Method for knockout of human papillomavirus E6E7 gene by zinc finger nucleases

A technology of human papillomavirus and zinc finger nuclease, which is applied in the fields of genetic engineering, plant genetic improvement, botany equipment and methods, etc., and can solve problems such as limitation of drug delivery methods and poor stability
CN104404076AActive Publication Date: 2015-03-11WUHAN KDWS BIOLOGICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN KDWS BIOLOGICAL TECH CO LTD
Publication Date
2015-03-11

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Abstract

Relating to the field of gene therapy, the invention provides a method for knockout of a high-risk human papillomavirus (HPV) E6E7 gene by zinc finger nucleases (ZFNs). According to a high-risk type HPV E6E7 gene sequence, the method designs ZFNs expression vectors of a targeted specific site to targetedly cut the HPVE6E7 gene sequence in cells, thereby knocking out a target gene. In corresponding subtype HPV infected cells, the ZFNs can significantly induce cell apoptosis and proliferation inhibition, and has no effect on other subtype HPV positive cells and HPV negative cells. Transfection of the ZFNs expression vectors in a subcutaneous tumor model of HPV positive cervical cancer cells can obviously inhibit the growth rate of subcutaneous tumors and alleviate tumors. The method for targeted knockout of the high-risk HPV E6E7 gene by ZFNs can efficiently destroy virus oncogenes at the DNA level so as to make HPV infected cells undergo apoptosis and proliferation inhibition, and has enormous treatment value to HPV infection related diseases, especially cervical intraepithelial neoplasias and other precancerous lesions.
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Description

technical field

[0001] The present invention relates to the field of gene therapy, and the disclosure includes constructing a targeted zinc finger nuclease for the high-risk human papillomavirus E6E7 oncogene sequence, and using the zinc finger nuclease to knock out the high-risk human papillomavirus E6E7 oncogene Methods. Background technique

[0002] Cervical cancer is one of the most common gynecological malignancies in women worldwide after breast cancer, and its incidence rate ranks second among female malignancies. Its incidence rate ranks first in some developing countries. According to the International Cancer Research Center, there are approximately 3,710,200 new cases of cervical cancer each year, accounting for 9.8% of all tumors and 15% of new cases in women, of which 78% occur in developing countries. my country has a vast territory and a large population. According to the large-scale national population death survey organized by the National Cancer Prevention...

Claims

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