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Method for establishing non-alcoholic fatty liver disease combined with viral hepatitis mouse model

A technology for fatty liver disease and viral hepatitis, which is applied in the field of biomedical research to achieve the effects of low cost, complete virus infection and replication cycle, and prevention of deterioration

Inactive Publication Date: 2015-02-25
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for establishing a mouse model of NAFLD combined with viral hepatitis in view of the deficiencies in the existing NAFLD combined with viral hepatitis animal model. The mouse model established by this method overcomes the existing NAFLD combined with virus Due to the shortcomings of the animal model of chronic hepatitis, the model mouse is cheap to construct, easy to reproduce, has obvious clinical symptoms, has a complete virus infection and replication cycle, and can stimulate the immune system to generate an antiviral immune response, and can replicate the NAFLD combined virus Multiple features of chronic hepatitis and similar to the human situation

Method used

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  • Method for establishing non-alcoholic fatty liver disease combined with viral hepatitis mouse model
  • Method for establishing non-alcoholic fatty liver disease combined with viral hepatitis mouse model
  • Method for establishing non-alcoholic fatty liver disease combined with viral hepatitis mouse model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. High-fat diet induction treatment

[0062] 1A) Select 125 SPF female C3H / HeN mice aged 6-7 weeks and feed them with high-fat diet (referred to as high-fat diet group mice). The high-fat diet was purchased from Nantong Trophy Feed Technology Co., Ltd. , product number TP23520, each kilogram of feed contains 258g casein, 162g dextrin, 89g sucrose, 32g soybean oil, 317g lard, 65g cellulose, 58g minerals, 13g vitamins, 4gL-cystine, 3g choline bitartrate and 0.069g food antioxidants;

[0063] In the present invention, the experimental mice are selected as docile female mice, which is not only convenient for raising but also beneficial for the smooth progress of the test treatment.

[0064] 1B) Take another 15 SPF grade female C3H / HeN mice aged 6-7 weeks and feed them with normal diet (abbreviated as normal control group mice);

[0065] 1C) The weights of the two groups of mice were weighed every week, and the general conditions of the mice were observed. After 12 weeks ...

Embodiment 2

[0094] Except step 2B) set the titer to 1.0×10 6 PFU / ml of murine hepatitis type 3 virus was diluted to 5 PFU / 100 μl with sterile PBS buffer; in step 2C), each mouse was injected with 5 PFU of murine hepatitis type 3 virus, and the others were the same as in Example 1.

[0095] 20 infected mice were randomly selected to observe the state and survival rate of the mice. The mice showed symptoms such as listlessness, erect hair and dullness, curled up and trembling, and sluggishness within 5-15 days of virus infection; 4 mice were infected Death occurred on the 6th-13th day, and the survival rate was 80%; after 15 days of infection, the mice did not die again;

[0096] After the mice were infected with murine hepatitis type 3 virus, the peripheral blood of the mice was collected on the 4th day, 8th day, 12th day, 16th day, 20th day, and 30th day to detect transaminase, and the liver tissue was collected for hematoxylin-eosin staining. The peripheral blood and liver tissue of mic...

Embodiment 3

[0101] Except step 2B) set the titer to 1.0×10 6 The pFu / ml murine hepatitis type 3 virus was diluted to 15 PFU / 100 μl with sterile PBS buffer; step 2C) except that 15 PFU of murine hepatitis type 3 virus was injected into each mouse, the rest were the same as in Example 1.

[0102] 20 infected mice were randomly selected to observe the state and survival rate of the mice. The mice had symptoms such as listlessness, erect hair and dullness, curled up and trembling, and slow movement within 3-15 days of virus infection; 7 mice were infected Death occurred on the 3rd to 12th day, and the survival rate was 65%. After 15 days of infection, the symptoms of the mice were gradually relieved, and no death occurred again;

[0103] After the mice were infected with murine hepatitis type 3 virus, the peripheral blood of the mice was collected on the 4th day, 8th day, 12th day, 16th day, 20th day, and 30th day to detect transaminase, and the liver tissue was collected for hematoxylin-eosi...

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Abstract

The invention discloses a method for establishing a non-alcoholic fatty liver disease combined with viral hepatitis mouse model. The method comprises the steps that high-fat feed is used for feeding a C3H / HeN small mouse for 12 weeks, the body weight, the liver weight, transaminase, the glucolipid metabolism index and liver histological change of the small mouse are observed, and a non-alcoholic fatty liver disease small mouse is cultivated. Then 10 PFU of MHV-3 infected mice are selected, and the survival rate, the liver histology and intrahepatic virus replication of the infected mice are observed, so that the non-alcoholic fatty liver disease combined with viral hepatitis mouse model similar to a human disease process is established. The established model provides a forceful tool for virus dynamics, immunological changes and prevention and control measures systematically studying non-alcoholic fatty liver disease combined with viral hepatitis.

Description

technical field [0001] The invention belongs to the field of biomedical research, and relates to a method for constructing an animal model, in particular to a method for constructing a mouse model for simulating, researching and preventing and treating nonalcoholic fatty liver disease combined with viral hepatitis. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is the manifestation of metabolic syndrome in the liver, including simple fatty liver (simple steatosis), steatohepatitis (Non-alcoholic steatohepatitis, NASH), and NASH-related Liver fibrosis / cirrhosis, liver cancer. With the improvement of living standards, the incidence of NAFLD in developing countries has increased significantly. The incidence of NAFLD in my country has nearly doubled in the past 10 years, and the incidence in some areas has reached a quarter. my country is an area with a high incidence of hepatitis virus infection. There are about 100 million hepatitis B virus (Hepatitis...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K35/76A23K1/18A23K1/16
CPCA01K67/02A61K35/76A23K20/158A23K50/50
Inventor 宁琴罗小平习东吴婷王宏武王晓晶严伟明
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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