesticides to achieve remarkable technological progress and excellent stomach toxicity
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Embodiment 1
[0020] Example 1 Test of the inhibitory effect of compounds of formula I on JH biosynthesis.
[0021] The present invention adopts the in vitro radiochemical method invented by Professor Tobe et al. to test the activity of all compounds for inhibiting juvenile hormone biosynthesis.
[0022] Test insects and reagents:
[0023] The test insects were five-instar tobacco hornworm moth (Manduca sexta) larvae that had just shed their skin.
[0024] L-[ 14 C-S-methyl]-methionine, purchased from Amersham Company, culture medium M199 (1.3 mM Ca 2+ , 2% Ficoll, without methionine), purchased from GIBCO.
[0025] Experimental steps:
[0026] (1) Microanatomy of CA
[0027] Anesthetize the Manduca sexta moth with ice, place it on a wax plate, open the neck membrane with clock forceps near the head of the insect, take out the CA, and remove the surrounding connective tissue in M199 for bioassay.
[0029] One CA was placed in 100 μL of M199 containing 4...
Embodiment 2
[0037] Example 2 Test of the stomach toxicity effect of the compound of formula I on pests.
[0038] Refer to "Pesticide Indoor Bioassay Test Guidelines Insecticides Part 2: Stomach Toxicity Activity Test-Poisoned Leaf Method" (NY / T 1154.2-2006).
[0039] (1) Test material
[0040] The test insects were the larvae of the second-instar tobacco hornworm (Manduca sexta) that had just shed their skin. Leaf discs were made from young leaves of tobacco (Nicotiana tabacum L.).
[0041] (2) Test method
[0042] Gastric toxicity test was carried out by using the poisoned leaf method, and the mortality rate was checked after 24 hours of drug treatment. As a result, it was found that some of the compounds of formula I had gastric toxicity to Manduca sexta larvae, among which compounds 7, 19 and 20 were the best (Table 2).
[0043] Table 2 Compounds have gastric toxicity to Manduca sexta larvae
[0044]
[0045]
Embodiment 3
[0046] Example 3 Test of the ovicidal effect of the compound of formula I on pests.
[0047] Refer to "Indoor Bioassay Test Guidelines for Pesticides Insecticides Part 5: Immersion Method for Ovicidal Activity Test" (NY / T1154.5-2006).
[0048] (1) Test material
[0049] Fresh eggs of Manduca sexta were collected.
[0050] (2) Test method
[0051] Tested by immersion method. After the eggs were immersed in the medicinal solution for 10 seconds, they were transferred to culture under normal conditions. The corresponding organic solvent treatment without medicament was set as the control. Calculate the hatch inhibition rate. As a result, it was found that some compounds of formula I had ovicidal activity on Manduca sexta larvae, among which compounds 7, 19 and 20 were the best (Table 3).
[0052] Table 3 Compounds have ovicidal effect on Manduca sexta
[0053]
[0054]
PUM
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