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Extraction technology of cordyceps militaris polysaccharide and polypeptide

A technology of Cordyceps militaris polysaccharides and extraction process, which is applied in fermentation and other directions, can solve problems affecting the separation and purification of Cordyceps militaris polypeptides, and achieve the effects of simple and easy-to-operate extraction methods, mild extraction conditions, and high biological activity

Inactive Publication Date: 2015-03-11
SUZHOU COSMETIC MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the enzymolysis solution obtained after enzymatic hydrolysis of the residue of Cordyceps militaris HWM was brown and darker, which affected the subsequent separation and purification of Cordyceps militaris polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A process for extracting polysaccharides and polypeptides from Cordyceps militaris, comprising the steps of:

[0019] 1) Dried Cordyceps militaris, crushed to 100 mesh, added distilled water, stirred evenly, and then ultrasonicated for 1 min; the mass-volume ratio of Cordyceps militaris to distilled water was 1:18, and the unit was g / mL.

[0020] 2) Heat the aqueous solution of Cordyceps militaris after ultrasonic treatment in step 1) to 90°C, stir and react for 20 minutes, then collect the filter cake and filtrate by filtration, add ethanol to the filtrate, let it stand, and precipitate the polysaccharide of Cordyceps militaris, the extraction rate is 65%;

[0021] 3) Add the filter cake collected in step 2) into distilled water, stir, adjust the pH of the solution to 8 with sodium hydroxide, add alkaline protease to hydrolyze for 8 hours, the amount of alkaline protease added is 4000U per gram of filter cake, boil for 3 minutes, and stop Enzymolysis, filtration, and H...

Embodiment 2

[0023] A process for extracting polysaccharides and polypeptides from Cordyceps militaris, comprising the steps of:

[0024] 1) Dried Cordyceps militaris, crushed to 80 mesh, added distilled water, stirred evenly, and then ultrasonicated for 1-3 minutes; the mass-volume ratio of Cordyceps militaris to distilled water was 1:10, and the unit was g / mL.

[0025] 2) Heat the aqueous solution of Cordyceps militaris after ultrasonic treatment in step 1) to 70°C, stir and react for 30 minutes, then collect the filter cake and filtrate by filtration, add ethanol to the filtrate, let it stand, and precipitate the polysaccharide of Cordyceps militaris, the extraction rate is 63%;

[0026] 3) Add the filter cake collected in step 2) into distilled water, stir, adjust the pH of the solution to 10 with sodium hydroxide, add alkaline protease to hydrolyze for 8 hours, the amount of alkaline protease added is 5000U per gram of filter cake, boil for 3 minutes, and stop Enzymolysis reaction, fi...

Embodiment 3

[0028] A process for extracting polysaccharides and polypeptides from Cordyceps militaris, comprising the steps of:

[0029] 1) Dried Cordyceps militaris, crushed to 80 mesh, added distilled water, stirred evenly, and then ultrasonicated for 2 minutes; the mass-volume ratio of Cordyceps militaris to distilled water was 1:18, and the unit was g / mL.

[0030] 2) Heat the aqueous solution of Cordyceps militaris after ultrasonic treatment in step 1) to 80°C, stir and react for 30 minutes, then collect the filter cake and filtrate by filtration, add ethanol to the filtrate, let it stand, and precipitate the polysaccharide of Cordyceps militaris, the extraction rate is 66%;

[0031] 3) Add the filter cake collected in step 2) into distilled water, stir, adjust the pH of the solution to 9 with sodium hydroxide, add alkaline protease to hydrolyze for 10 hours, the amount of alkaline protease added is 5000U per gram of filter cake, boil for 2 minutes, and stop Enzymolysis, filtration, a...

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Abstract

The invention discloses an extraction technology of cordyceps militaris polysaccharide and polypeptide. The extraction technology comprises the following steps: drying cordyceps militaris, grinding cordyceps militaris to a size of 80 to 120 meshes, adding distilled water, evenly stirring, carrying out a supersonic treatment for 1 to 3 minutes; heating to a temperature of 60 to 90 DEG C, carrying out reactions for 20 to 60 minutes under stirring, filtering, collecting the filter cakes and the filtrate, adding ethanol into the filtrate, allowing the filtrate to stand still to precipitate cordyceps militaris polysaccharide; adding the filter cakes into distilled water, stirring, adjusting the pH value of the solution to 8-10 by sodium hydroxide, adding alkaline protease to carry out hydrolysis for 8 to 12 hours, boiling the solution, stopping the enzyme hydrolysis reactions, filtering, and subjecting the filtrate to HPLC purification so as to obtain the cordyceps militaris polypeptide. The provided extraction technology can extract cordyceps militaris polysaccharide and polypeptide at the same time, fully utilizes the cordyceps militaris resources, and moreover has the advantages of mild extraction conditions, high product bioactivity, and simple and easy extraction method.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a process for extracting polysaccharides and polypeptides from Cordyceps militaris. Background technique [0002] The pace of life in modern society is fast, and most people are in a sub-healthy state, and diseases such as inflammation, cancer, and tumors occur from time to time. Cordyceps militaris is a fungus that can be used as both food and medicine. Its active ingredients such as Cordyceps polypeptide and polysaccharides have significant effects in medical treatment, health care, diet and other aspects. Therefore, the development and research of Cordyceps militaris series products should be paid attention to. [0003] Relevant studies have shown that Cordyceps militaris is rich in three types of nutrients, protein, lipid, and sugar, as well as a variety of vitamins and trace elements. It is rich in cordycepic acid (mannitol), cordycepin (3'-deoxyaden...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P21/06
Inventor 金仲恩全春兰张帆
Owner SUZHOU COSMETIC MATERIALS
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