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Method for purifying hydroxyl-containing pigments

A purification method and pigment technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of high cost, a large amount of organic solvents, and limited applications, and achieve low cost, simple process, and high purity of pigments Effect

Active Publication Date: 2016-06-01
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing methods for the separation and purification of monascus red pigment include column chromatography, thin plate chromatography, liquid chromatography, etc. Different substances, but homologous pigments, orange pigments, yellow pigments, amino acids and protein substances that have little difference in hydrophilicity with red pigments are difficult to separate
Moreover, purification by chromatography requires a large amount of organic solvents, chromatography materials and expensive instruments and equipment, which limits its application in industrialization due to high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) The corn starch emulsion with a mass percent concentration of 15% was gelatinized at 90° C. for 40 min, and then subjected to high-pressure treatment to obtain starch paste. The hydroxyl-containing monascus red pigment is dissolved in water so that the concentration of the aqueous solution of the hydroxyl-containing monascus red pigment is 0.01 mg / mL, and the amount of the hydroxyl-containing monascus red pigment is 5% of the dry cornstarch mass. The aqueous solution containing hydroxy monascus red pigment is mixed with starch paste, and then aged at 0°C for 24 hours to obtain colloidal retrograded starch containing monascus red pigment; the temperature of the high-pressure treatment is 110°C, and the time of high-pressure treatment is 20min; heat the obtained colloidal retrograded starch containing monascus red pigment in a water bath at a temperature of 85°C, and add 4% of the amount of starch with high-temperature amylase (with an activity of 200,000 U / ml) for enz...

Embodiment 2

[0030] (2) The sweet potato starch emulsion with a mass percent concentration of 25% was gelatinized at 95° C. for 40 min, and then subjected to high-pressure treatment to obtain starch paste. The hydroxyl-containing monascus red pigment is dissolved in water so that the concentration of the aqueous solution containing the hydroxyl-containing monascus red pigment is 0.5 mg / mL, and the amount of the hydroxyl-containing monascus red pigment is 10% of the dry sweet potato starch mass. The aqueous solution containing hydroxy monascus red pigment is mixed with starch paste, and then aged at 4°C for 48h to obtain gelatinous retrograded starch containing monascus red pigment; the temperature of the high-pressure treatment is 120°C, and the time of high-pressure treatment is 40min; the obtained colloidal retrograded starch containing monascus red pigment was placed in a water bath with a temperature of 95°C and heated, and 4% of the amount of starch was added with high-temperature amyl...

Embodiment 3

[0035] (1) The potato starch emulsion with a mass percent concentration of 20% was gelatinized at 90° C. for 40 min, and then subjected to high-pressure treatment to obtain starch paste. The hydroxyl-containing monascus red pigment is dissolved in water so that the concentration of the aqueous solution containing the hydroxyl-containing monascus red pigment is 0.1 mg / mL, and the amount of the hydroxyl-containing monascus red pigment is 9% of the dry potato starch mass. The aqueous solution containing hydroxy monascus red pigment is mixed with starch paste, and then aged at 2°C for 36h to obtain colloidal retrograded starch containing monascus red pigment; the temperature of the high-pressure treatment is 115°C, and the time of high-pressure treatment is 30min; the obtained colloidal retrograded starch containing monascus red pigment was placed in a water bath with a temperature of 88°C and heated, and 5% of the amount of starch was added with high-temperature amylase (with an a...

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Abstract

The invention discloses a purifying method of hydroxy-containing pigment; the technology thereof is simple, the cost is low, and suitable for industrial production. The method comprises the following steps: pasting and high-pressure processing a starch emulsion to obtain a starch paste, uniformly mixing the obtained starch paste with a hydroxy-containing pigment water solution, aging to obtain a pigment-containing colloidal retrogradation starch; adding a high-temperature amylase into the pigment-containing colloidal retrogradation starch to enzymatically hydrolyzing the starch which is not participated in the retrogradation, separating and discarding the supernatant, collecting the sediment, washing to obtain a purified hydroxy pigment contained retrogradation starch; dissolving the purified hydroxy pigment contained retrogradation starch into a dense alkali liquor to open the hydrogen bond of the starch and make the starch into a water-soluble starch; releasing the pigment containing hydroxy, regulating the pH value thereof to 7.0 in order to neutralize the alkali liquor into a salt; using ethyl alcohol to subside the starch therein, collecting the solution containing the hydroxy pigment; concentrating and drying the obtained solution into a solid; processing the solid by vibration, sieving the separate the block crystal therein, thus the lower layer powder is the purified pigment powder. The method removes the impurities by containing hydroxy; and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a method for purifying hydroxyl-containing pigments. Background technique [0002] Monascus red pigment is a pigment produced during the fermentation process of Monascus sp. At present, the company mainly adopts the method of liquid fermentation of rice flour and spray drying of soybean protein in the later stage. Such products contain a large amount of water-soluble carbohydrates, polypeptides, proteins and other substances that are not utilized by Monascus, which reduces the purity of the pigment, increases the amount of Monascus red pigment added in food, and reduces the stability of the product. [0003] Existing methods for the separation and purification of monascus red pigment include column chromatography, thin plate chromatography, liquid chromatography, etc. It is difficult to separate homologous pigments, orange pigments, yellow pigments, amino acids and protein sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 连喜军董世瑞林旭辉
Owner TIANJIN UNIV OF COMMERCE
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