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Preparation method of water-insoluble flexible silk fibroin membrane

A silk fibroin film and flexible technology, applied in the field of preparation of flexible silk fibroin film, can solve the problems of water solubility or poor mechanical properties of silk fibroin film, and achieve the effect of expanding application routes, simple process and good mechanical properties.

Active Publication Date: 2015-03-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the generally prepared silk fibroin film being soluble in water or having poor mechanical properties, and to provide a method for preparing a flexible silk fibroin film that is insoluble in water, with simple operation, mild reaction conditions and no introduction of other polymers. polymers to obtain a silk film that is insoluble in water and flexible in the dry state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In the present embodiment, the preparation method of flexible and insoluble water silk fibroin film comprises the following steps successively:

[0029] (1) Weigh 6g of shredded cocoon layer in 600ml of Na with a mass fraction of 0.5% 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0030] (2) Put 4.2g of silk fibroin fiber obtained in step (1) into 21ml of 9M LiBr solution, and dissolve it at 60°C for 4 hours. Dialyze in deionized water for 3 days, change the water every 2 hours, and finally concentrate the silk fibroin solution to a concentration of 8% (w / v).

[0031] (3) Weigh 0.2g of EDC powder and add it to the 50ml silk fibroin solution with a concentration of 8% obtained in step (2), so that the content of EDC is 5% of the silk fibroin protein in the silk fibroin solution (w / w) .

[0032] (4) Stir the mixture obtained in step (3) with ...

Embodiment 2

[0036] In the present embodiment, the preparation method of flexible and insoluble water silk fibroin film comprises the following steps successively:

[0037] (1) Weigh 5g of shredded cocoon layer in 500ml of Na with a mass fraction of 0.5%. 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0038] (2) Put 3.8g of silk fiber obtained in step (1) into 19ml of 9M LiBr solution, dissolve it at 60°C for 4 hours, and filter the obtained liquid into a dialysis bag (molecular weight 8000-14000) to remove Dialysis in deionized water was performed for 4 days, and the water was changed every 2 hours. Finally, the silk fibroin solution obtained was concentrated to a concentration of 7% (w / v).

[0039] (3) Weigh 0.245g of EDC powder and add it to the 50ml silk fibroin solution with a concentration of 7% obtained in step (2), so that the content of EDC is 7% of the...

Embodiment 3

[0044] In the present embodiment, the preparation method of flexible and insoluble water silk fibroin film comprises the following steps successively:

[0045] (1) Weigh 6g of shredded cocoon layer in 600ml of Na with a mass fraction of 0.5% 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0046] (2) Put 4.5g of silk fiber obtained in step (1) into 22.5ml of 9M LiBr solution, and dissolve it at 60°C for 4 hours. Dialyze in deionized water for 4 days, change the water every 2 hours, and finally concentrate the silk fibroin solution to a concentration of 8% (w / v).

[0047](3) Weigh 0.42g of EDC powder and add it to the 52.5ml silk fibroin solution with a concentration of 8% obtained in step (2), so that the content of EDC is 10% of the silk fibroin protein in the silk fibroin solution (w / w ).

[0048] (4) Stir the mixture obtained in step (3) with a m...

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Abstract

The invention discloses a preparation method of a water-insoluble flexible silk fibroin membrane. The preparation method comprises the following steps: degumming cocoon layers in a Na2CO3 boiling water solution for twice, then flushing by using deionized water, wringing, and drying the cocoon layers in a drying oven; putting silk fibroin fibers in an LiBr solution, performing vibration treatment, filtering, then feeding into a dialysis bag, and then concentrating a solution; adding EDC powder into a silk fibroin solution, stirring, completely dissolving, and uniformly mixing; and spreading a mixed solution on a polyethylene plate, naturally drying at normal temperature, or drying the mixed solution in the drying oven to prepare a silk fibroin membrane. The preparation method disclosed by the invention is simple to operate and mild in reaction condition; and meanwhile, other high-molecular polymers are not introduced, the silk fibroin membrane which is water-insoluble and is flexible in a dry state can be prepared, the silk fibroin membrane is expected to be applied to the fields of medicines and health, tissue engineering and the like, application routes of silk fibroin can be expanded, and a novel method for preparing high-molecular protein membranes with excellent performance can also be provided.

Description

technical field [0001] The invention relates to a method for processing silk fibroin, in particular to a method for preparing a water-insoluble flexible silk film. Background technique [0002] Silk fibroin is a natural polymer material. Because of its good biocompatibility, biodegradability, and mechanical properties, it has been more and more widely researched and applied in the fields of biomedicine, cosmetics, and food. Silk fibroin film has good dissolved oxygen permeability under wet conditions, and it is expected to be used to prepare tissue repair materials such as artificial skin. However, since regenerated silk fibroin is water-soluble and has poor flexibility in a dry state, the application range of silk fibroin membranes in the biomedical field is greatly limited. [0003] EDC (1-ethyl-(3-dimethylaminopropyl)diimide carbonate), with a molecular weight of 191.7, is a small molecular organic compound with no toxicity. In the present invention, EDC promotes the ch...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08K5/29C08J5/18
Inventor 朱良均潘彩霞许宗溥张海萍杨明英
Owner ZHEJIANG UNIV
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