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Caffeine extract of aegilops variabilis, and extraction method and applications thereof

A technology of variable goat grass and extraction method, applied in the field of caffeine extract and its extraction, to achieve the effect of high product purity, low critical pressure and temperature, and less dosage

Inactive Publication Date: 2015-04-22
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, domestic research has not reported caffeine metabolism related to the resistance of gramineous plants to pathogenic nematodes. With the development of transcriptome sequencing technology in recent years, there have been reports on the use of transcriptome technology combined with comparative genome technology to study plant resistance. growing year by year

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  • Caffeine extract of aegilops variabilis, and extraction method and applications thereof
  • Caffeine extract of aegilops variabilis, and extraction method and applications thereof
  • Caffeine extract of aegilops variabilis, and extraction method and applications thereof

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Embodiment 1

[0028] 1. Experimental materials and reagents

[0029]1. Sequenced plant material: Select Goatweed variabilis (MQ Yu et al (1990) Agronomie, 6, 451-456) with CCN resistance and RKN resistance as the transcriptome sequencing material to determine its effect on inoculation of CCN treatment and non-inoculation The expression of all genes in roots under two conditions of CCN. First, the seeds of the material are subjected to surface sterilization treatment (soaked in the mixed solution of 3% sodium hypochlorite and 0.01% Tween20 for 5 minutes and then soaked 3 times with sterilized water, and then the seeds are evenly spread on the continuously moist filter paper and placed In a petri dish with a diameter of 5 cm, seedlings were germinated at a room temperature of about 20°C and a photoperiod of 16h / 8h. Ten days later, the seedlings were divided into two groups: a group of J2 larvae (H.avenae) for inoculation with CCN ( Each plant was inoculated with 1000), another group was not ...

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Abstract

The invention discloses a caffeine extract of aegilops variabilis, and an extraction method and applications thereof, and belongs to the field of bioengineering. The extraction method comprises following specific steps: aegilops variabilis root is collected, washed, cut into slices, dried in a drying oven at 80 DEG C for 6h so as to remove water completely, smashed using a pulverizer, and sieved using a 60-mesh sieve; 10g of an obtained aegilops variabilis root powder is delivered into a 50ml extraction kettle, absolute ethyl alcohol is taken as an entrainer, and extraction is performed in a CO2 extractor at 30MPa, 60DEG C for 2h so as to obtain the caffeine extract of aegilops variabilis, wherein flow rate of CO2 is controlled to be 1.5LPM. It is shown that: caffeine with a concentration more than 0.1% possesses inhibition effects on plant pathogenic nematodes, and the caffeine extract of aegilops variabilis can be used for preparing insect repellents used for preventing heterodera avenae sensu lato and meloidogyne incognita chitwood.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a caffeine extract of goat grass vulgaris and its extraction method and application. Background technique [0002] Caffeine (Caffeine, caffeine) is a xanthine alkaloid, and there are two common types of caffeine water and caffeine anhydrous. Caffeine is a stimulant for the central nervous system. Caffeine is mainly found in certain kinds of plants and is an alkaloid. By paralyzing insects that invade plants, it acts as an insecticide, protecting plants from pests and diseases. At first, people in the world mainly obtained caffeine from coffee beans, and its content varied according to different types of coffee beans and different production processes. It was later discovered that tea also contains caffeine, and the amount of caffeine contained in a tea usually depends on the strength of the tea made. Therefore, the caffeine content in green tea, black tea, and Pu'er tea is diffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/44A01P5/00
Inventor 余懋群郑明辉李林龙海徐德林潘志芬邓光兵
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S