Stem cell culture medium
A culture medium and stem cell technology, applied in the biological field, can solve the problems of unstable batch quality, uncertain chemical composition, gene contamination, etc., and achieve the effect of being conducive to large-scale application, low cost and remarkable effect.
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Embodiment 1
[0027] The stem cell medium contains the following components: polyvinyl alcohol, inorganic salts, vitamins, amino acids, glucose, transferrin, insulin, and fibroblast growth factors.
[0028] The polyvinyl alcohol component used as a cell culture medium component is cell culture grade polyvinyl alcohol, which is added in the form of being dissolved in deionized water, and its dosage is 0.1-4.6% in terms of mass concentration, and the molecular weight of the polyvinyl alcohol used is 100000;
[0029] The types and amounts of inorganic salts used as cell culture medium components are as follows: sodium chloride 3 g / L, 4-hydroxyethylpiperazineethanesulfonic acid 5 g / L, sodium selenate 0.0000005 g / L, magnesium sulfate 0.2 g / L , potassium chloride 0.15g / L, sodium phosphate 0.2 g / L, potassium nitrate 0.00009 g / L, calcium chloride 0.08g / L, sodium pyrogluconate 0.05 g / L, sodium hydroxide 0.9 g / L, Sodium carbonate 4.5 g / L;
[0030] The types and amounts of vitamins used as cell cu...
Embodiment 2
[0037] The stem cell medium contains the following components: polyvinylpyrrolidone, inorganic salts, vitamins, amino acids, glucose, transferrin, insulin, and fibroblast growth factors.
[0038] The polyvinylpyrrolidone component used as a cell culture medium component is a cell culture grade, added in the form of being dissolved in a pH buffer solution, and its dosage is 2% by mass concentration, and the molecular weight of the polyvinyl alcohol used is 50,000.
[0039] The types and amounts of inorganic salts used as cell culture medium components are: sodium chloride 5g / L, 4-hydroxyethylpiperazineethanesulfonic acid 7 g / L, sodium selenate 0.000006g / L, magnesium sulfate 0.05 g / L , potassium chloride 0.45 g / L, sodium phosphate 0.075 g / L, potassium nitrate 0.00005 g / L, calcium chloride 0.25 g / L, sodium pyrogluconate 0.4 g / L, sodium hydroxide 0.3 g / L, sodium carbonate 1.5 g / L.
[0040] The types and amounts of vitamins used as cell culture medium components are as follows: ...
Embodiment 3
[0047] The stem cell medium contains the following components: polyethylene glycol, inorganic salts, vitamins, amino acids, glucose, transferrin, insulin, and fibroblast growth factors.
[0048] The polyethylene glycol component used as a cell culture medium component was cell culture grade and added as a liquid in deionized water. Its consumption is 10% by weight concentration, and its molecular weight is 500.
[0049] The types and amounts of inorganic salts used as cell culture medium components are: sodium chloride 4.5 g / L, 4-hydroxyethylpiperazineethanesulfonic acid 5.5 g / L, sodium selenate 0.000020 g / L, magnesium sulfate 0.1 g / L L, potassium chloride 0.25 g / L, sodium phosphate 0.2 g / L, potassium nitrate 0.00006 g / L, calcium chloride 0.11 g / L, sodium pyrogluconate 0.35 g / L, sodium hydroxide 0.6 g / L, carbonic acid Sodium 1.9g / L.
[0050] The types and dosages of vitamins used as cell culture medium components are as follows: vitamin B7 0.000015 g / L, vitamin C dosage 0.00...
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