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Preparation method of high-purity sulforaphen

A sulforaphane, high-purity technology, applied in fermentation and other directions, can solve the problems of unsuitability for industrial production, difficult regeneration of column chromatography materials, complicated production process, etc., and achieves the effects of high cost, long extraction time and simple preparation.

Active Publication Date: 2015-02-25
安徽中鸣科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production process of this process is complex, the consumption of organic reagents such as acetone, n-hexane and methanol is large and there is certain toxicity. The silica gel column developer uses toxic reagents for elution, and there are problems such as flammability and explosion during the production process. Column chromatography It is difficult to regenerate materials and is not suitable for industrial production

Method used

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  • Preparation method of high-purity sulforaphen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Weigh 1 kg of washed red radish, add 200 mL of water, and mash it with a tissue masher to obtain radish juice;

[0023] 2) Grind 100 g of dried broccoli seeds through a 60-mesh sieve, add 200 mL of radish juice prepared in step 1) and 0.1 g of V C Catalyzed enzymolysis at room temperature for 30 minutes to obtain a solid-liquid mixture;

[0024] 3) Add 2000mL of water to the solid-liquid mixture obtained in step 2), mix well, extract with 40MPa high-pressure homogeneous circulation, and collect the filtrate by filtration;

[0025] 4) Add 2000mL ionic liquid [BMIM][PF to the filtrate obtained in step 3) 6 ] extracted 3 times, combined the organic phase, added 2000mL absolute ethanol to the organic phase to back-extract the sulforaphane, and reclaimed the ionic liquid;

[0026] 5) Concentrating the mixture of sulforaphane and ethanol obtained in step 4) back-extracted in vacuo to an oily extract to obtain the sulforaphane extract. Weigh 5 mg of oily extract and add ...

Embodiment 2

[0028] 1) Weigh 1 kg of washed carrots, add 400 mL of water, and mash with a tissue masher to obtain radish juice;

[0029] 2) Grind 300g of dried broccoli seeds through a 60-mesh sieve, add 400mL of radish juice prepared in step 1) and 0.3g of V C Catalyzed enzymolysis at room temperature for 50 minutes to obtain a solid-liquid mixture;

[0030] 3) Add 4000mL of water to the solid-liquid mixture obtained in step 2), mix well, extract with 60MPa high-pressure homogeneous circulation, and collect the filtrate by filtration;

[0031] 4) Add 3000mL ionic liquid [BMIM][PF to the filtrate obtained in step 3) 6 ] extracted 3 times, combined the organic phase, added 3000mL absolute ethanol to the organic phase to back-extract the sulforaphane, and reclaimed the ionic liquid;

[0032] 5) Concentrating the mixture of sulforaphane and ethanol obtained in step 4) back-extracted in vacuo to an oily extract to obtain the sulforaphane extract. Weigh 5 mg of oily extract and add 10 mL of ...

Embodiment 3

[0034] 1) Weigh 1 kg of washed white radish, add 300 mL of water, and mash it with a tissue masher to obtain radish juice;

[0035] 2) Grind 200 g of dried broccoli seeds through a 60-mesh sieve, add 300 mL of radish juice prepared in step 1) and 0.2 g of V C Catalyzed enzymolysis at room temperature for 40 minutes to obtain a solid-liquid mixture;

[0036] 3) Add 3000mL of water to the solid-liquid mixture obtained in step 2), mix well, extract with 50MPa high-pressure homogeneous circulation, and collect the filtrate by filtration;

[0037] 4) Add 4000mL ionic liquid [BMIM][PF to the filtrate obtained in step 3)6 ] extracted 3 times, combined the organic phase, added 3000mL absolute ethanol to the organic phase to back-extract the sulforaphane, and reclaimed the ionic liquid;

[0038] 5) Concentrating the mixture of sulforaphane and ethanol obtained in step 4) back-extracted in vacuo to an oily extract to obtain the sulforaphane extract. Weigh 5 mg of oily extract and add ...

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Abstract

The invention discloses a preparation method of high-purity sulforaphen. The preparation method comprises the steps of crushing broccoli seeds or broccoli bulbs, and performing enzymolysis, high-pressure homogenization, filtration, ionic liquid extraction and the like to obtain the high-purity sulforaphen. The method disclosed by the invention is simple in steps and easy in operation; the content of sulfuric myrosinase is increased by adding carrot juice, the activity of myrosinase is significantly improved under VC (vitamin) catalysis, and the conversion rate of the sulforaphen is improved; the extraction rate of the sulforaphen is improved and the extraction time is shortened by high-pressure homogenization extraction; the extraction efficiency is improved by ionic liquid extraction; and the method is economic and environment-friendly, the final purity of the sulforaphen is more than 95%, the whole process time is not more than 4 hours, the production efficiency is improved, and the whole extraction process does not need other organic solvents, so the method is pollution-free, realizes low-temperature operation and belongs to a typical environment-friendly process.

Description

1. Technical field [0001] The invention relates to an extraction and purification method, in particular to a preparation method of high-purity sulforaphane, which belongs to the field of deep processing of agricultural products. 2. Background technology [0002] Sulforaphane is one of the natural products with the strongest anti-cancer and anti-cancer ability found in vegetables so far. It has obvious blocking effect on a variety of cancer cells, and has pharmacological properties such as antibacterial, improving the body's antioxidant capacity and enhancing immunity. It has no obvious adverse reaction to the human body, has the potential to be developed into a new type of anticancer drug or health care product, and has a broad market prospect. [0003] The pharmacological activity of chemically synthesized sulforaphane is obviously low. At present, sulforaphane mainly comes from broccoli seeds. Most of the existing methods are to directly extract sulforaphane precursors fr...

Claims

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Application Information

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IPC IPC(8): C12P13/00
Inventor 陈彦杨丹刘柳王亚徐利陆永明张亚萍
Owner 安徽中鸣科技有限公司
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