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A kind of method for rapid multiplication of ground pepper

A fast and ground pepper technology, applied in the agricultural field, can solve the problems of high survival rate and subculture variation, and achieve the effects of low variation rate, increased light intensity, and maintenance of genetic stability

Inactive Publication Date: 2016-11-30
湖北云天花海旅游股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing problems at present, the present invention provides a method for rapid multiplication of ground pepper, which can quickly and effectively propagate ground pepper, and solve the problem of subculture variation. Multiplication costs to meet the needs of large-scale production

Method used

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  • A kind of method for rapid multiplication of ground pepper

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: a kind of method for ground pepper rapid multiplication, comprising

[0036] 1. Selection of explants: at the end of June, at the full flowering stage, take the anthers at the single-nuclei marginal stage at 9:00-10:00 in the morning.

[0037] 2. Disinfection treatment: Wrap the anthers with sterile gauze and immerse them in 70-75% alcohol for disinfection for 8-16 seconds, take them out quickly and immediately soak them in bleaching powder for 10 minutes, and finally rinse them with sterile water for 4-6 times.

[0038] 3. Inoculation culture: inoculate the sterilized explants into the callus induction medium, and culture to obtain the callus. The callus induction medium is: 1 / 2MS+6-BA1.2mg / L+NAA 0.12mg / L+sucrose 50g / L; the pH of the medium is 6.6, the culture temperature is 25±1℃, and the light is 12h / d Period, the light intensity is about 1700Lx.

[0039] 4. Subculture proliferation culture, transfer the non-polluted callus tissue into the first subcu...

Embodiment 2

[0047] Embodiment 2: a kind of method for rapid multiplication of ground pepper, comprising

[0048] In addition to the subculture process, the uncontaminated callus was transferred to the first subculture medium to proliferate the shoots. After four subcultures, it was transferred to the blank medium for one subculture, and then transferred to the first subculture medium. Proliferation continued in the substitute medium, wherein the blank medium was 1 / 2MS+potassium dihydrogen phosphate 150mg / L+hydrolyzed milk protein 500mg / L+sucrose 30g / L, and all the other were the same as in Example 1.

Embodiment 3

[0050] Except that "the rooting medium is: 1 / 2MS+IAA 0.1mg / L+sucrose 20g / L+agar 3g / L+activated carbon mass ratio 0.3%", the rest are the same as in Example 1.

[0051] Any combination of the above conditions can achieve the same effect.

[0052] The cultivation results of the present invention are as follows: the callus induction rate reaches 89%, the rooting rate reaches 85%, the transplanting survival rate reaches 80%, and the 10-generation variation rate is below 6%.

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Abstract

The invention discloses a rapid mongollian thyme herb proliferation method. The method comprises the following steps: carrying out disinfection treatment to anthers in the late uninucleate stage; inoculating in a callus-inducing medium and culturing to obtain a callus; carrying out multiplication culture, transferring the non-contaminated callus into a first subculture medium for bud proliferation, subculturing for 4 times, transferring into a second subculture medium or a blank culture medium without any hormones for subculturing for one time, then transferring into the first subculture medium for further multiplication, and repeating the steps according to the required seedling amount; inoculating in a rooting medium, and screening to obtain an individual plant. The culture results are that the induction rate of the callus is up to 89%, the rooting rate is up to 85%, the transplanting survival rate can reach 80% and the 10-generation aberration rate can be below 6%.

Description

technical field [0001] The invention belongs to the field of agricultural technology, relates to tissue culture technology, in particular to a method for rapidly multiplying ground pepper. Background technique [0002] Ground pepper, also known as ground pepper ("Famous Prescriptions on the Sea"), Thyme ("Dictionary of Botany"), Sanjiao ("Chinese Medicinal Plant Illustrated Book"), and mountain pepper ("Liaoning Economic Flora"). For Labiatae plants. Mainly distributed in Inner Mongolia, Ningxia, Qinghai, Shanxi and other places. In the application of traditional Chinese medicine, it can dispel wind and relieve the exterior, promote qi and relieve pain, and is used for colds, headaches, toothaches, abdominal distension and cold pain. [0003] The whole plant of ground pepper has a strong fragrance. Pick a few leaves and rub them in your hands to emit a faint fragrance, which is mild and pleasant. Aromatic oil accounts for 3% of the stem tip and leaves, and the main compon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 徐肖龚晨航潘健
Owner 湖北云天花海旅游股份有限公司
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