A kind of dendrobium candidum tissue culture medium and preparation method thereof

A technology for Dendrobium officinale and tissue culture seedlings is applied in the directions of tissue culture, botanical equipment and methods, biochemical equipment and methods, etc., and can solve the problems of poor sterilization reliability, low transplant survival rate of Dendrobium officinale, and long culture medium sterilization time. and other problems, to achieve the effect of improving industrial production efficiency, shortening disinfection time, and shortening culture cycle

Active Publication Date: 2016-10-05
翁源县天下泽雨农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a kind of culture medium of Dendrobium officinale tissue culture seedling and preparation method thereof, to solve the problems such as the low transplanting survival rate of Dendrobium candidum in the prior art, the medium disinfection time is long, the sterilization reliability is poor etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A culture medium for tissue culture seedlings of Dendrobium officinale, comprising 150 grams of mashed potatoes, 150 grams of mashed apples, 200 grams of mashed bananas, 250 grams of white sugar, 30 grams of ammonium nitrate, 30 grams of potassium nitrate, 3 grams of potassium dihydrogen phosphate, and 3 grams of boric acid , Alanine 3g, Vitamin B 1 3 grams, 0.5 grams of IBA, 0.5 grams of GA, 0.5 grams of 6-BA, 1200 ml of purified water.

[0025] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:

[0026] S1: After peeling the potatoes, apples, and bananas, weigh them according to the above proportions, then use a grinder at a speed of 500r / min for 3 minutes, grind them evenly, and pour the three kinds of slurry into a 5L container ;

[0027] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, alanine, vitamin B 1 , IBA, GA, 6-BA, and purified water were put into...

Embodiment 2

[0030] A culture medium for tissue culture seedlings of Dendrobium candidum, comprising 200 grams of mashed potatoes, 200 grams of mashed apples, 250 grams of mashed bananas, 300 grams of white sugar, 30 grams of ammonium nitrate, 35 grams of potassium nitrate, 4 grams of potassium dihydrogen phosphate, and 4 grams of boric acid , Serine 4g, Vitamin B 2 5 grams, IBA0.5 grams, GA0.5 grams, 6-BA1 grams, 1350 ml of purified water.

[0031] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:

[0032] S1: After peeling the potatoes, apples, and bananas, weigh them according to the above proportions, then use a grinder at a speed of 550r / min for 2.5 minutes, grind them evenly, and pour the three kinds of slurry into a 5L container middle;

[0033] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, serine, vitamin B 2 , IBA, GA, 6-BA, and purified water are put into the 5L con...

Embodiment 3

[0036] A culture medium for dendrobium candidum tissue culture seedlings, comprising 250 grams of mashed potatoes, 250 grams of apple puree, 300 grams of banana puree, 300 grams of white sugar, 35 grams of ammonium nitrate, 40 grams of potassium nitrate, 5 grams of potassium dihydrogen phosphate, and 4 grams of boric acid , Glycine 5g, Vitamin B 6 5 grams, 1 gram of IBA, 0.5 grams of GA, 1 gram of 6-BA, 1500 ml of purified water.

[0037] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:

[0038]S1: After peeling the potatoes, apples, and bananas, weigh them according to the above ratio, and then use a grinder at a speed of 600r / min for 2 minutes to grind them evenly and pour the three kinds of slurry into a 5L container ;

[0039] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, glycine, vitamin B 6 , IBA, GA, 6-BA, and purified water were put into the 5L container...

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PUM

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Abstract

The invention discloses a culture medium for culturing dendrobium officinale tissue cultured seedling and a method for preparing the culture medium, and belongs to the technical field of dendrobium officinale planting. The culture medium for culturing the dendrobium officinale tissue cultured seedling comprises the following components: mashed potatoes, applesauce, banana sauce, white sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, amino acids, vitamins, IBA, GA, 6-BA and pure water. According to the invention, growth hormones IBA, GA and 6-BA are added to the culture medium for culturing dendrobium officinale tissue cultured seedling, so that the roots, buds and leaves can be promoted to grow quickly; and in addition, the culture medium is rational in component match and comprehensive in nutrition, and can shorten the culturing period of the dendrobium officinale and increase the survival rate of the transplanted dendrobium officinale to 98.75%. The method for preparing the culture medium for culturing the dendrobium officinale tissue cultured seedling, provided by the invention can shorten the disinfection time, improve the sterilization effect reliability and the industrialized production efficiency, and reduce the production cost. The method for preparing the culture medium for culturing the dendrobium officinale tissue cultured seedling is simple to operate and liable to realize the industrialized, normalized and standardized production.

Description

【Technical field】 [0001] The invention belongs to the technical field of dendrobium candidum planting, and in particular relates to a medium for tissue culture seedlings of dendrobium candidum and a preparation method thereof. 【Background technique】 [0002] Dendrobium officinale is a plant in the orchid family. Dendrobium officinale can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium officinale is the best of dendrobium. It is named for its iron-green skin. It grows in the shade of cliffs. The roots do not penetrate the soil, and have the functions of nourishing yin and body, strengthening the body, nourishing the spleen and stomach, protecting the liver and gallbladder, strengthening the tendons and reducing fat, lowering blood sugar, inhibiting tumors, brightening eyes, nourishing the skin, and prolonging life. "Chinese Pharmacy Dictionary" states that Dendrobium candidum "specially treats the gas and liquid of the lung and stomac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 李志山
Owner 翁源县天下泽雨农业科技有限公司
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