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A method for determining the fatty acid composition of microalgae single algal fall

A fatty acid and fatty acid methyl ester technology, which is applied in the field of energy microalgae, can solve the problems of complex operation, long processing time, and unsuitability for rapid detection, and achieve the effects of less sample consumption, reduced operation steps, and shortened methylation time

Active Publication Date: 2016-06-01
中科榆林能源技术运营有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing methods are complicated to operate and take a long time to process, which is not suitable for rapid detection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The same algae powder sample of Isochrysis Zhanjiang was selected, and the conventional acid-catalyzed transesterification method was compared with this method to verify the feasibility of this method.

[0034] Weigh two parts of about 5 mg Chrysophylla algae powder, one part is 5.7 mg, add 5 ml of sulfuric acid-methanol solution with a volume concentration of 1%, heat it in a water bath at 70 ° C, and react for 1 hour. After the reaction system drops to room temperature, add 0.75 ml Deionized water and 2ml of n-hexane were fully oscillated and mixed, and after standing for stratification, the upper hexane layer was taken to analyze the fatty acid methyl ester components by gas chromatography;

[0035] Another part uses this method for transesterification, and adds 1ml of saturated KOH-CH to the weighed 4.9mg of algae powder 3 OH solution, ultrasonic cleaning assisted shock reaction for 5min, then add 1ml of n-hexane, shake and extract, after standing still, the hexane ...

Embodiment 2

[0041] Three types of microalgae, Dunaliella salina, Tetragalenia subcordiformum and Chrysalis dinoflagellate were selected to investigate the general applicability of this method for transesterification of single algae colonies.

[0042] Take the single algal colonies of the above three algae species: 8 μg of Dunaliella salina, 10 μg of Tetraphyllum subcardioid, and 12 μg of Chrysoflagellate, and add them to 1ml of saturated KOH-CH 3 OH solution, ultrasonic cleaning assisted shock reaction for 5min, then add 1ml of n-hexane, shake and extract, after standing still, the hexane layer is fatty acid methyl ester, collect the n-hexane layer, use gas chromatography to analyze its fatty acid methyl ester components (using standard comparison).

[0043] Table 2. Analysis results of fatty acid composition obtained by using this method for three kinds of microalgae

[0044] component name

Embodiment 3

[0046] Isochrysis Zhanjiang was selected to investigate the feasibility of using this method for transesterification of different colonies of the same microalgae.

[0047] Take 11 μg, 13 μg and 9 μg of three different single algal colonies of Isochrysis zhanjiang cultured in batches, and add them to 1ml of saturated KOH-CH 3 OH solution, ultrasonic cleaning assisted shock reaction for 5min, then add 1ml of n-hexane, shake and extract, after standing still, the hexane layer is fatty acid methyl ester, collect the n-hexane layer, use gas chromatography to analyze its fatty acid methyl ester components (using standard comparison).

[0048] Table 3. Analysis results of fatty acid composition obtained by using this method for three different single algal colonies of the same algae species

[0049] component name

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Abstract

The present invention relates to a method for determining the microalgae single-algae colony fatty acid component composition, wherein technical problems of large sample consumption, more operation steps and long time consuming of the methyl esterification method used during the microalgae grease component analysis process in the prior art are solved with the method of the present invention. According to the method of the present invention, dried algae powder is not required to be prepared, algae oil extraction is not required, and the single-algae colony of the microalgae is directly subjected to a methyl esterification reaction under the saturated alkali catalysis condition, wherein the methyl esterification reaction comprises that a microalgae single-algae colony with the size of about 2-5 mm is added to 1 ml of a saturated KOH-CH3OH solution, saponification and methyl esterification are performed on the single-algae colony sample, 1 ml of n-hexane is added after the complete mixing shaking effect, and extraction is performed to obtain the fatty acid methyl ester, wherein the obtained fatty acid methyl ester can be subjected to gas chromatography analysis. The method of the present invention is applicable for fatty acid component analysis of rapid and online monitoring on Chlorophyta, Chrysophyta, Bacillariophyta and other single-cell algae.

Description

technical field [0001] The invention belongs to the field of energy microalgae, and specifically relates to a microalgae single algae fall as a raw material for rapid analysis of its fatty acid composition composition method. Background technique [0002] At present, the rapid development of China's economy has attracted the attention of the world, but the negative impact of environmental pollution and energy shortage on China's sustained and rapid economic development has become increasingly prominent. In terms of energy. Insufficient fossil energy, especially oil reserves in my country, not only restricts rapid economic development, but even endangers national energy security. [0003] As an important raw material for bioenergy, microalgae have the advantages of large biomass, short growth cycle, easy cultivation, and high lipid content. 2 It plays an important role in the recycling of biomass, and it is also a good material for preparing biomass liquid fuel, which has b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 薛松刘亚男曹旭鹏艾江宁
Owner 中科榆林能源技术运营有限责任公司