One-stop preparation method of vascularized life structure

A structure and vascularization technology, applied in the direction of prosthesis, medical science, etc., can solve the problem of inability to solve the problem of cell positioning, and achieve the effect of improving the survival rate of implantation, reducing the volume of materials, and promoting integration.

Active Publication Date: 2017-03-15
上海蓝衍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, for the cell assembly that is crucial to living structures, the current technologies are mainly laser technology and extrusion / jet technology, but these methods cannot solve the problem of cell positioning inside the microstructure

Method used

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  • One-stop preparation method of vascularized life structure
  • One-stop preparation method of vascularized life structure
  • One-stop preparation method of vascularized life structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] see Figure 1~Figure 6 , the one-stop preparation method of the vascularized life structure: the preparation system uses the syringe pump (1) to provide the driving force required for material extrusion; two medical piston syringes are used as the two supply syringes (2, 3), One is the core supply syringe (3), the other is the shell supply syringe (2); the inner needle of the coaxial nozzle (4) is connected to the core supply syringe (3), the coaxial nozzle (4 ) is connected to the shell feeding syringe (2); the preparation process is as follows:

[0031] 1) Stock solution preparation: the biological material and its cross-linking agent are prepared in a set ratio with the corresponding solvent to prepare the biological material stock solution and the cross-linking agent stock solution;

[0032] 2) Mixing of cells and growth factors: add a single cell or multiple cells to the biological material stock solution according to the set concentration, mix well and put it int...

Embodiment 2

[0037]This example is basically the same as Example 1, and the special features are as follows: Step 1) Stock solution preparation: the biological material is mainly composed of sodium alginate, and deionized water is used as a solvent to prepare a concentration of 3-5% (w / v) biological material stock solution; in addition, hydroxyapatite, chitosan or gelatin materials can also be added, and the mass ratio of the added amount to sodium alginate is (0.5~1.5):1 to improve the life structure (6 ) mechanical and biological properties; the cross-linking agent is calcium chloride, and the cross-linking and solidification of the biological material stock solution is realized through the rapid diffusion of calcium ions and the chelation reaction with sodium alginate; the calcium chloride is prepared with deionized water as a solvent Make a cross-linking agent stock solution with a concentration of 3~4% (w / v). The combination of the biological material stock solution and the crosslink...

Embodiment 3

[0042] Example 3: Preparation of Bone Tissue Structure

[0043] In this case, cell manipulation is involved, which needs to be carried out in a sterile environment. Therefore, the experimental equipment and materials should be sterilized before the experiment. The experiment is carried out in an ultra-clean bench. The specific steps are as follows:

[0044] (1) Stock solution preparation: Weigh 2 g each of sodium alginate (Alg) and hydroxyapatite (HA) with an electronic balance, mix the two materials evenly by ball milling, dissolve in 50 ml of deionized water, stir and oscillate until completely dissolved, vacuum After defoaming, let it stand for later use; weigh 3g of calcium chloride, dissolve it in 100ml of deionized water, stir and oscillate until completely dissolved.

[0045] (2) Mixing of cells and growth factors: drop osteoblasts and endothelial cells into the mixed solution of Alg and HA, so that the concentration of both cells is 2×10 6 cells / ml, then fill the cel...

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Abstract

The invention discloses a one-stop preparation method of a vascularized life structure body. The method is capable of achieving integrated processing of biological materials, cells, growth factors and the like, and forming a life structure body with a vascularization function and a function of regional assembly of cells in a direct 3D printing manner. Annular biological material stoste is subjected to cross-linking solidification from inside to outside to form a similar vascular hollow fiber by virtue of coaxial shower nozzles; a macroscopic geometry structure of the life structure body is built by the similar vascular hollow fiber; concentration gradient along the radial direction of a fiber wall is formed by diffusion of growth factors; and one cell in a multi-cellular system is selectively transferred, thus achieving separation and assembly of microscale cells. The one-stop preparation method disclosed by the invention is capable of promoting progress of bio-manufacturing towards integrated and functional directions, and improving the implanting survival rate of the life structure body.

Description

technical field [0001] The invention relates to a preparation method of a novel vascularized life structure, belonging to the field of preparation of tissue engineering scaffolds. Background technique [0002] Life structures are composed of tissue engineering scaffolds, cells, growth factors, etc. Key technologies include structure construction, function realization and cell assembly. [0003] In recent years, biomanufacturing with 3D printing technology as the core provides a technical basis for the rapid and direct construction of the macroscopic geometry of living structures. However, there are still many problems that cannot be solved in terms of functional construction. For example, the vascularization function is extremely important for the nutrient supply, gas exchange, metabolite excretion, and growth factor delivery of cells in tissues. At present, in the field of tissue engineering and clinical medicine, growth factors, cell co-culture, implantation in a rich b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/38A61L27/20A61L27/22A61L27/12
Inventor 胡庆夕李瑜刘媛媛陈海萍张亚男李帅梁刚
Owner 上海蓝衍生物科技有限公司
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