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Method for determining residual quantity of conventional bactericides in tobacco

A technology of fungicides and residues, which is applied in the field of determination of commonly used fungicides residues in tobacco, can solve the problems of tediousness and complicated detection process, and achieve the effects of improving separation ability, reducing matrix effect and improving analysis sensitivity

Active Publication Date: 2015-03-04
SICHUAN BRANCH OF CHINA TOBACCO
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Problems solved by technology

In 2011, the tobacco industry formulated a series of multi-pesticide detection standards, and these 6 common pesticides were listed in two detection standards and three different detection methods, which made the detection process more complicated and cumbersome

Method used

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  • Method for determining residual quantity of conventional bactericides in tobacco
  • Method for determining residual quantity of conventional bactericides in tobacco
  • Method for determining residual quantity of conventional bactericides in tobacco

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experiment example 1

[0044] 1. Instruments and reagents: Waters Xevo TQ ultra-high performance liquid chromatography-tandem mass spectrometer (Waters, USA), equipped with electrospray ionization source (ESI); VtexMixer 230VeU oscillator (Labnet, USA); Sigma 3K15 centrifuge (Sigma, Germany) .

[0045] Formic acid is HPLC grade (concentration is 49-51%, Sigma Company, Germany); acetonitrile and methanol are chromatographically pure (Thermo-Fisher Company, USA); propamocarb, carbendazim, oxaxyl, metalaxyl, and rice blast Standards of thiophanate-methyl and thiophanate-methyl were obtained from Labor Dr. Ehrenstorfer-Schafers (chemical purity: 98.5%, Augsburg, Germany), internal standard carbendazim-d 4 From TRC Canada (chemical purity: 98%, isotopic purity: 98.9% and 99.2%, Toronto, Canada), thiophanate-d 6 Purchased from CDN Isotopes Inc. (chemical purity: 98%, isotopic purity: 99.9%, Quebec Canada). Reagent packs and N-propylethylenediamine (PSA) were purchased from Agilent (USA), and water was ...

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Abstract

The invention discloses a method for determining residual quantity of conventional bactericides in tobacco. The conventional bactericides are propamocarb, carbendazim, oxadixyl, metalaxyl, isoprothiolane and thiophanate-methyl. The method comprises the following steps: extracting a target object in tobacco, purifying by N-propyl ethylenediamine, preparing a standard storage solution and a standard working solution, and determining the residual quantity by virtue of ultrahigh-performance liquid phase chromatography-tandem mass spectrometry. Aiming at six conventional bactericides in the tobacco, UPLC-MS / MS is established for rapidly analyzing the residual quantity of the six bactericides in the tobacco rapidly by utilizing the ultrahigh-performance liquid phase chromatography-tandem mass spectrometer and an isotope internal standard quantitative method. Compared with the standard method, the detection indexes of two standard methods are merged; by adding propamocarb, the sensitivity of the method is improved, the analysis time is shortened, and the detection cost is reduced.

Description

technical field [0001] The invention belongs to the field of detection of pesticide residues, relates to the detection technology of pesticide residues in tobacco, in particular to a method for measuring the residues of fungicides commonly used in tobacco, specifically detecting propamocarb, carbendazim, oxaxyl, and metalaxyl in tobacco , blastazim and thiophanate-methyl residues. Background technique [0002] Propamocarb, carbendazim, oxaxyl, metalaxyl, rice blastexyl and thiophanate-methyl are all fungicides commonly used in tobacco production, which can prevent and control various fungal diseases of tobacco leaves, stems and roots. It has the characteristics of high efficiency, low toxicity, and both protective and therapeutic effects. Except for carbendazim, the other five indicators are included in the "China Tobacco Leaf Company's 2013 List of Pesticides Recommended for Tobacco Use" and are applied in various tobacco areas. Due to the widespread use of fungicides, la...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 陶晓秋熊巍庞夙韶济民黄玫
Owner SICHUAN BRANCH OF CHINA TOBACCO
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