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Drug testing enzyme linked immunosorbent assay kit and detection method thereof

An immune reagent and enzyme detection technology, which is applied in the field of contraband detection and analysis, can solve the problems of poor accuracy and low sensitivity of the colloidal gold method, and achieve the effects of reducing errors, simplifying the pretreatment process, and reducing operation steps

Inactive Publication Date: 2015-03-18
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently commonly used colloidal gold method is affected by the biological agents used, and the probability of false positives is high. Moreover, due to the low sensitivity of the colloidal gold method, the accuracy of color judgment is poor due to human factors.

Method used

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  • Drug testing enzyme linked immunosorbent assay kit and detection method thereof
  • Drug testing enzyme linked immunosorbent assay kit and detection method thereof
  • Drug testing enzyme linked immunosorbent assay kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 kit components

[0026] (1) Antigen synthesis

[0027] A. Synthesis of Morphine Artificial Antigen

[0028] Weigh 500 mg of morphine raw material, add 50 mL of benzene and 1 g of succinic anhydride, and heat to reflux for 8 h. Install the evaporating device and evaporate the benzene. The precipitate was washed with anhydrous ether and anhydrous ethanol respectively, and the residue was vacuum-dried to obtain powdery substance 6-succinic acid-morphine. Weigh 20 mg of the above powdered substance and dissolve it in 10 ml of PBS buffer (phosphate buffer), add 30 ul of triethylamine, then add 30 ul of isobutyl chloroformate, and react for half an hour. Weigh 20 mg of BSA and dissolve it in PBS buffer solution, add the above reaction solution, stir and react overnight, dialyze for 3 days, centrifuge in a high-speed refrigerated centrifuge, collect the supernatant to obtain the morphine antigen.

[0029] B. Synthesis of Methamphetamine Arti...

Embodiment 2

[0041] Example 2 Formation of Opioid Detection ELISA Kit

[0042] Construct the Opioid Detection ELISA Kit to include the following components:

[0043] (1) Morphine standard solution, the concentrations are 0ng / ml, 0.25ng / ml, 0.8ng / ml, 2.4ng / ml, 7ng / ml, 21ng / ml, 1ml / bottle, use the sample diluent described below dilution;

[0044] (2) ELISA plate coated with morphine-specific antibody, 1 piece, 96 wells;

[0045] (3) Morphinase marker, 1 bottle, 13ml;

[0046] (4) Chromogen: Tetramethylbenzidine phosphate buffer, 1 bottle, 13ml;

[0047] (5) Concentrated washing solution: containing 0.5% Tween, 1‰ sodium azide, 0.01mol / L PBS, 1 bottle, 50ml, 10 times the normal use concentration;

[0048] (6) Stop solution: 2mol / L sulfuric acid, 1 bottle, 13ml;

[0049] (7) Sample diluent: 0.01mol / L PBS, 1 bottle, 50ml.

Embodiment 3

[0050] Example 3 Formation of Amphetamine Detection ELISA Kit

[0051] Set up the amphetamine detection ELISA kit so that it contains the following components:

[0052] (1) Methamphetamine standard solution, the concentrations are 0ng / ml, 0.5ng / ml, 1.5ng / ml, 4.5ng / ml, 13.5ng / ml, 40.5ng / ml, 1ml / bottle, use the following The sample diluent is diluted;

[0053] (2) Enzyme plate coated with methamphetamine specific antibody, 1 piece, 96 wells;

[0054] (3) Methamphetamine enzyme marker, 1 bottle, 13ml;

[0055] (4) Chromogen: Tetramethylbenzidine phosphate buffer, 1 bottle, 13ml;

[0056] (5) Concentrated washing solution: containing 0.5% Tween, 1‰ sodium azide, 0.01mol / L PBS, 1 bottle, 50ml, 10 times the normal use concentration;

[0057] (6) Stop solution: 2mol / L sulfuric acid, 1 bottle, 13ml;

[0058] (7) Sample diluent: 0.01mol / L PBS, 1 bottle, 50ml.

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Abstract

The invention discloses an enzyme linked immunosorbent assay kit for detecting drugs in urine, saliva, blood and hairs in the technical field of the prohibited goods detection and analysis as well as a detection method of the drug testing enzyme linked immunosorbent assay kit. The kit comprises a drug standard substance solution, a drug specificity antibody, an elisa plate which is coated with the drug specificity antibody, a drug antigen enzyme marker, a color developing agent, concentrated washing liquor, a termination solution and a sample dilute solution. The enzyme linked immunosorbent assay kit has characteristics of high specificity, high sensitivity, high accuracy and the like and plays an important role in drug testing.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis of contraband, in particular to an enzyme-linked immunosorbent assay kit for detecting drugs in urine, saliva, blood and hair and a detection method thereof. Background technique [0002] Under the influence of the international drug epidemic, the drug situation in my country is very severe. As of the end of May 2013, the statistics show that there are currently 2.22 million drug users in China, and there are also many hidden drug users. Therefore, in order to prevent and punish drug-related crimes, protect citizens' physical and mental health, and maintain social order, it is necessary to establish accurate, reliable, and highly sensitive drug testing. [0003] Most opioid drugs are hallucinogenic, narcotic, and have analgesic effects, mainly including opium, marijuana, heroin, morphine, 6-monoacetylmorphine, and codeine. Amphetamine-type drugs refer to a class of synthetic compou...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/5308G01N33/535
Inventor 高颖魏建良
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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