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A kind of extraction method of total erinacein in Hericium erinaceus mycelium

A technology of erinacein and extraction method, which is applied in the compound extraction field of reflux extraction of total erinacein, can solve the problems of low extraction efficiency, achieve high extraction efficiency, optimize the extraction process, and improve the extraction rate

Active Publication Date: 2017-04-12
食健客(白山)冻干制品科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The problem to be solved by the present invention is the low extraction efficiency of the traditional solvent extraction method. A composite extraction method for reflux extraction of total erinacein after a combination of cellulose enzymolysis and acid water immersion is adopted, and scutellarin is used as the Quantitative standard products, improve extraction efficiency, and effectively reduce costs

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  • A kind of extraction method of total erinacein in Hericium erinaceus mycelium
  • A kind of extraction method of total erinacein in Hericium erinaceus mycelium

Examples

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Embodiment 1

[0027] Hericium erinaceus mycelium was dried, crushed and sieved to obtain 60 mesh Hericium erinaceus mycelium powder. Take 200g Hericium erinaceus mycelium powder and add 2L water, adjust the pH value to 4.5 with hydrochloric acid, add 2g cellulase, seal and place in a constant temperature water bath at 50°C for 90min enzymolysis, then take it out and boil for 10 minutes to inactivate the enzyme activity. Adjust the pH value to 2 with hydrochloric acid, seal and let stand for 24h. Transfer to a round-bottomed flask, rotate and evaporate the water, then add 2L of 95v% denatured alcohol to the round-bottomed flask, and ultrasonicate for 10 minutes to fully mix the Hericium erinaceus mycelium powder with the solvent. Reflux extraction, extraction time 40min, gained extract A is filtered twice, the obtained filtrate is settled to 2L with 95v% technical alcohol, obtains extract B, gets 2mL and detects the content of total erinacein according to the method for embodiment 1 , the c...

Embodiment 2

[0029]Hericium erinaceus mycelium was dried, crushed and sieved to obtain 60 mesh Hericium erinaceus mycelium powder. Take 200g Hericium erinaceus mycelium powder and add 2L water, adjust the pH value to 4.5 with hydrochloric acid, add 2g cellulase, seal and place in a constant temperature water bath at 50°C for 90min enzymolysis, then take it out and boil for 10 minutes to inactivate the enzyme activity. Adjust the pH value to 2 with hydrochloric acid, seal and let stand for 24 hours. Transfer to a round-bottomed flask, rotate and evaporate the water, then add 2L of 95v% denatured alcohol to the round-bottomed flask, and ultrasonicate for 10 minutes to fully mix the Hericium erinaceus mycelium powder with the solvent. Reflux extraction, extraction time 20min, gained extract A is filtered twice, the obtained filtrate is settled to 2L with 95v% industrial alcohol, obtains extract B, gets 2mL and detects the content of total erinacein according to the method for embodiment 1 , ...

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Abstract

The invention discloses an efficient extraction method of total erinacine in hericium erinaceus mycelium. The efficient extraction method comprises the following steps: drying and crushing hericium erinaceus mycelium; carrying out enzymolysis with cellulose, soaking with acid water; and finally carrying out reflux extraction with ethanol, so as to prepare a total erinacine extract of the hericium erinaceus mycelium. By adopting a reflux extraction method of combining cellulose enzymolysis with acid water leaching, the efficient extraction method has the advantages of low solvent consumption, high extraction efficiency and the like. The extraction process is optimized, so the extraction rate of the total erinacine in the extracted total erinacine extract of the hericium erinaceus mycelium is significantly improved.

Description

technical field [0001] The invention belongs to the field of food industry, and particularly relates to a composite extraction method for reflux extraction of total erinacein after combined treatment of cellulose enzymolysis and soaking in acid water. Background technique [0002] The erinacein in Hericium erinaceus mycelium powder is a class of terpene glycosides. These compounds show a strong stimulating effect on the synthesis of nerve growth factor (NGF), and the activity is far greater than that of adrenaline. Hericin, which can stimulate NGF synthesis, is expected to be a drug for neuron degenerative diseases such as Alzheimer's disease and peripheral nerve regeneration. [0003] Hericin is usually a white crystal, which is a lipophilic component, easily soluble in lipophilic organic solvents, soluble in alcohol, and hardly soluble in water. [0004] Hericin has a cyathane skeleton (as shown in formula I), which is composed of three rings with a certain angle of 5-6-7...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H15/256
Inventor 何晋浙沈强孙培龙陈洪波
Owner 食健客(白山)冻干制品科技有限公司
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