Extraction technology of cordyceps militaris polysaccharide and polypeptide

Abstract

The invention discloses an extraction technology of cordyceps militaris polysaccharide and polypeptide. The extraction technology comprises the following steps: drying cordyceps militaris, grinding cordyceps militaris to a size of 80 to 120 meshes, adding distilled water, evenly stirring, carrying out a supersonic treatment for 1 to 3 minutes; heating to a temperature of 60 to 90 DEG C, carrying out reactions for 20 to 60 minutes under stirring, filtering, collecting the filter cakes and the filtrate, adding ethanol into the filtrate, allowing the filtrate to stand still to precipitate cordyceps militaris polysaccharide; adding the filter cakes into distilled water, stirring, adjusting the pH value of the solution to 8-10 by sodium hydroxide, adding alkaline protease to carry out hydrolysis for 8 to 12 hours, boiling the solution, stopping the enzyme hydrolysis reactions, filtering, and subjecting the filtrate to HPLC purification so as to obtain the cordyceps militaris polypeptide. The provided extraction technology can extract cordyceps militaris polysaccharide and polypeptide at the same time, fully utilizes the cordyceps militaris resources, and moreover has the advantages of mild extraction conditions, high product bioactivity, and simple and easy extraction method.

Description

technical field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a process for extracting polysaccharides and polypeptides from Cordyceps militaris. Background technique

[0002] The pace of life in modern society is fast, and most people are in a sub-healthy state, and diseases such as inflammation, cancer, and tumors occur from time to time. Cordyceps militaris is a fungus that can be used as both food and medicine. Its active ingredients such as Cordyceps polypeptide and polysaccharides have significant effects in medical treatment, health care, diet and other aspects. Therefore, the development and research of Cordyceps militaris series products should be paid attention to.

[0003] Relevant studies have shown that Cordyceps militaris is rich in three types of nutrients, protein, lipid, and sugar, as well as a variety of vitamins and trace elements. It is rich in cordycepic acid (mannitol), cordycepin (3'-deoxyaden...