A kind of sabin strain poliovirus type Ⅱ monoclonal antibody and its application

Abstract

The invention provides a Sabin strain poliovirus type II monoclonal antibody and an application thereof, and belongs to the immunology field. After a mouse is immunized and inoculated with Sabin strain poliovirus type II, mouse spleen cells are fused with mouse myeloma cells, a hybridoma cell strain producing the anti-Sabin strain poliovirus type II specific monoclonal antibody is screened and has the preservation number of CGMCC No.9232, and the secreted monoclonal antibody has high titer, has strong neutralizing activity, and can effectively block infection of the poliovirus type II; at the same time, the antibody can specifically distinguish the poliovirus type II from poliovirus type I, poliovirus type III and other various enteroviruses, and can be used for preparing type II antigen content detection kits and antibody detection kits of the poliovirus type II and a poliomyelitis inactivated vaccine, also can be used for identification detection of the poliovirus type II, and has the broad application prospect.

Description

technical field [0001] The invention relates to the fields of immunology and vaccinology, in particular to a neutralizing monoclonal antibody against Sabin strain poliovirus type II, a hybridoma cell line producing the antibody and the application of the antibody. Background technique [0002] Poliomyelitis disease existed in human society as early as 1580-1350 BC. The earliest evidence comes from a figure in a standing posture in ancient Egyptian stone murals, with obvious symptoms of poliomyelitis disease: bent and atrophied legs, Deformity of the top of the foot. However, it was not until 1789 that Englishman Michael Underwood first described the clinical symptoms of polio disease, which he called "weakness of the lower extremities". Half a century later, research by the Germans showed that polio disease could be contagious. In 1894, the largest catastrophe of paralyzing infants on record occurred in the United States, which was later confirmed to be a disease of polio....

AI Technical Summary

Problems solved by technology

[0005] Due to the polyantibody coating-polyantibody sandwich detection system commonly used in the detection of poliovirus type Ⅱ virus antigens, this system has a large cross-reaction for types Ⅰ and Ⅲ, especially when it is used for the Sabin strain of the type 3 mixed vaccine. When detecting IPV type II antigen, the test results cannot truly reflect the content of type II antigen and the immunogenicity of vaccine type II due to cross-reaction. At the same time, WHO also recommends the matching method of polyclonal antibody and monoclonal antibody to detect trivalent polio vaccine The content of various antigens in the test; at present, there is still a lack of standardized reagents for the detection of Sabin strain IPV D antigen in the world, and the preparation of monoclonal antibodies with good specificity and neutralizing activity can lay the foundation for the standardization of Sabin strain IPV in vitro potency detection methods

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