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Lactobacillus fermentum strain Lee working starter product and its use for preventing constipation

A technology of working starter, Lactobacillus fermentum, applied in the field of microbiology

Inactive Publication Date: 2017-07-11
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents or patent applications do not fully relate to microorganisms that have the properties of regulating intestinal motility, preventing constipation, etc.

Method used

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  • Lactobacillus fermentum strain Lee working starter product and its use for preventing constipation
  • Lactobacillus fermentum strain Lee working starter product and its use for preventing constipation
  • Lactobacillus fermentum strain Lee working starter product and its use for preventing constipation

Examples

Experimental program
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Effect test

preparation example Construction

[0036] According to the preparation method of the above-mentioned lactic acid bacteria milk beverage, only the raw milk is heat-sterilized at 95°C for 20 minutes or at 140°C for 2 seconds, then cooled to 37°C, and then inoculated with 4% of the volume of the raw milk. The above-mentioned Lactobacillus fermentum strain Lee working starter was fermented at 37°C for 16 hours to obtain Lactobacillus fermentum strain Lee fermented milk; V: V) was added to the above-mentioned sterilized raw milk, homogenized, vacuum concentrated, and spray-dried to obtain milk powder containing Lactobacillus fermentum strain Lee.

[0037] The homogenization is, for example, carried out using a GJB500-40 small homogenizer sold by Changzhou Homogenization Machinery Co., Ltd.

[0038] The concentration is, for example, carried out using a vacuum concentration pot sold by Shanghai Weizhou Light Industry Machinery Co., Ltd.

[0039] The spray drying is carried out, for example, by using an experimental ...

Embodiment 1

[0045] Example 1: Isolation, purification and preliminary identification of Lactobacillus fermentum strain Lee

[0046] This embodiment is carried out according to the following steps:

[0047] (1) Separation and purification of Lactobacillus fermentum strain Lee

[0048] Aseptically draw 500 μL of the sample and add it to 5 mL of sterilized saline to make a uniform dilution of 1:10, and continue to make a certain proportion of dilution. Select the appropriate gradient diluent, draw 100 μL each into the MRS solid plate medium with a sterile pipette tip, and incubate at 30°C for 48-72h, observe and record the colony morphology ( figure 1 ). Use an inoculation loop (or a sterilized toothpick) to pick different colonies from the surface and inside of the plate and inoculate them in MRS liquid medium, culture them on a 300r shaker at 30°C for 24-48 hours; Ran's staining microscope ( figure 2 ), determined to be the continued activation of G+ bacteria until pure colonies were ...

Embodiment 2

[0053] Embodiment 2: In vitro screening of Lactobacillus fermentum strain Lee

[0054] (1) Screening of probiotics resistant to pH 3.0 artificial gastric juice

[0055] Preparation of artificial gastric juice: 0.2% NaCl, 0.35% pepsin, adjust the pH value to 3.0 with 1M HCl, filter and sterilize with a vacuum pump in an aseptic operating table for later use.

[0056] Determination of the tolerance of probiotics to artificial gastric juice: take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operating table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL of sterilized Mix normal saline to make bacterial suspension, mix 1mL bacterial suspension with 9mL pH3.0 artificial gastric juice, shake well, place in a constant temperature shaker (37°C, 300r), and take samples at 0h and 3h respectively, Incubate at 37°C for 48h with MRS agar medium. Measure the number of viable bacteria with the plate counti...

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Abstract

The invention discloses a lactobacillus fermentum Lee working fermenting agent product and application thereof to prevent constipation and keep healthy. Lactobacillus fermentum Lee is preserved in China Center for Type Culture Collection with the preservation number of CCTCCM2013512. Lactobacillus fermentum Lee is capable of normally growing in human intestinal tracts. Mouse in-vivo tests show that lactobacillus fermentum Lee is capable of alleviating the mouse body weight mitigating trend caused by constipation, accelerating melaena discharging time, improving intestinal propulsive rate, increasing levels of MTL, Gas, ET, AchE, SP and VIP in serum to different degrees, reducing SS factor level, increasing food intake and water intake, and reducing the decrease trend of defecation amount, defecation particle quantity and water content in faeces, gives play to effects similar to that of constipation-treating medicines, and shows certain constipation prevention effect. The optimum usage dosage is 1.0*10<9> CFU for per kg body weight.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, more specifically, the present invention relates to a kind of Lactobacillus fermentum that can regulate intestinal movement and prevent constipation and use the bacterial strain as a starter Or added fermented milk, health food and animal health products. Background technique [0002] Lactobacillus is closely related to human life and is one of the beneficial microorganisms widely used in food fermentation, industrial lactic acid fermentation and medical care. Many of the now recognized Lactobacillus and Bifidobacterium genera have specific favorable properties and genetic traits, showing extremely strong probiotic properties. Lactobacillus can not only improve the nutritional value of food, improve the flavor of food, prolong the storage time, but also improve the functional properties of food. It can improve the digestibility and biological value of food and promote digestion a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C9/123A23C9/127A23L33/135A23K10/18
Inventor 李键索化夷骞宇赵欣兰道亮陈炼红谢婕
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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