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High-yield antibacterial peptide bacillus licheniformis and application thereof

A technology of bacillus licheniformis and microbial preparations, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of less applied research and few bacillus licheniformis, and achieve the effects of promoting digestion and absorption, enhancing non-specific immunity, and promoting growth

Active Publication Date: 2015-04-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are not many domestic research reports on Bacillus licheniformis, and the research mainly focuses on the fermentation process and production products, and there are few researches on its application in animal growth

Method used

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  • High-yield antibacterial peptide bacillus licheniformis and application thereof
  • High-yield antibacterial peptide bacillus licheniformis and application thereof
  • High-yield antibacterial peptide bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, identification and characteristic study of strains

[0037] 1. Experimental method

[0038] 1.1 Experimental materials 10 samples of intestinal contents of healthy piglets from a pig farm in Zhejiang Province.

[0039] 1.2 Medium

[0040] ①Counting medium (both mass concentration g / L): peptone 10g, beef extract 5g, sodium chloride 5.0g, agar 15.0g, distilled water to 1.0L, pH 7.2, 121℃, 15min sterilization.

[0041] ②LB medium (both mass concentration g / L): Peptone 20g, K 2 HPO 4 .3H 2 O 2.5g, MgSO 4 0.73g, glycerol 15.0ml, agar 15.0g, distilled water to make the volume to 1.0L; pH 7.0, 121℃, 15min sterilization.

[0042] 1.3 Specific experimental methods

[0043] 1) Take 10g of the intestinal contents of healthy piglets from feces samples and add them to 90mL PBS buffer and put them in a water bath at 80°C for 10 minutes to kill non-spore bacteria, then put in 20ml LB liquid medium and culture at 37°C for 24h;

[0044] 2) Dilute the samples to 10 -3 , 10 -5 , 1...

Embodiment 2

[0072] Example 2 Application of Bacillus licheniformis instead of antibiotics in weaned piglets

[0073] 1. Purpose and significance: Weaned piglets were selected as the test subjects to explore the effect of Bacillus licheniformis on the growth performance and diarrhea rate of weaned piglets without antibiotics.

[0074] 2. Materials and methods:

[0075] Test animals: 60 healthy weaned piglets of 23±2 days old Du×Long×Large three-way cross, weighing 7.8±0.2Kg.

[0076] Bacillus licheniformis preparation (animal microbial preparation): Bacillus licheniformis ZJU-X1 is obtained by drying after liquid fermentation, corn flour is the carrier, and the number of viable bacteria of the prepared Bacillus licheniformis preparation is 10 11 cfu / g. In this case, the Bacillus licheniformis preparation is used as an animal additive.

[0077] Remarks: The process steps and parameters of the above-mentioned liquid fermentation of Bacillus licheniformis ZJU-X1 are: inoculate the seed solution in the ...

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PUM

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Abstract

The invention discloses a bacillus licheniformis, which is bacillus licheniformis ZJU-X1, is preserved in China general microbiological culture collection, and the preservation registration mark is CGMCC No. 9688. The invention also discloses a bacillus licheniformis preparation prepared by bacillus licheniformis, the bacillus licheniformis preparation is taken as a feed additive. The invention also provides an animal microbe preparation prepared by bacillus licheniformis, the animal microbe preparation is taken as antibiotic for replacing a weaning piglets material; in the animal microbe preparation, the live bacteria amount of the Bacillus licheniformis preparation is 1011cfu / g.

Description

Technical field [0001] The invention relates to the field of feed additives--microbial preparations, in particular to an efficient and safe Bacillus licheniformis and its application. Background technique [0002] Feed antibiotics have significant effects in promoting animal growth, improving feed conversion efficiency, reducing food loss, and increasing pork production. However, with the widespread use of antibiotics as animal growth promoters in feed, its disadvantages are becoming increasingly prominent, such as causing imbalance of the animal’s gastrointestinal flora, drug resistance and drug residues, etc., triggering a series of public safety and environmental protection. Protection and quality of livestock and poultry products. It has become one of the hot spots in the field of animal nutrition to find non-toxic, efficient, and non-drug resistant green and safe feed additives. [0003] Bacillus licheniformins is one of the feed-grade strains approved by the Ministry of Agr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/17A23K1/18C12R1/10A23K20/195
CPCC12N1/205C12R2001/10
Inventor 汪以真宗鑫王腾浩
Owner ZHEJIANG UNIV