Romidepsin production method and fermentation medium thereof

Abstract

The invention discloses a romidepsin production method and a fermentation medium thereof. The fermentation medium for romidepsin production includes a carbon source, an organic nitrogen source and an inorganic phosphate; the carbon source comprises glucose and mannitol, and the organic nitrogen source comprises corn steep liquor, casein and soybean powder; the carbon source accounts for 4-10% of the weight of the fermentation medium; the organic nitrogen source accounts for 2-5% of the weight of the fermentation medium; and the inorganic phosphate accounts for 0.1-2.1% of the weight of the fermentation medium. The invention also discloses the romidepsin production method. When the fermentation medium is used in the romidepsin production, the output is increased to 500-600mg / L and is above 150-200% higher than the output realized by using fermentation media in the prior art.

Description

technical field

[0001] The invention relates to a method for producing romidepsin and a fermentation medium. Background technique

[0002] Romidepsin is a natural polypeptide isolated from the fermentation broth of Chromobacterium violaceum, and its molecular formula is C 24 h 36 N 4 o 6 S 2 , with a molecular weight of 540.

[0003] Romidepsin is a new type of histone deacetylase (HDAC) inhibitor. HDAC is a kind of zinc ion amidase. When catalyzing histone deacetylation, zinc ion is chelated with the hydrate of oxygen on the acetyl group The excessive or abnormal regulation of HDAC leads to excessive deacetylation of histones, and the condensation of chromosomes inhibits transcription, resulting in a decrease in the expression of corresponding genes, leading to tumorigenesis. The anti-tumor mechanism of romidepsin is that after romidepsin enters the cell, its disulfide bond is reduced to a sulfhydryl group by reducing glutathione (GSH), and the sulfhydryl group can co...

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