Dual-wavelength shearing interference digital holography microscope measuring device and method thereof

Abstract

The invention discloses a dual-wavelength shearing interference digital holography microscope measuring device and a method of the measuring device. Laser emergent from two laser devices irradiates on a beam splitter prism from different directions, and exits from the same face of the beam splitter prism, irradiates on the same point on a detected object, then two coincident laser spots are obtained through amplification of a microobjective, then a plane reflecting mirror is used for irradiating two beams of laser on a plane optical flat, the laser is reflected on the front surface and the rear surface of the plane optical flat, the reflected laser vertically irradiates on a CCD camera, and the CCD camera is used for recording a hologram of the single beam of the laser. Matlab software is used for carrying out Fourier transformation on the holograms, positive one-level frequency spectrums of the laser of two wavelengths are extracted, inverse Fourier transformation is carried out on extracted information to obtain the holograms, an angular spectrum method is used for representing the holograms, phase positions are extracted, and further operation is carried out by subtraction of the phase positions to obtain three-dimensional height information of the detected object. The measuring device has the advantages of being simple in laser path, high in system stability, simple in representing procedure, and suitable for the fields of biomedical sciences and material sciences.

Description

technical field [0001] The invention belongs to the technical field of digital holographic microscopy and imaging, and specifically relates to a digital holographic microscopy device and method based on shear interference and dual-wavelength optical phase unwrapping, which are used to measure three-dimensional height information of dispersedly arranged tiny objects. Background technique [0002] Digital holography technology is the product of the combination of computer technology and traditional optical holography. Using digital recording and reproduction methods, the amplitude information and phase information of the object can be obtained more conveniently, and the phase information is an important parameter for restoring the three-dimensional shape of the object. [0003] Traditional microscopic techniques mainly observe tiny objects through optical microscopes. But this can only obtain the distribution of light intensity, and the imaging focal depth of the optical micro...

AI Technical Summary

Problems solved by technology

At present, the optical path of digital holographic microscopic imaging mostly adopts Mach-Zehnder interference optical path, which is difficult due to the complex optical path and cumbersome phase unwrapping algorithm, especially the use of beam splitting prism in the optical path makes the light of object light and reference light The distance is different from the light intensity, which brings more noise into the interference system and reduces the stability of the system

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