Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line

Abstract

The invention discloses a breeding and identifying method of a soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line. The method comprises the steps of hybridizing and backcrossing a triticum aestivum-Dasypyrum villosum 5V alien addition line DA5V and a Chinese spring ph1b1b mutant, identifying individual plants relevant to 5VS and ph1b1bph1b1b from BC1F1 by use of a molecular marker, identifying individual selfed seeds by use of GISH (genomic in situ hybridization) technology, and analyzing individual plants containing translocated chromosomes by use of C-zoning, GISH and molecular markers to prove that the translocated chromosome is T5VS.5AL. A PCR (polymerase chain reaction) primer is designed by use of an EST (Expressed Sequence Tag) located on the homologous chromosome group of the fifth part of triticum, a co-dominant marker capable of identifying the translocated chromosome specially can be screened, and homozygosis and heterozygosis translocations can be distinguished. The breeding utilization value of the translocation line can be disclosed through agronomic traits, kernel hardness analysis and powdery mildew resistance identification.

Description

technical field [0001] The patent of the invention discloses a soft, powdery mildew-resistant common wheat-T5VS·5AL translocation line NAU421 breeding and identification method, which belongs to the technical field of plant breeding. Background technique [0002] Dasypyrum villosum (L.) Schur (2n=2x=14, VV) is a diploid relative species of common wheat native to the northeast of the Mediterranean coast and Southwest Asia. It is an important tertiary gene resource for wheat variety improvement (see reference: Agnieszka Gradzielewska. The genus Dasypyrum-part 2. Dasypyrum villosum-a wild species used in wheat improvement. Euphytica, 2006, 152:441-454) . The Cell Institute of Nanjing Agricultural University used the Triticum villosa 91C43 introduced from the Cambridge Botanical Garden as a donor, and bred a set of common wheat-Multicella villosa heteroaddition lines (see references: Zhang W, Zhang RQ, Feng YG, Bie TD , Chen PD. Distribution of highly repeated DNA sequences in...

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Problems solved by technology

In addition, the practice of powdery mildew resistance breeding in China in recent decades shows that the breeding work is always passively catching up with the changes in the physiological races of pathogenic bacteria. The resistance is obviously lost, and some varieties even lose their resistance before they are approved.

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