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Water-soluble fluorescent magnetosome and preparation method thereof

A magnetosome, water-soluble technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-optics, etc., can solve the problems of poor repeatability, harsh reaction conditions, and biological toxicity, and achieves simple preparation method, Easy to operate, high fluorescence intensity effect

Inactive Publication Date: 2015-03-04
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The above-mentioned methods all have the disadvantages of biological toxicity, harsh reaction conditions, troublesome operation, long experimental cycle, and poor repeatability, which are not suitable for large-scale promotion and greatly limit the application of fluorescent magnetosomes.

Method used

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  • Water-soluble fluorescent magnetosome and preparation method thereof
  • Water-soluble fluorescent magnetosome and preparation method thereof

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Embodiment 1

[0036] This embodiment provides a method for preparing water-soluble fluorescent magnetosomes, comprising the following steps:

[0037] (1) Extraction of magnetosomes: Magnetosomes were obtained by magnetic separation after ultrasonic crushing of magnetotactic bacteria AMB-1;

[0038] (2) Magnetosome surface modification: Under the condition of stirring, the magnetosomes obtained in step (1) and cysteine ​​were reacted at room temperature for 24 hours at the pH value of the reaction system at 12; wherein, the The mass ratio of magnetosomes to cysteine ​​is 1:10.

[0039] Specifically, the preparation method of the present embodiment is as follows:

[0040]Take one liter of culture medium of magnetotactic bacteria AMB-1, centrifuge to obtain magnetotactic bacteria AMB-1, dissolve the magnetotactic bacteria AMB-1 in water, use ultrasonic crushing method to obtain magnetosomes, freeze-dry to obtain dry Magnetosomes are retained. Weigh 2.42 mg of magnetosomes and add them to 10...

Embodiment 2

[0042] This embodiment provides a method for preparing water-soluble fluorescent magnetosomes, comprising the following steps:

[0043] (1) Extraction of magnetosomes: Magnetosomes were obtained by magnetic separation after ultrasonic crushing of magnetotactic bacteria AMB-1;

[0044] (2) Magnetosome surface modification: Under the condition of stirring, the magnetosomes obtained in step (1) and cysteine ​​were reacted at room temperature for 24 hours at the pH value of the reaction system at 12; wherein, the The mass ratio of magnetosomes to cysteine ​​is 1:20.

[0045] Specifically, the preparation method of the present embodiment is as follows:

[0046] Take one liter of culture medium of magnetotactic bacteria AMB-1, centrifuge to obtain magnetotactic bacteria AMB-1, dissolve magnetotactic bacteria AMB-1 in water, use ultrasonic crushing method to obtain magnetosomes, freeze-dry to obtain dry magnetosomes The body is retained. Weigh 2.42 mg of magnetosomes and add them ...

Embodiment 3

[0048] This embodiment provides a method for preparing water-soluble fluorescent magnetosomes, comprising the following steps:

[0049] (1) Extraction of magnetosomes: Magnetosomes were obtained by magnetic separation after ultrasonic crushing of magnetotactic bacteria AMB-1;

[0050] (2) Magnetosome surface modification: Under the condition of stirring, the magnetosomes obtained in step (1) and cysteine ​​were reacted at room temperature for 24 hours at the pH value of the reaction system at 12; wherein, the The mass ratio of magnetosomes to cysteine ​​is 1:30.

[0051] Specifically, the preparation method of the present embodiment is as follows:

[0052] Take one liter of culture medium of magnetotactic bacteria AMB-1, centrifuge to obtain magnetotactic bacteria AMB-1, dissolve magnetotactic bacteria AMB-1 in water, use ultrasonic crushing method to obtain magnetosomes, freeze-dry to obtain dry magnetosomes The body is retained. Weigh 2.42 mg of magnetosomes and add them ...

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Abstract

The invention provides a water-soluble fluorescent magnetosome and its preparation method. The water-soluble fluorescent magnetosome contains the following ingredients: magnetosome and cysteine. In addition, cysteine modifies the surface of magnetosome. According to the invention, a cysteine-modified fluorescent magnetosome is prepared by a biomineralization-surface modification method. The fluorescent magnetosome is water-soluble and has good fluorescent stability and high fluorescence intensity. Meanwhile, the preparation method is simple and easy to operate, and no toxic substance participates in the preparation. According to the invention, application of the fluorescent magnetosome in magnetic targeting and fluorescence trace is greatly enhanced.

Description

technical field [0001] The invention relates to the field of preparation of fluorescent magnetic materials, in particular to a water-soluble fluorescent magnetosome and a preparation method thereof. Background technique [0002] Magnetotactic bacteria are a special kind of bacteria that can move along the geomagnetic field or an external magnetic field. In 1975, American biologist Richard Blakemore discovered that a class of microorganisms always moved in one direction when studying spirochetes in seabed mud. When the external magnetic field was changed, its direction of movement would also change. There is a tendency for the magnetic field to Sexual behavior, so he named this microorganism magnetotactic bacteria. The morphology and system of magnetotactic bacteria are diverse, including bacilli, vibrios, spirochetes, cocci, and multicellular bacteria. See figure 1 , figure 1 Electron micrographs of different forms of magnetotactic bacteria and magnetosome chains, where ...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/60G01N21/64B82Y20/00B82Y30/00
Inventor 安学勤张博卢鑫
Owner EAST CHINA UNIV OF SCI & TECH
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