A plant-derived volatile oil extract with gas-phase free radical scavenging activity and its preparation method
A plant source, volatile oil technology, applied in the direction of essential oils/fragrances, fat production, etc., can solve the problem of weak antioxidant effect, achieve low cost, fresh air, and delay the effect of the body
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Embodiment 1
[0028] Example 1: Extraction of plant volatile oil and its ability to scavenge DPPH free radicals in the gas phase system.
[0029] 1. Extraction of plant volatile oil
[0030] The steam distillation method is adopted, the weight ratio of raw materials and water is 1:4-5, the extraction temperature is 150-170°C, and the extraction time is 5-7h. During the heating process, the steam is introduced into the condenser through the gooseneck on the top of the pot to obtain water and The liquid mixture of volatile oil can be obtained after oil-water separation. The results are as follows
[0031] Table 2 Yield of volatile oil
[0032] Variety of volatile oil Materials (g) Yield (ml) Yield (%) Tea Tree Flower Essential Oil 500 1.0 0.2 Bayberry leaf essential oil 500 0.2 0.04 Lavender 500 1.2 0.24 Bergamot essential oil 500 0.4 0.08 Aiye 500 0.9 0.18 clove 500 1.3 0.26 lemon essential oil 500 2.3 0.46 sweet ...
Embodiment 2
[0050] Example 2: Experiments on free radical scavenging synergies of two-to-two compounding of different plant volatile oils.
[0051] According to the EC50 (half free radical scavenging effect concentration) of different plant volatile oils calculated in Example 1, the yield of volatile oil extracted from different plant raw materials is converted into raw material content, and the concentration ratio of 1:1 is cross compounded in pairs. The extraction temperature is 150-170°C and the extraction time is 5-7h, and the volatile oil is obtained through oil-water separation. After the DPPH free radical scavenging experiment in the gas phase system, the specific operation is as follows: ①Take 2-3 mL of DPPH ethanol solution (concentration: 25 μg / mL) and drop it on the bottom of a 100 mL Erlenmeyer flask. Drop 50 μL of the solution onto the gauze, hang the gauze in the conical flask quickly, plug the cork of the conical flask, seal the cork with plastic wrap, react for a certain p...
Embodiment 3
[0057] Example 3: Extraction of mixed volatile oils from bayberry leaves, pomelo peels and orange peels and their free radical scavenging activity.
[0058] According to the synergistic experiment result of embodiment 2, select three kinds of materials of red bayberry leaf, pomelo peel and orange peel, according to the parts by weight (by wet weight) of bayberry leaf raw material 25-50 parts, the parts by weight (by wet weight) of grapefruit peel by weight) 2-7 parts, orange peel by weight (wet weight) 10-20 parts, crushed and mixed, with a material-to-water ratio of 1:4-5, at an extraction temperature of 150-170°C, for an extraction time of At 5-7h, the volatile oil was obtained by oil-water separation. After the DPPH free radical scavenging experiment in the gas phase system, the specific operation is as follows: ①Take 2-3 mL of DPPH ethanol solution (concentration: 25 μg / mL) and drop it on the bottom of a 100 mL Erlenmeyer flask. Drop 50 μL of the solution onto the gauze, ...
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