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Method for extracting and purifying flavones from plants containing flavones

A technology of flavonoids and plants, applied in the field of extraction of bioflavonoids, can solve the problems of difficult application, good separation effect, clean process, etc., and achieve the effect of reducing extraction cost, increasing extraction rate, and high purification efficiency

Active Publication Date: 2015-03-11
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Yu Zhang et al. (Yu Zhang, Jingjing Jiao, Chengmei Liu, Xiaoqin Wu, Ying Zhang. Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin column chromatography and preparative high-performance liquid chromatography. Food Chemistry 107 2008) 1326-1336) proposed a method for purifying bamboo leaf flavonoids using macroporous resin and HPLC. Although this method is effective, it is difficult to be applied in industry
[0005] In short, there have been many reports on the extraction and purification of flavonoids, but a method with low energy consumption, short time, clean process, simple process and good separation effect has not been found so far. Therefore, a new and efficient flavonoid extraction and purification technology has been developed. very necessary

Method used

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  • Method for extracting and purifying flavones from plants containing flavones

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Collect fresh bamboo leaves, wash and dry them, and crush them to 60 mesh for later use. Put the bamboo leaf powder into the Erlenmeyer flask, add 60% ethanol aqueous solution, the ratio is 1:20 (g / g), the mass fraction is 0.9% surfactant-sodium dodecylsulfonate, and soak for 0.5h. Put the Erlenmeyer flask into a microwave oven, set the microwave power to 450W and the extraction time to 1min. After the microwave extraction was completed, the Erlenmeyer flask was taken out and cooled to room temperature, and then filtered under reduced pressure to obtain the filtrate for use. The ethanol and water in the filtrate are evaporated and recovered to obtain a concentrated solution 1, the concentration of flavonoids in the concentrated solution 1 is about 1 mg / ml, and the concentrated solution 1 is freeze-dried to obtain crude flavonoid particles. The resulting concentrated solution 1 is absorbed by the macroporous resin AB-8, analyzed with 2 times the bed volume of 95% ethano...

Embodiment 2

[0024]Similar to case 1, but without adding surfactant. Fresh bamboo leaves were collected, washed and dried, and crushed to 45 mesh for use. Put the bamboo leaf powder into a conical flask, add 60% ethanol aqueous solution at a ratio of 1:50 (g / g), and soak for 0.5h. Put the Erlenmeyer flask into a microwave oven, set the microwave power to 225W and the extraction time to 2min. After the microwave extraction was completed, the Erlenmeyer flask was taken out and cooled to room temperature, and then filtered under reduced pressure to obtain the filtrate for use. The ethanol and water in the filtrate are evaporated and recovered to obtain a concentrated solution 1, the concentration of flavonoids in the concentrated solution 1 is about 1 mg / ml, and the concentrated solution 1 is freeze-dried to obtain crude flavonoid particles. The resulting concentrated solution 1 is absorbed by the macroporous resin AB-8, analyzed with 2.5 times the bed volume of 95% ethanol solution, and th...

Embodiment 3

[0026] Fresh bamboo leaves were collected, washed and dried, and crushed to 45 mesh for use. Put the bamboo leaf powder into a conical flask, add 60% ethanol aqueous solution, the ratio is 1:20 (g / g), and the mass fraction is 0.5% surfactant-sodium dodecylbenzenesulfonate, soak for 0.5h . Put the Erlenmeyer flask into a microwave oven, set the microwave power to 150W and the extraction time to 1min. After the microwave extraction was completed, the Erlenmeyer flask was taken out and cooled to room temperature, and then filtered under reduced pressure to obtain the filtrate for use. The ethanol and water in the filtrate are evaporated and recovered to obtain a concentrated solution 1, the concentration of flavonoids in the concentrated solution 1 is about 1 mg / ml, and the concentrated solution 1 is freeze-dried to obtain crude flavonoid particles. The resulting concentrated solution 1 is absorbed by the macroporous resin AB-8, analyzed with 2 times the bed volume of 95% ethan...

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Abstract

The invention discloses a method for extracting and purifying flavones from plants containing flavones, and relates to extraction of bioflavonoids. The method is used for effectively extracting and purifying flavones from plants containing flavones, and has the advantages of simple extraction and purification process, low energy consumption, short time, high recovery rate of the flavones, clean and pollution-free production process, and high product purity. The method comprises the following steps: 1, crushing the plants containing flavones, adding the obtained plant powder into an ethanol-water solution containing a surfactant, and immersing to obtain a mixture; 2, carrying out microwave treatment on the mixture in a microwave device, taking out the microwave treated mixture, cooling to room temperature, and carrying out reduced pressure pumping filtration to obtain a filtrate containing flavones; 3, carrying out evaporative concentration on the filtrate containing flavones, carrying out macro-porous resin adsorption, and eluting to obtain an eluate; and 4, carrying out evaporative concentration on the eluate to obtain a concentrate liquid, and freeze-drying the concentrate liquid to obtain the flavones.

Description

technical field [0001] The invention relates to the extraction of bioflavonoids, in particular to a method for extracting and purifying flavones from plants containing flavones. technical background [0002] Flavonoids have many effects. It is a strong antioxidant that can effectively remove oxygen free radicals in the body. For example, anthocyanins can inhibit the full-stage overflow of oily peroxides. This ability to prevent oxidation is a vitamin More than 10 times that of E, this antioxidant effect can prevent the degeneration and aging of cells, and also prevent the occurrence of cancer. [0003] Flavonoids can improve blood circulation and lower cholesterol. The flavonoids in Tianguo also contain a PAF anticoagulant factor. These effects greatly reduce the incidence of cardiovascular and cerebrovascular diseases, and can also improve the symptoms of cardiovascular and cerebrovascular diseases. The sky fruit from the South Pacific island country is rich in 33 flavonoi...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K36/185A61K36/287A61K36/62A61K36/738A61K36/752A61K36/70A61K36/899
CPCA61K36/16A61K36/185A61K36/287A61K36/62A61K36/70A61K36/738A61K36/752A61K36/899A61K2236/333A61K2236/51
Inventor 刘运权胡桂萍张彬彬
Owner XIAMEN UNIV
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