Anti-aging short peptide and preparation method thereof

An anti-aging, short peptide technology, applied in the field of genetic engineering, can solve the problems of limited widespread use, high cost, limited chemical synthesis, etc., and achieve the effects of easy promotion, low production cost, and simple production process

Active Publication Date: 2017-06-30
SHANXI JINBO BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current preparation methods of palmitic acid pentapeptide-3 are still limited to chemical synthesis, and its high cost limits the widespread use of such products

Method used

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  • Anti-aging short peptide and preparation method thereof
  • Anti-aging short peptide and preparation method thereof
  • Anti-aging short peptide and preparation method thereof

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Experimental program
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Embodiment Construction

[0038] Specific embodiments of the present invention will be described in detail below.

[0039] A preparation method of anti-aging short peptide, comprising the steps of:

[0040] (1) Construction of Escherichia coli genetically engineered bacteria

[0041]1.1. In order to obtain the anti-aging short peptide rG13 after enzyme digestion, the amino acid sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro-Lys-Thr-Thr-Lys-Ser is specially designed as the basic repeating unit L13, and the protein is constructed Sequence pL13-n.

[0042] The number of repeats of this unit is directly proportional to the yield of the final short peptide. Usually 1-100 recombination units can be used for construction, and the target protein sequence (LEVLFQGPKTTKS) constructed in this way n It is abbreviated as pL13-n, where n is any value from 1 to 100; experiments have shown that when n=9, the yield of the anti-aging short peptide rG13 is higher.

[0043] In this example, 9 repeating units are taken as an...

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Abstract

The invention relates to a preparation method of recombinant protein in the field of genetic engineering, in particular to a novel anti-aging short peptide and a preparation method. The invention discloses an anti-aging short peptide named G13, which is composed of 13 amino acids; and discloses a process of using Escherichia coli fermentation, which can realize industrialized large-scale preparation of recombinant G13 (referred to as rG13). The present invention detects and confirms that rG13 has higher biological activity than similar products, can stimulate cells to secrete a large amount of collagen, and has anti-aging ability. The anti-aging short peptide of the invention has obvious biological activity, simple production process and low cost, can make the anti-aging skin nutrition product approach the daily life of the common people, and has broad market application prospects.

Description

technical field [0001] The invention relates to a design and preparation method of a recombinant protein in the field of genetic engineering, in particular to a novel anti-aging short peptide and a preparation method thereof. Background technique [0002] The skin of humans and higher animals consists of epidermis, dermis, and subcutaneous tissue. The epidermis has no blood vessels, and the energy and nutrition of the cells must be provided by the microcirculation of the dermis. The dermis is the lifeblood of the skin. It is a layer of dense, tough and elastic tissue, mainly composed of fibrous components (collagen fibers, elastic fibers and reticular fibers), glycoproteins, glucosamine and hyaluronic acid. Hyaluronic acid is a transparent gel-like substance with a water absorption capacity of 500-1000 times, and is recognized as the best moisturizing product. Young skin, with high hyaluronic acid content, makes the skin smooth, elastic, supple, plump; when the skin ages, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12N15/70A61P17/16
Inventor 杨霞
Owner SHANXI JINBO BIO PHARMA CO LTD
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