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Method of producing biological feed by fermenting banana stem and leaf through micro-organism

A technology of banana stems and leaves and biological feed, applied in animal feed, animal feed, application, etc., can solve the problems of high production cost and low temperature requirements, and achieve the effects of simple operation, deodorization, and great promotion significance

Inactive Publication Date: 2015-04-15
俸祥仁 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No need for anaerobic environment, low temperature requirements, etc., overcome the shortcomings of the existing banana stem and leaf fermentation technology that requires anaerobic conditions and high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Take commercially available Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast bacteria powder, and inoculate them in conventional culture medium to activate the strains, and the activation time is 48 hours to obtain the activated bacteria liquid;

[0015] (2) Take the above-mentioned activated bacteria liquid and carry out 5 successive strain number amplifications at a ratio of 1:10 to 20 in a conventional culture medium, and cultivate it as a working seed strain in an environment of 28 to 36°C;

[0016] (3) Take the above-mentioned working seed strains of Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast respectively to prepare mixed working seed strains in a ratio of 12:9:8:5:8 liquid, so that the number of beneficial bacteria in the mixed working seed strain liquid reaches 15 billion cfu / ml;

[0017] (4) dissolving protease and amylase preparations in the above-mentioned dilute mixed working seed str...

Embodiment 2

[0021] (1) Take commercially available Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast bacteria powder, and inoculate them in conventional culture medium to activate the strains, and the activation time is 24 hours to obtain the activated bacteria liquid;

[0022] (2) Take the above-mentioned activated bacteria liquid and carry out 3 successive strain number amplifications in the ratio of 1:10-20 in the conventional culture medium, and cultivate it as the working seed strain liquid under the environment of 28-36°C;

[0023] (3) Take the above-mentioned working seed strains of Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast respectively in a ratio of 12:12:5:8:12 to prepare a mixed working seed strain liquid, so that the number of beneficial bacteria in the mixed working seed strain liquid reaches 20 billion cfu / ml;

[0024] (4) dissolving cellulase, non-starch polysaccharide enzyme and amylase preparation in t...

Embodiment 3

[0028] (1) Take commercially available Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast bacteria powder, and inoculate them in conventional culture medium to activate the strains, and the activation time is 36 hours to obtain the activated bacteria liquid;

[0029] (2) Take the above-mentioned activated bacteria liquid and carry out 5 successive strain number amplifications at a ratio of 1:10 to 20 in a conventional culture medium, and cultivate it as a working seed strain in an environment of 28 to 36°C;

[0030] (3) Take the working seed strains of the above-mentioned Lactococcus, Candida utilis, Bacillus subtilis, Bacillus licheniformis, and yeast respectively in a ratio of 5:5:4:4:3 to prepare a mixed working seed strain liquid, so that the number of beneficial bacteria in the mixed working seed strain liquid reaches 15 billion cfu / ml;

[0031] (4) dissolving cellulase and non-starch polysaccharase respectively in the above-mentioned dilute...

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Abstract

The invention discloses a method of producing a biological feed by fermenting banana stems and leaves through micro-organisms. The method mainly includes following steps: (1) respectively inoculating marketed bacterial powders of lactococcus lactis, candida utilis, bacillus subtilis, bacillus licheniformis and yeasts onto a common culture medium for performing bacterial strain activation with an activation time being 24-48 h to obtain an activated bacterial liquid; (2) performing gradually culture number amplification to the activated bacterial liquid for 2-5 times according to a proportion of 1:10-20 to culture a work seed bacterial strain liquid; (3) preparing a mixed work seed bacterial strain liquid; (4) respectively dissolving an enzyme preparation in the diluted mixed work seed bacterial strain liquid to obtain a biological fermentation liquid; (5) spraying the biological fermentation liquid onto the surface of roll-grinded and cut banana stems and leaves according to a usage amount of 500-1000g on per ton of the banana stems and leaves with stirring; and (6) performing fermentation under a humidity of 50-80% at 10-36 DEG C for 15-30 days to obtain the biological feed.

Description

Technical field: [0001] The invention relates to a method for producing biological feed by using microorganisms to ferment banana stems and leaves, and belongs to the technical field of microorganism fermentation. technical background: [0002] Guangxi is a large agricultural province with abundant crop straw resources, with an annual output of more than 60 million tons of crop straw, mainly including rice straw, sugarcane tops, corn straw, cassava straw, peanut straw, and cassava residue. Guangxi is also a major province of banana planting and sugarcane production in the country, and a large amount of straw and processing residues are also produced during the planting and processing of related crops. Taking 2009 as an example, about 134,700 hectares of mulberry trees were planted in Guangxi. According to the general calculation of felling twice a year, the average dry mulberry branches felled per hectare was about 18 tons. The planting area of ​​raw sugarcane is about 800,...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00A23K1/16
Inventor 俸祥仁
Owner 俸祥仁
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