Purification and renovation method for species of spirulina

A technology for purifying rejuvenation and spirulina, applied in the direction of bacteria, etc., can solve the problems of reduced harvest, affecting the yield of spirulina, affecting the growth of spirulina, and achieving the effect of solving the problem of degradation

Inactive Publication Date: 2015-03-11
GUANGDONG MEIYAN HYDROPOWER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of organic compound nutrients are used in the production process of artificial breeding, which causes the degradation of spirulina species, affects the growth of spirulina, and reduces the harvest, which in turn affects the output of spirulina and increases the cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Proportion of nutrient solution: ferrous sulfate 0.01kg / t, calcium chloride 0.01kg / t, potassium chloride 0.5kg / t, sodium nitrate 0.5kg / t, sodium chloride 0.5kg / t, sodium sulfate 0.3 kg / t, magnesium sulfate 0.15kg / t, urea 0.05kg / t, potassium dihydrogen phosphate 0.15kg / t, baking soda 3kg / t.

[0017] (2) Take 50 grams of spirulina mud to break the cells, and add 400 grams of nutrient solution after 3 minutes.

[0018] (3) Irradiate with ultraviolet light for two consecutive days, start to irradiate for 1 hour at 8:00 am every day, and the temperature of the breeding water body is 25-35°C.

[0019] (4) On the third day, add 250 grams of supplementary culture medium and cultivate for 10 days. The supplementary culture medium is prepared by adding 55 grams of boric acid, 35 grams of manganese chloride, 4 grams of zinc sulfate, 12 grams of copper sulfate and 1.5 grams of ammonium molybdate per ton of water.

[0020] In this embodiment, in the process of purifying and re...

Embodiment 2

[0022] (1) Proportion of nutrient solution: ferrous sulfate 0.012kg / t, calcium chloride 0.02kg / t, potassium chloride 0.7kg / t, sodium nitrate 0.75kg / t, sodium chloride 0.8kg / t, sodium sulfate 0.5 kg / t, magnesium sulfate 0.25kg / t, urea 0.08kg / t, potassium dihydrogen phosphate 0.25kg / t, baking soda 6kg / t.

[0023] (2) Take 50 grams of spirulina mud to break the cells, and add 500 grams of nutrient solution after 5 minutes.

[0024] (3) Irradiate with ultraviolet light for three consecutive days, start to irradiate for 2 hours at 8:00 am every day, and the temperature of the aquaculture water body is 25-35°C.

[0025] (4) On the fourth day, add 300 grams of auxiliary culture medium and cultivate for 8 days. Among them, the auxiliary culture medium is diluted by adding 57g of boric acid, 37g of manganese chloride, 4.5g of zinc sulfate, 1.6g of copper sulfate and 1.7g of ammonium molybdate per ton of water.

[0026] In this embodiment, in the process of purifying and rejuvenating ...

Embodiment 3

[0028] (1) Proportion of nutrient solution: ferrous sulfate 0.015kg / t, calcium chloride 0.05kg / t, potassium chloride 1kg / t, sodium nitrate 1kg / t, sodium chloride 1kg / t, sodium sulfate 0.8kg / t , magnesium sulfate 0.5kg / t, urea 0.1kg / t, potassium dihydrogen phosphate 0.5kg / t, baking soda 8kg / t.

[0029] (2) Take 50 grams of spirulina mud for cell disruption, and add 400 grams of nutrient solution after 10 minutes.

[0030] (3) Irradiate with ultraviolet light for five consecutive days, starting at 9:00 am every day for 2 hours, and the temperature of the cultured water body is 25-35°C.

[0031] (4) On the sixth day, add 500 grams of auxiliary culture medium and cultivate for 5 days. The auxiliary culture medium is prepared by adding 60g of boric acid, 40g of manganese chloride, 5g of zinc sulfate, 2g of copper sulfate and 2g of ammonium molybdate per ton of water.

[0032] In this embodiment, in the process of purifying and rejuvenating algal species in spirulina cultivation, ...

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PUM

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Abstract

The invention discloses a purification and renovation method for species of spirulina, which belongs to the technical field of species culture of spirulina. The method comprises the following steps: 1)preparing a nutrient solution; 2)taking spirulina slurry for cell disruption, adding the nutrient solution after 3-10 minutes, wherein the weight ratio of spirulina slurry to the nutrient solution is 1: 8-12; 3) continuously using ultraviolet light for irradiation for 2-5 days, wherein irradiation time per day is 1-2 hours, and the temperature of culture water is 25-35 DEG C; and 4)adding an auxiliary nutrient solution for culture for 5-10 days after the next day of completion of the ultraviolet light irradiation. The method has the advantages of simple operation and low cost, can increase the spirulina output, and can be used for culturing species of spirulina.

Description

technical field [0001] The invention relates to a method for cultivating spirulina species, more specifically, to a method for purifying and rejuvenating spirulina species. Background technique [0002] Spirulina belongs to the phylum Cyanophyta, a kind of photoautotrophic organism of Oscillatoriaceae. There is no real nucleus in the cells, so it is also called cyanobacteria. Studies have shown that spirulina has high protein content and reasonable amino acid composition, among which the content of 8 essential amino acids is close to the standard recommended by FAO. It is the most excellent, most comprehensive and balanced pure natural protein food source discovered by scientists so far. . Because the cell wall of spirulina is composed of polysaccharides, it is easy to digest and absorb, and the bioavailability reaches 68%. At the same time, spirulina is also rich in vitamins and various trace elements, which have the effects of enhancing immunity, anti-virus, lowering bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 朱宝荣田健黄平娜汪汝俊叶选荣林焕癸
Owner GUANGDONG MEIYAN HYDROPOWER CO LTD
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