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Methods, compositions and devices for supplying dietary fatty acid requirements

A technology of free fatty acid and nutritional composition, which can be applied in the directions of edible oil/fat, edible oil/fat phase, drug combination, etc., and can solve problems such as inefficiency

Active Publication Date: 2018-01-02
ALCRESTA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, endogenous enzymes are highly inefficient at converting ALA to DHA and EPA

Method used

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  • Methods, compositions and devices for supplying dietary fatty acid requirements
  • Methods, compositions and devices for supplying dietary fatty acid requirements
  • Methods, compositions and devices for supplying dietary fatty acid requirements

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0206] Example 1: Specific activity of lipase towards DHA and ARA

[0207] To evaluate the enzymatic activity of various lipases on DHA and / or ARA triglycerides, in two mL glass vials (with a magnetic stir bar) containing 0.1M Tris buffer, pH 7.7 and the substrate DHA or ARA triglycerides experiment in. The reaction was initiated by adding the lipase solution. Lipases were obtained from commercial sources as follows: Rhizopus oryzae (Amano DF-15, AmanoEnzymes Inc., Nagoya, Japan), Chromobacterium viscosus (EMD CalBiochem, EMD Biosciences, Billerica, MA) and Pseudomonas fluorescens (Amano AK, Amano Enzymes Inc., Nagoya, Japan). Other lipases are also available from commercial sources such as Candida rugosa (Amano AY 30 or Amano 30, Amano Enzymes Inc., Nagoya, Japan), Aspergillus niger (Amano DS, Amano Enzymes Inc., Nagoya, Japan), Penicillium salmonella casei (Amano 50, Amano Enzymes Inc., Nagoya, Japan), Rhizomucor miehei (L4277, Sigma-Aldrich), Aspergillus oryzae (62285, S...

example 2

[0211] Example 2: Enzymatic activity of Chromobacterium viscosus and Rhizopus oryzae lipases on DHA, ARA and EPA in infant formula

[0212] To assess the enzymatic activity of CV and RO lipases on DHA, ARA and EPA when supplemented to infant formula, by adding 10 g The powder was dissolved in 35 mL of water to prepare milk-based infant formula. Will contain substrate EPA, 0.1M Tris buffer, pH 7.7 and substrate 2.7mg DHA (Nu-check Prep, Inc. lot number T-310-D7-V) and 5.4mg ARA (Nu-check Prep, Inc. lot number T-295) infant formula into one mL glass vials (with magnetic stir bar). The reaction was initiated by the addition of enzyme (ie lipase); four concentrations of each enzyme were tested. The vial was transferred to a water bath at 37°C placed on a magnetic stirrer. 50 μl of each sample was obtained at different time points - 0, 10, 20, 30, 45 and 60 minutes, and added to an HPLC vial containing 950 μl of HPLC running buffer (30% 10 mM ammonium phosphate buffer, pH 3.0 a...

example 3

[0217] Example 3: Scaled-up hydrolysis of DHA triglycerides and ARA triglycerides

[0218] Lipase was evaluated for its ability to hydrolyze TG-DHA and TG-ARA when scaled up to amounts that could be used to supplement infant formula. Infant formula (milk) was prepared by dissolving 162 g of Enfamil powder in 648 mL of tap water (hot water, temperature 37°C). DHA triglycerides (442mg, final concentration of DHA 0.54g, 1.2% total fat) and ARA triglycerides (885mg, final concentration of ARA 1.08g, 2.4% total fat) and are mixed with infant formula before adding water. The reaction was carried out in a water bath with constant stirring. Fat hydrolysis was initiated by addition of CV or RO lipase. Formula samples were drawn at 0, 15 and 30 minutes and analyzed by RT-PCR for hydrolysis of DHA and ARA as described above. The results are shown in Table 3 below.

[0219] Table 3: TG-DHA and TG-ARA hydrolysis in infant formula

[0220]

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Abstract

The present invention provides nutritional formulas comprising long chain polyunsaturated fatty acids (LC-PUFAs), as well as methods and devices for preparing and / or administering nutritional formulas. In some embodiments, the percentage of LC-PUFA in the nutritional formula is in the form of monoglycerides and / or free fatty acids. In some embodiments, the nutritional formula is free of added lipase. Also provided are methods for providing nutrition to a subject, methods for improving fat absorption, methods for improving cognitive performance, methods for preventing chronic lung disease, and methods for reducing a patient's need for a full gastrointestinal The method of the length of time of external nutrition.

Description

technical field [0001] This patent application claims priority under 35 U.S.C. §119 to U.S. Provisional Application No. 61 / 600,207, filed February 17, 2012, and U.S. Provisional Application No. 61 / 719,173, filed October 26, 2012. Background technique [0002] Long chain fatty acids are critical for human health and development. Long-chain fatty acids are consumed in the diet primarily in the form of triglycerides (TG), in which three long-chain fatty acids are bound to a glycerol molecule via ester linkages. Absorption of long-chain triglycerides first requires the enzymatic action of a lipase, such as pancreatic lipase, which digests triglycerides by hydrolysis, breaking them down into monoglycerides and further into free fatty acids. Once available, these monoglycerides and free fatty acids are taken up by endothelial cells in the small intestine where they undergo re-esterification, then transported to the liver and ultimately to tissues in the body for a variety of phys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A23L29/00A23L33/00
CPCA61K9/0029A23D7/011A23D7/013A23D9/013A23K20/158A23L29/06A23L33/40A23L33/115A23L33/12A61K31/202A61K38/465C12Y301/01C12Y301/00A61P1/18A61P11/00A61P25/28A61P3/00A61P3/02A61P3/06A61P43/00A61P7/00A61J15/0092A23V2002/00A23K50/30B65D81/32B65D1/02B65D41/02B65D85/72A61J15/0003A61J15/0026A61K31/20
Inventor A·L·玛戈林R·歌罗陀B·沙诺伊
Owner ALCRESTA
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